Journal: iScience

Article Title: KRT15 identified by scRNA-Seq and machine learning as stemness regulator and prognostic biomarker in ESCC

doi: 10.1016/j.isci.2026.115020

Figure Lengend Snippet: KRT15 is enriched in ESCC tumor spheroids and positively correlates with the stemness marker CD44 (A) Stem cell enrichment experiments performed in four cell lines, with images captured over time. (B and C) qPCR analysis of CD44 and KRT15 mRNA expression in tumorspheres versus adherent cells from ECA109, KYSE180, KYSE150, and TE1 cell lines. n = 3, statistical analysis performed using an unpaired t test. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (D) Western blot analysis of CD44 and KRT15 protein expression in tumor spheres compared to adherent cells in ECA109 and KYSE180 cell lines.

Article Snippet: Human: KYSE150 cells , DSMZ , ACC-375.

Techniques: Marker, Expressing, Western Blot

Journal: Scientific Reports

Article Title: Overexpression of LINC01354 predicts well prognosis and suppresses esophageal cancer progression

doi: 10.1038/s41598-025-20455-2

Figure Lengend Snippet: High tumor expression of LINC01354 holds diagnostic potential in ESCA. ( A ) A comparative analysis of LINC01354 expression and prognosis revealed that LINC01354 was decreased in ESCA and BRCA and led to poor prognosis in patients. ( B , C ) The high expression of LINC01354 in ESCA was validated in additional two cohorts. ( D ) Paired analysis showed that LINC01354 was down-regulated in 95% of ESCA patients in GSE53622 cohort. ( E ) Further analysis depicted that LINC01354 was decreased in 89.9% of ESCA patients in GSE53624 cohort. ( F , G ) ROC analysis was used to evaluate the diagnostic potential of LINC01354 in the GSE53622 (95% confidence interval: 0.8317 to 0.9544) and GSE53624 (95% confidence interval: 0.8374 to 0.9277) cohorts. ( H ) The expression of LINC01354 in Het-1 A, TE1, KYSE70, KYSE150, and KYSE450 cells was examined by qRT-PCR, ** p < 0.01, *** p < 0.001, independent-samples T test. ( I ) FISH was employed to detect the expression of LINC01354 in ESCA TMA, scale bar = 800 μm.

Article Snippet: Human ESCA cell lines KYSE150 and TE1 were purchased from Procell (Wuhan, China).

Techniques: Expressing, Diagnostic Assay, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Overexpression of LINC01354 predicts well prognosis and suppresses esophageal cancer progression

doi: 10.1038/s41598-025-20455-2

Figure Lengend Snippet: LINC01354 is involved in cell-cycle regulation and curbs tumorigenesis in ESCA. ( A , B ) Functional enrichment analysis of LINC01354 associated genes in ESCA revealed the biological function of LINC01354. ( C – E ) The effect of overexpression of LINC01354 on ESCA tumor growth was evaluated in a nude mouse model, ** p < 0.01. ( F , G ) The inhibition of LINC01354 on KYSE150 cell cycle process was validated by flow cytometry analysis, **** p < 0.0001.

Article Snippet: Human ESCA cell lines KYSE150 and TE1 were purchased from Procell (Wuhan, China).

Techniques: Functional Assay, Over Expression, Inhibition, Flow Cytometry