Journal: Clinical & Translational Immunology

Article Title: Naturally derived cytokine peptides limit virus replication and severe disease during influenza A virus infection

doi: 10.1002/cti2.1443

Figure Lengend Snippet: Human growth hormone‐derived LAT8881 improves in vitro cell survival in either a LANCL1 or LANCL2 protein‐dependent manner. (a) Schematic of human growth hormone structure (green, http://www.rcsb.org/structure/1HGU ). The synthetic compound LAT8881 comprises the short C‐terminal region (red) and is cyclised by a disulphide bond between two cysteine residues as shown. (b) Viability of murine L cells (1000 or 5000 cells) relative to vehicle control samples following 24‐ or 48‐h incubation with LAT8881 (200 μM) or vehicle (DMSO) alone, as determined by luminescent ATP detection ± SD. **** P < 0.0001 vs vehicle control, Student's t ‐test. Data are pooled from three independent experiments. (c) Viability of murine peritoneal exudate cells relative to media control in culture at 24 h following LAT8881 (10 μM) or vehicle (DMSO) alone, as determined by luminescent ATP detection ± SD. * P < 0.05, ** P < 0.01 vs media control, two‐way ANOVA. Data are pooled from two independent experiments. (d) Viability of human A549 cells 16 h following 350 μM paclitaxel or vehicle (DMSO) alone ± LAT8881 (1–100 μM), as determined by luminescent ATP detection ± SD. **** P < 0.0001 vs paclitaxel without LAT8881, two‐way ANOVA. Data are representative of two independent experiments. (e) Viability of murine L cells transfected with 100 nM siRNA against Lancl1 or Lancl2 relative to control siRNA samples at 72 or 120 h, as determined by luminescent ATP detection ± SD. * P < 0.05, *** P < 0.001, **** P < 0.0001 vs control siRNA, one‐way ANOVA. Data are pooled from three independent experiments. (f) Viability of human A549 cells transfected with 100 nM siRNA specific to LANCL1 , LANCL2 or control siRNA 16 h following 350 μM paclitaxel ± LAT8881 (1–100 μM) or vehicle, as determined by luminescent ATP detection ± SD. ** P < 0.001, **** P < 0.0001 vs paclitaxel + control siRNA without LAT8881, two‐way ANOVA. Data are representative of two independent experiments.

Article Snippet: In some experiments, L cells were transfected with 100 nM siRNA specific for mouse Lancl1 (Ambion In Vivo , Life Technologies, Carlsbad, USA, Cat #4457308), Lancl2 (Ambion In Vivo , Life Technologies, Carlsbad, USA, Cat #4457308) or control siRNA (Ambion In Vivo Negative Control #1; Cat #4457289) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, USA, Cat #L3000015).

Techniques: Derivative Assay, In Vitro, Control, Incubation, Transfection

Journal: Cells

Article Title: Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis

doi: 10.3390/cells11244135

Figure Lengend Snippet: Results of fertility disturbed by the LanCL1 gene.

Article Snippet: The anti-β-actin antibody (Cell Signaling Technology, Boston, MA, USA) and anti-LanCL1 antibody (Invitrogen, Carlsbad, CA, USA) were used to detect the protein expression according to the manufacturer’s protocol.

Techniques:

Journal: Cells

Article Title: Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis

doi: 10.3390/cells11244135

Figure Lengend Snippet: LanCL1 gene knockout influences the semen quality. ( a ) The expression of mRNA and protein in the LanCL1+/+, LanCL1+/− and LanCL1−/− mice. ( b ) Parameters of semen quality in the LanCL1+/+ and LanCL1−/− mice. ( c ) Sperm morphology.

Article Snippet: The anti-β-actin antibody (Cell Signaling Technology, Boston, MA, USA) and anti-LanCL1 antibody (Invitrogen, Carlsbad, CA, USA) were used to detect the protein expression according to the manufacturer’s protocol.

Techniques: Gene Knockout, Expressing

Journal: Cells

Article Title: Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis

doi: 10.3390/cells11244135

Figure Lengend Snippet: Single-cell RNAseq of the testis identified the high expression of the Cir1 gene in LanCL1−/− mice. ( a ) UAMP plot of the cell clusters. ( b ) Heatmap of the marker genes in the cell clusters. ( c ) The LanCL1 gene expression in the cell clusters. ( d ) Immunofluorescence of the LanCL1 gene in the testicular tissue. ( e ) The volcano plot of the DEGs in the LanCL1+/+ and LanCL1−/− mice. ( f ) Venn diagram of up-regulation in the Germ cells of the LanCL1−/− cell.

Article Snippet: The anti-β-actin antibody (Cell Signaling Technology, Boston, MA, USA) and anti-LanCL1 antibody (Invitrogen, Carlsbad, CA, USA) were used to detect the protein expression according to the manufacturer’s protocol.

Techniques: Expressing, Marker, Immunofluorescence

Journal: Cells

Article Title: Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis

doi: 10.3390/cells11244135

Figure Lengend Snippet: The LanCL1 gene maintains the development of normal sperm motility in germ cells. ( a ) The percent of cell numbers in every cell cluster from LanCL1+/+ and LanCL1−/− mice. ( b ) UAMP plot of the cluster 7. ( c ) The cell percent of new clusters. ( d ) GO annotation of the DEGs in the LanCL1−/− and LanCL1+/+ mice.

Article Snippet: The anti-β-actin antibody (Cell Signaling Technology, Boston, MA, USA) and anti-LanCL1 antibody (Invitrogen, Carlsbad, CA, USA) were used to detect the protein expression according to the manufacturer’s protocol.

Techniques:

Journal: Cells

Article Title: Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis

doi: 10.3390/cells11244135

Figure Lengend Snippet: The cell interactions signaling in various cell clusters regulated by the LanCL1 gene. ( a ) The cell communication before and after LanCL1 gene knockout. ( b ) The alteration of signaling pathway. ( c ) Heatmap of the active signaling pathway in every cell clusters. ( d ) PDGF signaling pathway. ( e ) GALECTIN signaling pathway. ( f ) The M1 and M2 macrophage marker genes.

Article Snippet: The anti-β-actin antibody (Cell Signaling Technology, Boston, MA, USA) and anti-LanCL1 antibody (Invitrogen, Carlsbad, CA, USA) were used to detect the protein expression according to the manufacturer’s protocol.

Techniques: Gene Knockout, Marker

Journal: Cells

Article Title: Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis

doi: 10.3390/cells11244135

Figure Lengend Snippet: LanCL1 gene regulated the P53 and PPAR signaling other than the testosterone synthesis in the Leyding cells. ( a ) The numbers of DEGs in every cell clusters. ( b ) The GSEA of the DEGs in the LanCL1 +/+ and LanCL1− / − mice of Leydig cell. ( c ) The steroid biosynthesis. ( d ) The marker genes of testosterone biosynthesis. ( e ) Serum testosterone level in the LanCL1 +/+ and LanCL1− / − mice.

Article Snippet: The anti-β-actin antibody (Cell Signaling Technology, Boston, MA, USA) and anti-LanCL1 antibody (Invitrogen, Carlsbad, CA, USA) were used to detect the protein expression according to the manufacturer’s protocol.

Techniques: Marker