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Pancreatic islet analysis. A Representative hematoxylin–eosin (HE) staining for morphological evaluation of pancreatic islets at 14–15 and 18–20 weeks of age. B Pancreatic islet area analysis across experimental groups. (* p < 0.05). C Spearman correlation between islets’ area (µm 2 ) and body weight (g). D Representative images for insulin immunohistochemical analysis. E Quantification of insulin-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. <t>BTBR</t> ob/ob ; # vs. SGLT2i; & vs. SGLT2i + CR; $ vs. SGLT2i + CR + BM-MSCs; continuous line indicated difference between time points). Scale bars represent 100 μm in A and D . Error bars represent mean ± SEM; n = 6 animals
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b6.cg-lepob/j mice (ob/ob mice)  (Oriental Yeast Co)
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Pancreatic islet analysis. A Representative hematoxylin–eosin (HE) staining for morphological evaluation of pancreatic islets at 14–15 and 18–20 weeks of age. B Pancreatic islet area analysis across experimental groups. (* p < 0.05). C Spearman correlation between islets’ area (µm 2 ) and body weight (g). D Representative images for insulin immunohistochemical analysis. E Quantification of insulin-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. <t>BTBR</t> ob/ob ; # vs. SGLT2i; & vs. SGLT2i + CR; $ vs. SGLT2i + CR + BM-MSCs; continuous line indicated difference between time points). Scale bars represent 100 μm in A and D . Error bars represent mean ± SEM; n = 6 animals
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Image Search Results


Pancreatic islet analysis. A Representative hematoxylin–eosin (HE) staining for morphological evaluation of pancreatic islets at 14–15 and 18–20 weeks of age. B Pancreatic islet area analysis across experimental groups. (* p < 0.05). C Spearman correlation between islets’ area (µm 2 ) and body weight (g). D Representative images for insulin immunohistochemical analysis. E Quantification of insulin-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. BTBR ob/ob ; # vs. SGLT2i; & vs. SGLT2i + CR; $ vs. SGLT2i + CR + BM-MSCs; continuous line indicated difference between time points). Scale bars represent 100 μm in A and D . Error bars represent mean ± SEM; n = 6 animals

Journal: Stem Cell Research & Therapy

Article Title: Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach

doi: 10.1186/s13287-025-04358-7

Figure Lengend Snippet: Pancreatic islet analysis. A Representative hematoxylin–eosin (HE) staining for morphological evaluation of pancreatic islets at 14–15 and 18–20 weeks of age. B Pancreatic islet area analysis across experimental groups. (* p < 0.05). C Spearman correlation between islets’ area (µm 2 ) and body weight (g). D Representative images for insulin immunohistochemical analysis. E Quantification of insulin-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. BTBR ob/ob ; # vs. SGLT2i; & vs. SGLT2i + CR; $ vs. SGLT2i + CR + BM-MSCs; continuous line indicated difference between time points). Scale bars represent 100 μm in A and D . Error bars represent mean ± SEM; n = 6 animals

Article Snippet: BTBR ob/ob mice (BTBR.Cg-Lepob/WiscJ; 004824-JAX Laboratories, Bar Harbor, ME, USA), homozygous for leptin gene knockout, serve as a model for T2DM and DKD.

Techniques: Staining, Immunohistochemical staining

Immunohistochemical analysis of pancreatic islets. A Representative images of pancreatic sections from experimental groups stained for cleaved caspase-3 at 14–15 and 18–20 weeks of age. B Quantification of cleaved caspase-3-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. BTBR ob/ob ; continuous line indicates difference between time points). Scale bars represent 100 μm in A . Error bars represent mean ± SEM; n = 5 animals

Journal: Stem Cell Research & Therapy

Article Title: Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach

doi: 10.1186/s13287-025-04358-7

Figure Lengend Snippet: Immunohistochemical analysis of pancreatic islets. A Representative images of pancreatic sections from experimental groups stained for cleaved caspase-3 at 14–15 and 18–20 weeks of age. B Quantification of cleaved caspase-3-positive cells per pancreatic islets across groups. ( p < 0.05; * vs. BTBR ob/ob ; continuous line indicates difference between time points). Scale bars represent 100 μm in A . Error bars represent mean ± SEM; n = 5 animals

Article Snippet: BTBR ob/ob mice (BTBR.Cg-Lepob/WiscJ; 004824-JAX Laboratories, Bar Harbor, ME, USA), homozygous for leptin gene knockout, serve as a model for T2DM and DKD.

Techniques: Immunohistochemical staining, Staining

Gene expression of podocyte markers and components of the RAAS and Ang II/Nox4/TRPC6 axis compared to BTBR wild type. A WT-1. B Nphs1. C Synpo2. D Actn4. E Nox4. F Trpc6. G Ace. H Ace2. I Ace2/Ace ratio. (* p < 0.05). Error bars represent mean ± SEM; n = 5–6 animals

Journal: Stem Cell Research & Therapy

Article Title: Combining sodium-glucose co-transporter-2 inhibitor with mesenchymal stem cells and brown adipose tissue (BAT) and white adipose tissue (WAT) transplantation to mitigate the progression of diabetic kidney disease: a pre-clinical approach

doi: 10.1186/s13287-025-04358-7

Figure Lengend Snippet: Gene expression of podocyte markers and components of the RAAS and Ang II/Nox4/TRPC6 axis compared to BTBR wild type. A WT-1. B Nphs1. C Synpo2. D Actn4. E Nox4. F Trpc6. G Ace. H Ace2. I Ace2/Ace ratio. (* p < 0.05). Error bars represent mean ± SEM; n = 5–6 animals

Article Snippet: BTBR ob/ob mice (BTBR.Cg-Lepob/WiscJ; 004824-JAX Laboratories, Bar Harbor, ME, USA), homozygous for leptin gene knockout, serve as a model for T2DM and DKD.

Techniques: Gene Expression