Journal: Frontiers in Bioengineering and Biotechnology

Article Title: αvβ3-targeted gas vesicles for ultrasound molecular imaging of tumors

doi: 10.3389/fbioe.2026.1808539

Figure Lengend Snippet: In vitro cell targeting studies. (A–C) Flow cytometry assay of bEnd.3 cells, K7M2 cells and B16-F10 cells incubated with FITC-Con, FITC-RGD or free RGD + FITC-RGD, respectively. Representative fluorescent microscope images of bEnd.3 cells (D) , K7M2 cells (F) and B16-F10 cells (H) incubated with FITC-Con-hGVs, FITC-RGD-hGVs or free RGD + FITC-RGD-hGVs. Green stands for FITC, and blue for cell nuclei stained with DAPI. Scale bar: 50 µm. (E,G,I) represent the quantitative analysis of the fluorescence intensity in (D,F,H) , respectively. *** for P < 0.001; **** for P < 0.0001.

Article Snippet: The K7M2 and B16-F10 cell lines, as well as mouse bEnd.3 endothelial cells, were obtained from ATCC.

Techniques: In Vitro, Flow Cytometry, Incubation, Microscopy, Staining, Fluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: αvβ3-targeted gas vesicles for ultrasound molecular imaging of tumors

doi: 10.3389/fbioe.2026.1808539

Figure Lengend Snippet: In vivo ultrasound molecular imaging of tumors. (A) Nonlinear contrast images of Con-hGVs and RGD-hGVs were obtained at various time points after intravenous injection in K7M2 osteosarcoma and B16-F10 melanoma tumor-bearing mice. Time–intensity curves of Con-hGVs and RGD-hGVs after intravenous injection in K7M2 osteosarcoma–bearing mice (B) , and corresponding tumor signal intensities at 1, 3, 5, 7, and 10 min post-injection (C) . Time–intensity curves of Con-hGVs and RGD-hGVs after intravenous injection in B16-F10 tumor–bearing mice (D) , and corresponding tumor signal intensities at 1, 3, 5, 7, and 10 min post-injection (E) .

Article Snippet: The K7M2 and B16-F10 cell lines, as well as mouse bEnd.3 endothelial cells, were obtained from ATCC.

Techniques: In Vivo, Imaging, Injection

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: αvβ3-targeted gas vesicles for ultrasound molecular imaging of tumors

doi: 10.3389/fbioe.2026.1808539

Figure Lengend Snippet: Immunofluorescence and quantitative analysis of tumors. (A,B) Vascular immunofluorescence images and their quantification were obtained 600 s following the intravenous injection of FITC-labeled Con-hGVs or RGD-hGVs in K7M2 osteosarcoma-bearing mice. (C) The corresponding 3D surface plot graphs are presented in. (D,E) Vascular immunofluorescence images and quantification were acquired 600 s following the intravenous injection of FITC-labeled Con-hGVs or RGD-hGVs in B16-F10 melanoma-bearing mice, with the corresponding 3D surface plot graphs displayed in (F) .

Article Snippet: The K7M2 and B16-F10 cell lines, as well as mouse bEnd.3 endothelial cells, were obtained from ATCC.

Techniques: Immunofluorescence, Injection, Labeling

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: αvβ3-targeted gas vesicles for ultrasound molecular imaging of tumors

doi: 10.3389/fbioe.2026.1808539

Figure Lengend Snippet: Biosafety analysis. (A) Hemolysis testing of RGD-hGVs at OD 500 = 0.5, 1.0, 1.5, 2.0, 2.5, or 3.0, using Triton X-100 as the positive control and PBS as the negative control (n = 3). (B) Viability of bEnd.3, K7M2, and B16-F10 cells following exposure to RGD-hGVs at OD 500 = 0.5, 1.0, 1.5, 2.0, 2.5, or 3.0 for 6 h (n = 3). (C) H&E histology of major organs from mice administered Con-hGVs (OD 500 = 3.0), RGD-hGVs (OD 500 = 3.0), or PBS; scale bar, 200 μm. (D–G) Serum markers of hepatic function and renal function measured 7 days after injection of PBS, Con-hGVs, or RGD-hGVs (n = 3).

Article Snippet: The K7M2 and B16-F10 cell lines, as well as mouse bEnd.3 endothelial cells, were obtained from ATCC.

Techniques: Positive Control, Negative Control, Injection

Journal: Frontiers in Immunology

Article Title: Neutrophil elastase promotes low molecular weight cyclin E1 formation to accelerate osteosarcoma proliferation

doi: 10.3389/fimmu.2025.1647913

Figure Lengend Snippet: Osteosarcoma neutrophil elastase (ELA2) promotes osteosarcoma cell proliferation in vitro and in vivo . (A) Colony formation assay showed that ELA2 accelerated osteosarcoma proliferation in U2OS and 143B cells (unpaired two-tailed Student’s t -test, N = 3). (B) Immunofluorescence image showed that Lv-ELA2 was successfully transfected into K7M2 cell lines and stably expressed (scale bar: 100 μm). (C) Quantitative analysis of relative ELA2 expression level in the Lv-Ctrl group and Lv-ELA2 group by qPCR assays (unpaired two-tailed Student’s t -test, N = 3). (D) The results of the cell cycle in the Ctrl group and ELA2-OE group using flow cytometry (left). The statistical analysis of G0/G1 phase, S phase, and G2/M phase cell proportions (right) (unpaired two-tailed Student’s t -test, N = 3). (E) Images of tumors from the Ctrl group and ELA2-OE group tumors ( N = 3 for each group). (F) Tumor weight and tumor volume were measured and quantitatively analyzed (tumor volume = length × width 2 /2; unpaired two-tailed Student’s t -test, N = 3). (G) Images of Ki-67 + immunohistochemistry staining in the Ctrl group and ELA2-OE group (scale bar: 100 μm). ( *** p < 0.001).

Article Snippet: 143B (CVCL_2270), KHOS (CVCL_2546), HOS (CVCL_0312), MG63 (CVCL_0426), U2OS (CVCL_0042), and SaoS2 (CVCL_0548) cells were human osteosarcoma cell lines, and K7M2 cells were mouse osteosarcoma cell lines; all were purchased from the American Type Culture Collection.

Techniques: In Vitro, In Vivo, Colony Assay, Two Tailed Test, Immunofluorescence, Transfection, Stable Transfection, Expressing, Flow Cytometry, Immunohistochemistry, Staining