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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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heart rate module ecg lead cable xprezzon  (Spacelabs Healthcare Inc)
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. <t>ECG</t> was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).
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( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. ECG was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).

Journal: Frontiers in Cardiovascular Medicine

Article Title: Heart rate reduction after genetic ablation of L-type Ca v 1.3 channels induces cardioprotection against ischemia-reperfusion injury

doi: 10.3389/fcvm.2023.1134503

Figure Lengend Snippet: ( A ) In vivo experimental protocol applied to wild-type (WT) mice, consisting of 40 min of ischemia induced by ligation of the left coronary artery followed by 60 min of reperfusion. Mice were assigned to receive either 0.9% NaCl (Ctrl, n = 6) or Ivabradine (Iva, 6 mg/kg, n = 7), intraperitoneally 30 min before ischemia. ECG was recorded during the surgical protocol; ( B ) scatter dot plots and mean ± SD were plotted for baseline HR measured before ischemia in control mice (NaCl 0.9%) ( n = 6) or Iva pre-treated mice ( n = 7); ( C ) scatter dot plots and mean ± SD were plotted for infarct size (expressed as the percentage of the area at risk) and measured at the end of the surgical protocol in control (Ctrl, n = 6) and Iva pre-treated (Iva, n = 7) mice subjected to ischemia-reperfusion (IR) protocol; Statistical analysis was performed using the non-parametric Mann–Whitney test. Statistical significance is noted ** for p = 0.0047 for ( B,C ).

Article Snippet: One-lead surface electrocardiograms (ECG) were recorded by Ag/AgCl gel-coated electrodes (Unomedical) attached to the superior right and both inferior limbs of mice and connected to a standard one-lead ECG amplifier module (EMKA Technologies, Paris, France).

Techniques: In Vivo, Ligation, Control, MANN-WHITNEY

( A ) In vivo experimental protocol applied to wild-type (WT), Ca v 1.3 +/− and Ca v 1.3 −/− mice, consisting in 40 min ischemia, induced by ligation of the left coronary artery, followed by 60 min of reperfusion. ECG was recorded during the surgical protocol; ( B ) scatter dot blots and mean ± SD were plotted for baseline HR measured before ischemia for WT mice (NaCl 0.9%) ( n = 6), Ca v 1.3 +/− ( n = 6) and Ca v 1.3 −/− ( n = 7); ( C ) scatter dot plots of infarct size and means ± SD were plotted for all mice subjected to IR protocol. Non parametric Kruskal–Wallis test followed by Dunn's post hoc test was used to compare data among groups. ns is noted for p > 0.05, * for p < 0.05 and *** for p < 0.001 vs. control; ns in second place was noted for p > 0.05 vs. Ca v 1.3 +/− .

Journal: Frontiers in Cardiovascular Medicine

Article Title: Heart rate reduction after genetic ablation of L-type Ca v 1.3 channels induces cardioprotection against ischemia-reperfusion injury

doi: 10.3389/fcvm.2023.1134503

Figure Lengend Snippet: ( A ) In vivo experimental protocol applied to wild-type (WT), Ca v 1.3 +/− and Ca v 1.3 −/− mice, consisting in 40 min ischemia, induced by ligation of the left coronary artery, followed by 60 min of reperfusion. ECG was recorded during the surgical protocol; ( B ) scatter dot blots and mean ± SD were plotted for baseline HR measured before ischemia for WT mice (NaCl 0.9%) ( n = 6), Ca v 1.3 +/− ( n = 6) and Ca v 1.3 −/− ( n = 7); ( C ) scatter dot plots of infarct size and means ± SD were plotted for all mice subjected to IR protocol. Non parametric Kruskal–Wallis test followed by Dunn's post hoc test was used to compare data among groups. ns is noted for p > 0.05, * for p < 0.05 and *** for p < 0.001 vs. control; ns in second place was noted for p > 0.05 vs. Ca v 1.3 +/− .

Article Snippet: One-lead surface electrocardiograms (ECG) were recorded by Ag/AgCl gel-coated electrodes (Unomedical) attached to the superior right and both inferior limbs of mice and connected to a standard one-lead ECG amplifier module (EMKA Technologies, Paris, France).

Techniques: In Vivo, Ligation, Control