Journal: Renal Failure

Article Title: Targeting LRH-1 alleviates diabetes-induced lipotoxicity in podocytes: role of PLIN5-mediated lipid droplet turnover

doi: 10.1080/0886022X.2026.2670806

Figure Lengend Snippet: LRH-1 overexpression alleviated HG-induced lipid accumulation and lipid droplet turnover. (A) ORO staining of cultured podocytes. HPCs were treated with HG for 24 hours. (B–D) Western blot assay showing expression of LRH-1 and PLIN5 among different treatment groups and quantitative analyses ( n = 6). (E) The relative MDA among different groups ( n = 6). (F) Quantitative analysis of podocytes FFA in each group ( n = 3). (G) Quantitative analysis of podocytes ROS levels in each group ( n = 6). (H) Representative images and quantification of MitoTracker (red), BODIPY (green), and DAPI (blue)-immunofluorescent stained podocytes from each group ( n = 6). (I) Representative images and quantification of LAMP1 (red), BODIPY (green), and DAPI (blue)-immunofluorescent stained podocytes from each group ( n = 6). * p < 0.05 and ** p < 0.01. Five visual fields for each section analyzed.

Article Snippet: Rabbit anti-LRH-1 antibodies and rabbit anti-PLIN5 antibodies were obtained from Proteintech (Wuhan, China); mouse anti-Wilms’ tumor 1 (WT1) antibodies were purchased from Novus (Centennial, CO); mouse anti-Lamp1 antibodies were purchased from Progen Biotechnik (Heidelberg, Germany); anti-β-actin mouse monoclonal antibody, fluorescent secondary antibodies were purchased from Antgene (Wuhan, China).

Techniques: Over Expression, Staining, Cell Culture, Western Blot, Expressing

Journal: eLife

Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

doi: 10.7554/eLife.101533

Figure Lengend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

Techniques: Expressing, Staining

Journal: eLife

Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

doi: 10.7554/eLife.101533

Figure Lengend Snippet: ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

Techniques: Expressing, Staining, Immunofluorescence, Marker