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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) <t>and</t> <t>GFP‐Lifeact</t> (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.
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Image Search Results


GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) and GFP‐Lifeact (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.

Journal: Febs Letters

Article Title: CCT4 promotes tunneling nanotube formation

doi: 10.1002/1873-3468.70190

Figure Lengend Snippet: GFP‐CCT4 expression induces the formation of nanotubes containing F‐actin and tubulin puncta. (A) Live‐imaging of Neuro2A transfected with mCherry‐CCT4 (magenta) and GFP‐Lifeact (green). Arrows indicate a nanotube connecting two cells. Scale bar, 10 μm. (B) Neuro2A was transfected with GFP‐CCT4 (green) and stained with phalloidin (magenta). Arrows indicate the nanotube connecting two cells. Scale bar, 5 μm. (C) Live imaging of Neuro2A cells transfected with mCherry‐CCT4 (magenta) was stained with Mitotracker (green). Arrows indicate mitochondria. Scale bar, 20 μm.

Article Snippet: GFP‐Lifeact was purchased from Addgene (catalog # 58470). mCherry‐EB1 was constructed by subcloning mouse EB1 from mouse brain cDNAs into pmCherry‐N1A by NEB builder.

Techniques: Expressing, Imaging, Transfection, Staining