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probiotic strain l. paracasei cncm i-4034  (Pasteur Institute)
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Dendritic cells (DCs) were incubated with the <t>probiotic</t> L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. E. coli lipopolysaccharide (LPS, 20 ng/ml) was used as a positive control. Negative-control cultures contained unstimulated DCs. IL-1β, IL-6, IL-8, IL-12(p40) and IL-12(p70) production was measured. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.
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Dendritic cells (DCs) were incubated with the probiotic L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. E. coli lipopolysaccharide (LPS, 20 ng/ml) was used as a positive control. Negative-control cultures contained unstimulated DCs. IL-1β, IL-6, IL-8, IL-12(p40) and IL-12(p70) production was measured. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: Dendritic cells (DCs) were incubated with the probiotic L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. E. coli lipopolysaccharide (LPS, 20 ng/ml) was used as a positive control. Negative-control cultures contained unstimulated DCs. IL-1β, IL-6, IL-8, IL-12(p40) and IL-12(p70) production was measured. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Incubation, Positive Control, Negative Control

DCs were incubated with the probiotic L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. IL-10, TNF-α, TGF-β1 and TGF-β2 production was measured. LPS, 20 ng/ml, was used as a positive control. Negative-control cultures contained unstimulated DCs. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi ; N.D indicate no detected.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: DCs were incubated with the probiotic L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. IL-10, TNF-α, TGF-β1 and TGF-β2 production was measured. LPS, 20 ng/ml, was used as a positive control. Negative-control cultures contained unstimulated DCs. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi ; N.D indicate no detected.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Incubation, Positive Control, Negative Control

DCs were incubated with the probiotic L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. The production of IFNγ and the chemokines MCP-1/CCL2, MIP-1α/CCL3, RANTES/CCL5 and MDC/CCL22 was measured. LPS, 20 ng/ml, was used as a positive control. Negative-control cultures contained unstimulated DCs. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: DCs were incubated with the probiotic L. paracasei CNCM I-4034 (Prob) or its cell-free supernatant (CFS), Salmonella ( S.typhi ), or both. The production of IFNγ and the chemokines MCP-1/CCL2, MIP-1α/CCL3, RANTES/CCL5 and MDC/CCL22 was measured. LPS, 20 ng/ml, was used as a positive control. Negative-control cultures contained unstimulated DCs. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Incubation, Positive Control, Negative Control

Comparison of the expression of TLR1, TLR2, TLR3, TLR4 and TLR5 in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The data shown are the mean values and SEM of three independent experiments. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. *p<0.05 compared to controls; #p<0.05 compared to S.typhi. N.D indicate no detected.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: Comparison of the expression of TLR1, TLR2, TLR3, TLR4 and TLR5 in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The data shown are the mean values and SEM of three independent experiments. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. *p<0.05 compared to controls; #p<0.05 compared to S.typhi. N.D indicate no detected.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Comparison, Expressing, Positive Control, Control

Comparison of the expression of TLR9, MYD88, IRAK-1, IRAK-4 and TOLLIP in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: Comparison of the expression of TLR9, MYD88, IRAK-1, IRAK-4 and TOLLIP in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Comparison, Expressing, Positive Control, Control

Comparison of the expression of CASP8, TAK-1, JNK, IRF-3 and MAPK14 in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: Comparison of the expression of CASP8, TAK-1, JNK, IRF-3 and MAPK14 in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Comparison, Expressing, Positive Control, Control

Comparison of the expression of NFKBIA, NFKB-1, TBK-1, IL-10 and TNF- α in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi. N.D indicate no detected.

Journal: PLoS ONE

Article Title: Human Intestinal Dendritic Cells Decrease Cytokine Release against Salmonella Infection in the Presence of Lactobacillus paracasei upon TLR Activation

doi: 10.1371/journal.pone.0043197

Figure Lengend Snippet: Comparison of the expression of NFKBIA, NFKB-1, TBK-1, IL-10 and TNF- α in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella ( S.typhi ) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments. *p<0.05 compared to controls; #p<0.05 compared to S.typhi. N.D indicate no detected.

Article Snippet: The probiotic strain (designated by the Pasteur Institute as L. paracasei CNCM I-4034) was isolated from the faeces of breast-fed newborn children.

Techniques: Comparison, Expressing, Positive Control, Control