Journal: Renal Failure

Article Title: LCZ696, an angiotensin receptor-neprilysin inhibitor, ameliorates epithelial-mesenchymal transition of peritoneal mesothelial cells and M2 macrophage polarization

doi: 10.1080/0886022X.2024.2392849

Figure Lengend Snippet: LCZ696 Effectively improved PF and inhibited the process of EMT in PDF-induced mouse model. (A) Photomicrographs illustrating Masson’s trichrome staining of the peritoneum. (B) Scatter plots showing the Masson’s trichrome staining positive area (%). (C) Scatter plots showing the thickness of peritoneum. (D) Western blot was conducted to evaluate the protein level of α-SMA, Collagen III, Fibronectin, E-cadherin and GAPDH in peritoneum. (E–H) Scatter plots showing the densitometry analysis of α-SMA, Collagen III, Fibronectin and E-cadherin normalized by GAPDH. Data are expressed as mean ± SEM. ** P < 0.01; *** P < 0.001; **** P < 0.0001. N.S., statistically not significant, with the comparisons labeled. Scale bars in (A) = 50 μm.

Article Snippet: LCZ696 (S7678) was purchased from Selleckchem (Houston, TX, USA).

Techniques: Staining, Western Blot, Labeling

Journal: Renal Failure

Article Title: LCZ696, an angiotensin receptor-neprilysin inhibitor, ameliorates epithelial-mesenchymal transition of peritoneal mesothelial cells and M2 macrophage polarization

doi: 10.1080/0886022X.2024.2392849

Figure Lengend Snippet: LCZ696 Inhibits TGF-β1-induced EMT of HPMCs. (A) Western blot was conducted to evaluate the protein level of α-SMA, Collagen I, Collagen III, Fibronectin, E-cadherin and GAPDH in HPMCs cell lysates. (B–F) Scatter plots showing the densitometry analysis of α-SMA, Collagen I, Collagen III, Fibronectin and E-cadherin normalized by GAPDH. (G) Photomicrographs illustrating immunofluorescence staining of α-SMA. (H) The count of α-SMA-positive cells. (I) Western blot was conducted to evaluate the protein level of Snail, Slug and GAPDH in HPMCs cell lysates. (J, K) Scatter plots showing the densitometry analysis of Snail and Slug normalized by GAPDH. Data are expressed as mean ± SEM. ** P < 0.01; *** P < 0.001; **** P < 0.0001. N.S., statistically not significant, with the comparisons labeled. Scale bars in (G) = 50 μm.

Article Snippet: LCZ696 (S7678) was purchased from Selleckchem (Houston, TX, USA).

Techniques: Western Blot, Immunofluorescence, Staining, Labeling

Journal: Renal Failure

Article Title: LCZ696, an angiotensin receptor-neprilysin inhibitor, ameliorates epithelial-mesenchymal transition of peritoneal mesothelial cells and M2 macrophage polarization

doi: 10.1080/0886022X.2024.2392849

Figure Lengend Snippet: LCZ696 Inhibits TGF-β1-induced EMT through the inactivation of the TGFβ/Smad3 signaling pathway. (A) Western blot was conducted to evaluate the protein level of TGFβRI, p-Smad3, Smad3, CTGF and GAPDH in HPMCs cell lysates. (B, D, E) Scatter plots showing the densitometry analysis of TGFβRI, Smad3 and CTGF normalized by GAPDH. (C) Scatter plot showing the densitometry analysis of p-Smad3 normalized by Smad3. Data are expressed as mean ± SEM. **** P < 0.0001. N.S., statistically not significant, with the comparisons labeled.

Article Snippet: LCZ696 (S7678) was purchased from Selleckchem (Houston, TX, USA).

Techniques: Western Blot, Labeling

Journal: Renal Failure

Article Title: LCZ696, an angiotensin receptor-neprilysin inhibitor, ameliorates epithelial-mesenchymal transition of peritoneal mesothelial cells and M2 macrophage polarization

doi: 10.1080/0886022X.2024.2392849

Figure Lengend Snippet: LCZ696 Inhibits TGF-β1-induced EMT through the inactivation of PDGFRβ and EGFR pathway. (A) Western blot was conducted to evaluate the protein level of p-PDGFRβ, PDGFRβ and GAPDH in HPMCs cell lysates. (B) Scatter plot showing the densitometry analysis of p-PDGFRβ normalized by PDGFRβ. (C) Scatter plot showing the densitometry analysis of PDGFRβ normalized by GAPDH. (D) Western blot was conducted to evaluate the protein level of p-EGFR, EGFR and GAPDH in HPMCs cell lysates. (E) Scatter plot showing the densitometry analysis of p-EGFR normalized by EGFR. (F) Scatter plot showing the densitometry analysis of EGFR normalized by GAPDH. Data are expressed as mean ± SEM. ** P < 0.01; *** P < 0.001; **** P < 0.0001. N.S., statistically not significant, with the comparisons labeled.

Article Snippet: LCZ696 (S7678) was purchased from Selleckchem (Houston, TX, USA).

Techniques: Western Blot, Labeling

Journal: Renal Failure

Article Title: LCZ696, an angiotensin receptor-neprilysin inhibitor, ameliorates epithelial-mesenchymal transition of peritoneal mesothelial cells and M2 macrophage polarization

doi: 10.1080/0886022X.2024.2392849

Figure Lengend Snippet: LCZ696 Blocks M2 macrophage polarization by regulating STAT6 signaling pathway. (A) Western blot was conducted to evaluate the protein level of Arginase-1, CD163, p-STAT6, STAT6 and GAPDH in Raw264.7 cell lysates. (B, C, E) Scatter plots showing the densitometry analysis of Arginase-1, CD163 and STAT6 normalized by GAPDH. (D) Scatter plot showing the densitometry analysis of p-STAT6 normalized by STAT6. (F) Photomicrographs illustrating immunofluorescence staining of Arginase-1, CD163, p-STAT6, respectively. (G–I) The count of Arginase-1, CD163, p-STAT6-positive cells. Data are expressed as mean ± SEM. **** P < 0.0001. N.S., statistically not significant, with the comparisons labeled. Scale bars in (F) = 50 μm.

Article Snippet: LCZ696 (S7678) was purchased from Selleckchem (Houston, TX, USA).

Techniques: Western Blot, Immunofluorescence, Staining, Labeling

Journal: Renal Failure

Article Title: LCZ696, an angiotensin receptor-neprilysin inhibitor, ameliorates epithelial-mesenchymal transition of peritoneal mesothelial cells and M2 macrophage polarization

doi: 10.1080/0886022X.2024.2392849

Figure Lengend Snippet: LCZ696 Inhibits EMT by blocking TGF-β1 secretion from M2 macrophages. (A) TGF-β1 level in culture medium of Raw264.7 cells from each group by ELISA kit detection. (B) Experimental scheme for cell treatment: Raw264.7 cells were treated with IL-4 and LCZ696 for 24 hours. Subsequently, the conditioned medium was harvested and used to incubate HPMCs for an additional 36 hours, after which further detections were conducted. (C) Western blot was conducted to evaluate the protein level of α-SMA, Collagen I, Collagen III, Fibronectin, E-cadherin and GAPDH in HPMCs cell lysates. (D–H) Scatter plots showing the densitometry analysis of α-SMA, Collagen I, Collagen III, Fibronectin and E-cadherin normalized by GAPDH. Data are expressed as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. N.S., statistically not significant, with the comparisons labeled.

Article Snippet: LCZ696 (S7678) was purchased from Selleckchem (Houston, TX, USA).

Techniques: Blocking Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Labeling