Journal: American Journal of Translational Research

Article Title: FCGBP promotes malignant phenotypes of glioblastoma cells via activation of the TGF-β1 signaling pathway

doi: 10.62347/NVYV1514

Figure Lengend Snippet: FCGBP knockdown suppressed the growth of subcutaneous U87 xenograft tumors in nude mice. A. Representative images of subcutaneous tumors from nude mice of the sh-NC and sh-FCGBP groups. B. Comparison of terminal tumor weight. C. Changes in tumor volume over time. D. Body weight changes in mice. E. Hematoxylin and eosin (H&E) staining of tumor tissues and quantitative analysis of tumor necrosis score, magnification: ×200. F. ELISA analysis of serum levels of TGF-β, TNF-α, IL-6, and IL-1β. G. IHC detection of Ki-67 and PCNA expression in tumor tissues, magnification: ×200. H. TUNEL staining and quantitative analysis of TUNEL-positive signal intensity per unit area in tumor tissues, magnification: ×200. Data are presented as mean ± SD from three independent experiments. Tumor volume and body weight curves were analyzed using two-way repeated-measures ANOVA followed by Bonferroni’s post-hoc test. Other two-group comparisons were analyzed using an unpaired Student’s t-test. Compared with the sh-NC group: *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Sections were blocked and incubated overnight with primary antibodies against Ki-67 (1:1,000, Proteintech, 28074-1-AP) and PCNA (1:500, Proteintech, 10205-2-AP).

Techniques: Knockdown, Xenograft Assay, Comparison, Staining, Analysis, Enzyme-linked Immunosorbent Assay, Expressing, TUNEL Assay