Journal: Cell & Bioscience

Article Title: Multi-omics analysis unveiled fibroblast-mediated pathogenesis in male genital lichen sclerosus

doi: 10.1186/s13578-025-01453-3

Figure Lengend Snippet: Sub-cluster analysis of keratinocytes delineated a unique subtype in interaction with fibroblasts via the APP-CD74 pathway. A .The sub-clustering of keratinocytes among the three groups. B .The dot plot of the expression of the epithelial markers in the keratinocyte sub-clusters. C .The subtype annotation of keratinocytes among the three groups. D .The proportion analysis of the annotated keratinocyte subtypes and sample groups. E .The expression profile of CD74 among the keratinocyte subtypes of the three groups. F .The mIHC images demonstrated a robust interaction (APP-CD74) between the fibroblasts (COL6A1+) and the keratinocytes (KRT15+). Scale bar: 40 μm. mIHC: multiplex immunohistochemistry

Article Snippet: The primary antibodies included: ELN (1:50 dilution, Cat# sc-58756, Santa Cruz Biotechnology, Inc.); COL1A1 (1:200 dilution, Cat# ab34710, Abcam plc.); COL6A1 (1:100 dilution, Cat# A9738, ABclonal Technology Co., Ltd.); CD44 (1:100 dilution, Cat# 37259, Cell Signaling Technology, Inc.); CD3E (1:150 dilution, Cat# ab16669, Abcam plc.); LAG3 (1:200 dilution, Cat# 15372, Cell Signaling Technology, Inc.); APP (1:100 dilution, Cat# 193895, Cell Signaling Technology, Inc.); CD74 (1:250 dilution, Cat# 772745, Cell Signaling Technology, Inc.); and KRT15 (1:200 dilution, Cat# 60247-1-Ig, Proteintech Group, Inc.).

Techniques: Expressing, Multiplex Assay, Immunohistochemistry