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styrofoam container for liquid nitrogen  (Fisher Scientific)
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<t>Liquid</t> <t>Nitrogen</t> Tissue Processing for Human Breast Tissue Pulverization (A) Equipment setup for tissue processing showing mortar and pestle. The mortar and pestle are pre-cooled with liquid nitrogen before use to ensure optimal freezing of the tissue. (B) Tissue preparation materials including sterile petri dishes and forceps for tissue handling and dissection. These sterile tools are essential for maintaining aseptic conditions. (C) Liquid nitrogen <t>container.</t> This specialized insulated container is used for safe storage and dispensing of liquid nitrogen, which is crucial for flash-freezing tissue and maintaining extremely low temperatures during the grinding process. (D) Orange bowl containing ice for pre-chilling microcentrifuge tubes and maintaining the frozen state of the tissue sample. Maintaining cold temperatures throughout the process is critical for sample integrity. (E) Mortar and pestle with liquid nitrogen. This image shows the pre-cooled mortar, now filled with liquid nitrogen, ready to receive the tissue sample. The liquid nitrogen rapidly freezes the tissue, making it brittle and amenable to pulverization. (F) Tissue sample in mortar with liquid nitrogen being added. The tissue must become completely frozen and brittle (typically 1-2 minutes) before grinding begins to ensure efficient and uniform pulverization into a fine powder. (G) Grinded tissue with pestle in mortar. Pulverized tissue powder in the mortar after grinding. The tissue should be reduced to a fine, homogeneous powder before collection. (H) Collection of pulverized tissue in pre-chilled microcentrifuge tube. After collection, the tube is immediately closed and placed on dry ice to prevent thawing and degradation of the sample. (I) Resuspension of tissue powder in PG Shield buffer. The suspension is gently mixed and incubated at room temperature for 30 minutes with periodic inversion to ensure complete penetration and stabilization of the tissue fragments by the buffer.
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liquid nitrogen cooled ccd camera  (Princeton Instruments)
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<t>Liquid</t> <t>Nitrogen</t> Tissue Processing for Human Breast Tissue Pulverization (A) Equipment setup for tissue processing showing mortar and pestle. The mortar and pestle are pre-cooled with liquid nitrogen before use to ensure optimal freezing of the tissue. (B) Tissue preparation materials including sterile petri dishes and forceps for tissue handling and dissection. These sterile tools are essential for maintaining aseptic conditions. (C) Liquid nitrogen <t>container.</t> This specialized insulated container is used for safe storage and dispensing of liquid nitrogen, which is crucial for flash-freezing tissue and maintaining extremely low temperatures during the grinding process. (D) Orange bowl containing ice for pre-chilling microcentrifuge tubes and maintaining the frozen state of the tissue sample. Maintaining cold temperatures throughout the process is critical for sample integrity. (E) Mortar and pestle with liquid nitrogen. This image shows the pre-cooled mortar, now filled with liquid nitrogen, ready to receive the tissue sample. The liquid nitrogen rapidly freezes the tissue, making it brittle and amenable to pulverization. (F) Tissue sample in mortar with liquid nitrogen being added. The tissue must become completely frozen and brittle (typically 1-2 minutes) before grinding begins to ensure efficient and uniform pulverization into a fine powder. (G) Grinded tissue with pestle in mortar. Pulverized tissue powder in the mortar after grinding. The tissue should be reduced to a fine, homogeneous powder before collection. (H) Collection of pulverized tissue in pre-chilled microcentrifuge tube. After collection, the tube is immediately closed and placed on dry ice to prevent thawing and degradation of the sample. (I) Resuspension of tissue powder in PG Shield buffer. The suspension is gently mixed and incubated at room temperature for 30 minutes with periodic inversion to ensure complete penetration and stabilization of the tissue fragments by the buffer.
Liquid Nitrogen Cooled Ccd Camera, supplied by Princeton Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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liquid nitrogen cooled ccd camera - by Bioz Stars, 2026-07
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liquid nitrogen cooled camera  (Princeton Instruments)
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<t>Liquid</t> <t>Nitrogen</t> Tissue Processing for Human Breast Tissue Pulverization (A) Equipment setup for tissue processing showing mortar and pestle. The mortar and pestle are pre-cooled with liquid nitrogen before use to ensure optimal freezing of the tissue. (B) Tissue preparation materials including sterile petri dishes and forceps for tissue handling and dissection. These sterile tools are essential for maintaining aseptic conditions. (C) Liquid nitrogen <t>container.</t> This specialized insulated container is used for safe storage and dispensing of liquid nitrogen, which is crucial for flash-freezing tissue and maintaining extremely low temperatures during the grinding process. (D) Orange bowl containing ice for pre-chilling microcentrifuge tubes and maintaining the frozen state of the tissue sample. Maintaining cold temperatures throughout the process is critical for sample integrity. (E) Mortar and pestle with liquid nitrogen. This image shows the pre-cooled mortar, now filled with liquid nitrogen, ready to receive the tissue sample. The liquid nitrogen rapidly freezes the tissue, making it brittle and amenable to pulverization. (F) Tissue sample in mortar with liquid nitrogen being added. The tissue must become completely frozen and brittle (typically 1-2 minutes) before grinding begins to ensure efficient and uniform pulverization into a fine powder. (G) Grinded tissue with pestle in mortar. Pulverized tissue powder in the mortar after grinding. The tissue should be reduced to a fine, homogeneous powder before collection. (H) Collection of pulverized tissue in pre-chilled microcentrifuge tube. After collection, the tube is immediately closed and placed on dry ice to prevent thawing and degradation of the sample. (I) Resuspension of tissue powder in PG Shield buffer. The suspension is gently mixed and incubated at room temperature for 30 minutes with periodic inversion to ensure complete penetration and stabilization of the tissue fragments by the buffer.
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liquid nitrogen  (Air Liquide)
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<t>Liquid</t> <t>Nitrogen</t> Tissue Processing for Human Breast Tissue Pulverization (A) Equipment setup for tissue processing showing mortar and pestle. The mortar and pestle are pre-cooled with liquid nitrogen before use to ensure optimal freezing of the tissue. (B) Tissue preparation materials including sterile petri dishes and forceps for tissue handling and dissection. These sterile tools are essential for maintaining aseptic conditions. (C) Liquid nitrogen <t>container.</t> This specialized insulated container is used for safe storage and dispensing of liquid nitrogen, which is crucial for flash-freezing tissue and maintaining extremely low temperatures during the grinding process. (D) Orange bowl containing ice for pre-chilling microcentrifuge tubes and maintaining the frozen state of the tissue sample. Maintaining cold temperatures throughout the process is critical for sample integrity. (E) Mortar and pestle with liquid nitrogen. This image shows the pre-cooled mortar, now filled with liquid nitrogen, ready to receive the tissue sample. The liquid nitrogen rapidly freezes the tissue, making it brittle and amenable to pulverization. (F) Tissue sample in mortar with liquid nitrogen being added. The tissue must become completely frozen and brittle (typically 1-2 minutes) before grinding begins to ensure efficient and uniform pulverization into a fine powder. (G) Grinded tissue with pestle in mortar. Pulverized tissue powder in the mortar after grinding. The tissue should be reduced to a fine, homogeneous powder before collection. (H) Collection of pulverized tissue in pre-chilled microcentrifuge tube. After collection, the tube is immediately closed and placed on dry ice to prevent thawing and degradation of the sample. (I) Resuspension of tissue powder in PG Shield buffer. The suspension is gently mixed and incubated at room temperature for 30 minutes with periodic inversion to ensure complete penetration and stabilization of the tissue fragments by the buffer.
Liquid Nitrogen, supplied by Air Liquide, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/liquid+nitrogen/pmc12933514-39-0-14?v=Air+Liquide
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liquid nitrogen - by Bioz Stars, 2026-07
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Liquid Nitrogen Tissue Processing for Human Breast Tissue Pulverization (A) Equipment setup for tissue processing showing mortar and pestle. The mortar and pestle are pre-cooled with liquid nitrogen before use to ensure optimal freezing of the tissue. (B) Tissue preparation materials including sterile petri dishes and forceps for tissue handling and dissection. These sterile tools are essential for maintaining aseptic conditions. (C) Liquid nitrogen container. This specialized insulated container is used for safe storage and dispensing of liquid nitrogen, which is crucial for flash-freezing tissue and maintaining extremely low temperatures during the grinding process. (D) Orange bowl containing ice for pre-chilling microcentrifuge tubes and maintaining the frozen state of the tissue sample. Maintaining cold temperatures throughout the process is critical for sample integrity. (E) Mortar and pestle with liquid nitrogen. This image shows the pre-cooled mortar, now filled with liquid nitrogen, ready to receive the tissue sample. The liquid nitrogen rapidly freezes the tissue, making it brittle and amenable to pulverization. (F) Tissue sample in mortar with liquid nitrogen being added. The tissue must become completely frozen and brittle (typically 1-2 minutes) before grinding begins to ensure efficient and uniform pulverization into a fine powder. (G) Grinded tissue with pestle in mortar. Pulverized tissue powder in the mortar after grinding. The tissue should be reduced to a fine, homogeneous powder before collection. (H) Collection of pulverized tissue in pre-chilled microcentrifuge tube. After collection, the tube is immediately closed and placed on dry ice to prevent thawing and degradation of the sample. (I) Resuspension of tissue powder in PG Shield buffer. The suspension is gently mixed and incubated at room temperature for 30 minutes with periodic inversion to ensure complete penetration and stabilization of the tissue fragments by the buffer.

Journal: STAR Protocols

Article Title: High-resolution Hi-C profiling of human breast tissues using an optimized protocol for clinical samples

doi: 10.1016/j.xpro.2025.104327

Figure Lengend Snippet: Liquid Nitrogen Tissue Processing for Human Breast Tissue Pulverization (A) Equipment setup for tissue processing showing mortar and pestle. The mortar and pestle are pre-cooled with liquid nitrogen before use to ensure optimal freezing of the tissue. (B) Tissue preparation materials including sterile petri dishes and forceps for tissue handling and dissection. These sterile tools are essential for maintaining aseptic conditions. (C) Liquid nitrogen container. This specialized insulated container is used for safe storage and dispensing of liquid nitrogen, which is crucial for flash-freezing tissue and maintaining extremely low temperatures during the grinding process. (D) Orange bowl containing ice for pre-chilling microcentrifuge tubes and maintaining the frozen state of the tissue sample. Maintaining cold temperatures throughout the process is critical for sample integrity. (E) Mortar and pestle with liquid nitrogen. This image shows the pre-cooled mortar, now filled with liquid nitrogen, ready to receive the tissue sample. The liquid nitrogen rapidly freezes the tissue, making it brittle and amenable to pulverization. (F) Tissue sample in mortar with liquid nitrogen being added. The tissue must become completely frozen and brittle (typically 1-2 minutes) before grinding begins to ensure efficient and uniform pulverization into a fine powder. (G) Grinded tissue with pestle in mortar. Pulverized tissue powder in the mortar after grinding. The tissue should be reduced to a fine, homogeneous powder before collection. (H) Collection of pulverized tissue in pre-chilled microcentrifuge tube. After collection, the tube is immediately closed and placed on dry ice to prevent thawing and degradation of the sample. (I) Resuspension of tissue powder in PG Shield buffer. The suspension is gently mixed and incubated at room temperature for 30 minutes with periodic inversion to ensure complete penetration and stabilization of the tissue fragments by the buffer.

Article Snippet: Styrofoam container for liquid nitrogen , Fisher Scientific , N/A.

Techniques: Sterility, Dissection, Suspension, Incubation