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NOV340 liposomes deliver RNA into AML cells in vitro via macropinocytosis (A) Graphic of Cy3-MTL-CEBPA and controls. (B) Delivery of fluorescently tagged CEBPA-51 saRNA in myeloid leukemia and lymphoid leukemia cell lines. Two-way ANOVA with Holm-Sidak multiple comparison test; n = 3. Data represented as mean (SD). (C) Schematic of endocytosis inhibitors used for in vitro uptake inhibition assay. (D) Representative histograms of Cy3 fluorescence in AML cell lines after treatment with clathrin-mediated endocytosis inhibitor, Pitstop 2, or macropinocytosis inhibitors, EIPA or imipramine. (E) Changes in uptake of Cy3-MTL-CEBPA following treatment with endocytosis inhibitors in THP-1, MOLM-14, and <t>KG1a</t> cells. Two-way ANOVA with Holm-Sidak multiple comparison test; n = 3. Data represented as mean (SD).
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NOV340 liposomes deliver RNA into AML cells in vitro via macropinocytosis (A) Graphic of Cy3-MTL-CEBPA and controls. (B) Delivery of fluorescently tagged CEBPA-51 saRNA in myeloid leukemia and lymphoid leukemia cell lines. Two-way ANOVA with Holm-Sidak multiple comparison test; n = 3. Data represented as mean (SD). (C) Schematic of endocytosis inhibitors used for in vitro uptake inhibition assay. (D) Representative histograms of Cy3 fluorescence in AML cell lines after treatment with clathrin-mediated endocytosis inhibitor, Pitstop 2, or macropinocytosis inhibitors, EIPA or imipramine. (E) Changes in uptake of Cy3-MTL-CEBPA following treatment with endocytosis inhibitors in THP-1, MOLM-14, and KG1a cells. Two-way ANOVA with Holm-Sidak multiple comparison test; n = 3. Data represented as mean (SD).

Journal: Molecular Therapy. Nucleic Acids

Article Title: RNA activation of CEBPA improves leukemia treatment

doi: 10.1016/j.omtn.2025.102611

Figure Lengend Snippet: NOV340 liposomes deliver RNA into AML cells in vitro via macropinocytosis (A) Graphic of Cy3-MTL-CEBPA and controls. (B) Delivery of fluorescently tagged CEBPA-51 saRNA in myeloid leukemia and lymphoid leukemia cell lines. Two-way ANOVA with Holm-Sidak multiple comparison test; n = 3. Data represented as mean (SD). (C) Schematic of endocytosis inhibitors used for in vitro uptake inhibition assay. (D) Representative histograms of Cy3 fluorescence in AML cell lines after treatment with clathrin-mediated endocytosis inhibitor, Pitstop 2, or macropinocytosis inhibitors, EIPA or imipramine. (E) Changes in uptake of Cy3-MTL-CEBPA following treatment with endocytosis inhibitors in THP-1, MOLM-14, and KG1a cells. Two-way ANOVA with Holm-Sidak multiple comparison test; n = 3. Data represented as mean (SD).

Article Snippet: THP-1, HL-60, K562, and KG1a cell lines were obtained from the American Type Culture Collection (ATCC).

Techniques: Liposomes, In Vitro, Comparison, Inhibition, Fluorescence

MTL-CEBPA upregulates CEBPA in AML in vitro models (A) Changes in CEBPA mRNA expression in THP-1, KG1a, or MOLM-14 cell lines 72-h post-MTL-CEBPA or control (MTL-FLUC) treatment. Two-tailed unpaired student t tests; n = 3. Data represented as mean (SD). (B) Representative western blots of THP-1 and MOLM-14 cell lines 96 h post-treatment. (C) Upregulation of CEBPA mRNA in ex vivo AML3 patient sample 48 h post MTL-CEBPA or control (MTL-FLUC) treatment. Two-tailed unpaired student t test; n = 3. Data represented as mean (SD).

Journal: Molecular Therapy. Nucleic Acids

Article Title: RNA activation of CEBPA improves leukemia treatment

doi: 10.1016/j.omtn.2025.102611

Figure Lengend Snippet: MTL-CEBPA upregulates CEBPA in AML in vitro models (A) Changes in CEBPA mRNA expression in THP-1, KG1a, or MOLM-14 cell lines 72-h post-MTL-CEBPA or control (MTL-FLUC) treatment. Two-tailed unpaired student t tests; n = 3. Data represented as mean (SD). (B) Representative western blots of THP-1 and MOLM-14 cell lines 96 h post-treatment. (C) Upregulation of CEBPA mRNA in ex vivo AML3 patient sample 48 h post MTL-CEBPA or control (MTL-FLUC) treatment. Two-tailed unpaired student t test; n = 3. Data represented as mean (SD).

Article Snippet: THP-1, HL-60, K562, and KG1a cell lines were obtained from the American Type Culture Collection (ATCC).

Techniques: In Vitro, Expressing, Control, Two Tailed Test, Western Blot, Ex Vivo