Journal: The EMBO Journal

Article Title: The TPR2 corepressor forms condensates with repressors to fine-tune growth and development in rice

doi: 10.1038/s44318-026-00852-7

Figure Lengend Snippet: ( A ) Volcano plot showing differentially expressed genes (DEGs) between WT and tpr2 (left, n = 2 biological replicates per group); and between COM-FL and tpr2 (right, n = 2 biological replicates per group). Differential expression analysis was performed using DESeq2 based on a negative binomial generalized linear model with Benjamini–Hochberg false discovery rate (FDR) correction for multiple testing. Genes with |log₂ (fold change)|>0.5 and adjusted p < 0.05 were considered significantly differentially expressed (red: upregulated; blue: downregulated; gray: not significant). ( B ) Venn diagram showing the overlap of downregulated DEGs in WT vs tpr2 (3987 DEGs) and COM-FL vs tpr2 (2088 DEGs). 837 DEGs were common to both comparisons. ( C ) Heatmap of 837 overlapping DEGs across WT, tpr2 , COM-FL, COM-ΔIDR1, COM-M6, and COM-M8 plants. Gene expression levels are shown as FPKM values (red, high; blue, low). ( D ) Immunoblot analysis of histone acetylation levels in WT and tpr2 seedlings. H3 acetylation was detected with anti-H3ac, anti-H3K9ac, anti-H3K14ac, anti-H3K18ac, anti-H3K23ac and anti-H3K27ac antibodies. Total H3 was used as a loading control. Experiments were independently repeated at least three times with similar results. ( E ) Immunoblot analysis of H3K9 acetylation levels in WT, tpr2 , COM-ΔIDR1, COM-M6 and COM-M8 lines. Histone extracts from WT, tpr2 , COM-ΔIDR1, COM-M6, and COM-M8 seedlings were subjected to immunoblotting using anti-acetyl-H3K9 antibody. Total H3 was used as a loading control. Experiments were independently repeated at least three times with similar results. ( F ) Density distribution of H3K9ac signals across the gene body of the 837 DEGs. Genes are grouped by genotype: tpr2 , COM-FL, COM-ΔIDR1, COM-M6, and COM-M8. TSS, transcription start site; TES, transcription end site. ( G ) Heatmap of H3K9ac CUT&Tag signals upstream and downstream of the gene body. Regions include 3000 bp upstream of TSS and 3000 bp downstream of TES. Data were representative of two independent experiments. .

Article Snippet: anti-H3ac , ABclonal , Cat#A17917.

Techniques: Quantitative Proteomics, Gene Expression, Western Blot, Control

Journal: JID Innovations

Article Title: Zinc flux in skin: Identity and function of metallothionein 1–positive cells in skin homeostasis

doi: 10.1016/j.xjidi.2026.100483

Figure Lengend Snippet: Single-cell transcriptomic profiling of Mt1 + epidermal cells. ( a ) UMAP visualization of 12 clusters (i) and feature plot of Mt1 expression (ii), showing enrichment in cluster 3. ( b ) Feature plots showing the expression of (i) Mt1 , (ii) Trp63 , (iii) Itga6 , (iv) K14 , and (v) K15 across 12 clusters identified through single-cell RNA-sequencing analysis. ( c ) Feature plots showing limited overlap between Mt1 + cells and classical epidermal stem cell markers, including (i) Lrig1 , (ii) Prdm1 , (iii) Lgr5 , (iv) Lgr6 , (v) Gli1 , and (vi) Cd34 . ( d ) Histological cut showing the expression of K14 , accessed from The Human Protein Atlas. Bar = 200 μm. ( e ) Volcano plot showing differentially expressed genes in Mt1- high (Mt1 > 3) versus Mt1- negative (Mt1 = 0) cells. Selected upregulated and downregulated genes are highlighted. ( f ) Gene ontology/pathway enrichment analysis of differentially expressed genes in Mt1- high versus Mt1- negative cells. UMAP, Uniform Manifold Approximation and Projection.

Article Snippet: Single-cell transcriptomic profiling of Mt1 + epidermal cells. ( a ) UMAP visualization of 12 clusters (i) and feature plot of Mt1 expression (ii), showing enrichment in cluster 3. ( b ) Feature plots showing the expression of (i) Mt1 , (ii) Trp63 , (iii) Itga6 , (iv) K14 , and (v) K15 across 12 clusters identified through single-cell RNA-sequencing analysis. ( c ) Feature plots showing limited overlap between Mt1 + cells and classical epidermal stem cell markers, including (i) Lrig1 , (ii) Prdm1 , (iii) Lgr5 , (iv) Lgr6 , (v) Gli1 , and (vi) Cd34 . ( d ) Histological cut showing the expression of K14 , accessed from The Human Protein Atlas.

Techniques: Single Cell, Expressing, RNA Sequencing

Journal: bioRxiv

Article Title: RUNX1-ETO expression in epidermal keratinocytes induces progressive skin inflammation in vivo

doi: 10.64898/2026.05.10.724156

Figure Lengend Snippet: (a) Schematic representation of the inducible RUNX1-ETO/K14-CreER construct used for inducible expression in epidermal keratinocytes. (b) Experimental timeline. Control or RUNX1-ETO mice received a single intraperitoneal injection of tamoxifen (TM; 100 µg/g body weight) at 2 weeks of age. Mice were sacrificed at 1, 2, 3, 4, and 6 weeks post-TM. (c) Representative gross appearance of control and RUNX1-ETO mice following TM administration. Insets show higher-magnification views of inflamed regions outlined by black dashed boxes. (d) Time course of the proportion of mice exhibiting visible skin inflammation in control or RUNX1-ETO/K14-CreER mice. (e) Representative images of spleens from control and RUNX1-ETO mice and quantification of spleen-to-body weight ratios. Scale bar: 5 mm. (f) White blood cell (WBC) counts in the peripheral blood of control and RUNX1-ETO mice. Data are presented as the mean ± SD. Each dot represents one mouse. Significance was assessed using a two-tailed unpaired Student’s t test. * p < 0.05; ** p < 0.01; **** p < 0.0001; ns, not significant.

Article Snippet: RUNX1-ETO mice were crossed with K14-CreER mice (The Jackson Laboratory, strain 005107; C57BL/6J background) to obtain double transgenic mice.

Techniques: Construct, Expressing, Control, Injection, Two Tailed Test

Journal: Stem Cell Research & Therapy

Article Title: Interleukins decrease epithelial protein and gene expression by K14 + CD105+ equine hoof progenitor cells

doi: 10.1186/s13287-026-05013-5

Figure Lengend Snippet: Study design schematic. The isolation and characterization of equine SI K14 + CD105+ progenitor cells and the evaluation of environmental conditions on cell gene and protein expression are depicted

Article Snippet: Slides were then incubated with K14 (monoclonal, mouse anti-human, 0.2 mg/ml, # MA5-11599, Fisher Scientific, Rockford, IL) and CD105 (polyclonal, rabbit anti-human, 0.2 mg/ml, #PA5-16895, Invitrogen, Rockford, IL) primary antibodies for 12 h at 4 o C and rinsed with tris-buffered saline (TBS) containing 0.025% triton followed by incubation with labeled secondary antibodies (Alexa FluorTM 488, polyclonal, donkey anti-mouse, 2 mg/ml, #A-21202; Alexa FluorTM 594, chicken anti-rabbit, 2 mg/ml, #A21442, Thermo Fisher Scientific, Eugene, Oregon) for 2 h at room temperature protected from light.

Techniques: Isolation, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Interleukins decrease epithelial protein and gene expression by K14 + CD105+ equine hoof progenitor cells

doi: 10.1186/s13287-026-05013-5

Figure Lengend Snippet: Double positive cell percentages at multiple passages and localization of K14 and CD105 antigens in equine hoof stratum internum tissue and progenitor cell isolates. The majority of cells in primary cell isolates from healthy and fibrous stratum internum are K14 + CD105+ ( A ), and basal cells of the secondary epidermal lamella express both antigens ( B-D ). The double positive phenotype is maintained after cryopreservation, revitalization and culture ( E-H ). Percentages (mean ± SEM) of K14 + CD105+ cells from healthy and fibrous stratum internum tissue at cell passages (P) 0–2 ( A ). Columns with different superscripts within passages are significantly different ( p < 0.05). Fluorescent photomicrographs of healthy equine stratum internum sections labeled with antibodies against K14 (arrow, green, B ) or CD105 (arrow, red, C ) and merged images (arrow, yellow, D ). Scale bar, 27 μm. PEL = primary epidermal laminae; SEL = secondary epidermal laminae. Fluorescent photomicrographs of revitalized P3 K14 + CD105+ progenitor cells from healthy stratum internum labeled with antibodies against K14 (green, E ) or CD105 (red, F ) antigens separately and in a merged image (yellow, G ). Cells with nuclear stain only ( H , DAPI, blue). Scale bar, 100 μm

Article Snippet: Slides were then incubated with K14 (monoclonal, mouse anti-human, 0.2 mg/ml, # MA5-11599, Fisher Scientific, Rockford, IL) and CD105 (polyclonal, rabbit anti-human, 0.2 mg/ml, #PA5-16895, Invitrogen, Rockford, IL) primary antibodies for 12 h at 4 o C and rinsed with tris-buffered saline (TBS) containing 0.025% triton followed by incubation with labeled secondary antibodies (Alexa FluorTM 488, polyclonal, donkey anti-mouse, 2 mg/ml, #A-21202; Alexa FluorTM 594, chicken anti-rabbit, 2 mg/ml, #A21442, Thermo Fisher Scientific, Eugene, Oregon) for 2 h at room temperature protected from light.

Techniques: Labeling, Staining

Journal: Stem Cell Research & Therapy

Article Title: Interleukins decrease epithelial protein and gene expression by K14 + CD105+ equine hoof progenitor cells

doi: 10.1186/s13287-026-05013-5

Figure Lengend Snippet: Evaluation of heterogenous and K14 + CD105+ cell isolate E- and N- cadherin antigen and mesenchymal, EMT, and epidermal gene expression. Percentages of cells expressing E-cadherin and N-cadherin antigens vary among continuously cultured P3 heterogeneous and K14 + CD105+ isolates and within the K14 + CD105+ immunophenotype, respectively. Gene expression is similar between isolates except for K1 which is significantly higher in heterogeneous cells. Percentage (mean ± SEM) of cells expressing E- or N-cadherin in continuously cultured P3 heterogeneous and K14 + CD105+ equine hoof stratum internum progenitor cell isolates ( A ) and levels of mesenchymal, EMT, and epithelial gene expression (ΔCT) in the same cell isolates. Columns with different symbols are significantly different between cell isolates for a given cadherin, and columns with different cell superscripts are significantly different between cadherins within a cell isolate ( A ) or between cell isolates for a given gene ( B , p < 0.05). Reference gene: GAPDH. CD = cluster of differentiation; EMT = epithelial to mesenchymal transition; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; K = keratin; TGF = Transforming growth factor

Article Snippet: Slides were then incubated with K14 (monoclonal, mouse anti-human, 0.2 mg/ml, # MA5-11599, Fisher Scientific, Rockford, IL) and CD105 (polyclonal, rabbit anti-human, 0.2 mg/ml, #PA5-16895, Invitrogen, Rockford, IL) primary antibodies for 12 h at 4 o C and rinsed with tris-buffered saline (TBS) containing 0.025% triton followed by incubation with labeled secondary antibodies (Alexa FluorTM 488, polyclonal, donkey anti-mouse, 2 mg/ml, #A-21202; Alexa FluorTM 594, chicken anti-rabbit, 2 mg/ml, #A21442, Thermo Fisher Scientific, Eugene, Oregon) for 2 h at room temperature protected from light.

Techniques: Gene Expression, Expressing, Cell Culture

Journal: Stem Cell Research & Therapy

Article Title: Interleukins decrease epithelial protein and gene expression by K14 + CD105+ equine hoof progenitor cells

doi: 10.1186/s13287-026-05013-5

Figure Lengend Snippet: Assessment of the effects of interleukins and healthy matrix on TGF-β1 medium levels, cell E-cadherin and K14 antigen expression and epithelial and EMT gene expression. TGF-β1 medium levels are highest in cultures of K14 + CD105+ cells in stromal medium. Culture in stromal medium containing interleukins decreases the percentage of P3 K14 + CD105+ E-cadherin expressing cells while culture on healthy stratum internum matrix increases the percentage. Revitalized P3 K14 + CD105+ cell culture in stromal medium containing interleukins 2 and 6 decreases epithelial gene expression, and culture on healthy matrix in stromal medium increases epithelial gene expression and decreases EMT gene expression. Culture medium TGF- β1 levels ( A , mean ± SEM) in the medium, percentages of E-cadherin + and K14 + cells (B, mean ± SEM) and expression levels of TGF- β1, -2, -3, β-catenin, K1, K10, and p63 ( C , ΔCT, mean ± SEM) of revitalized P3 K14 + CD105+ cells from healthy stratum internum tissue after 21 days of culture in stromal medium alone (stromal) or with interleukins 2 and 6 (IL medium) or on decellularized SI matrix (healthy matrix). Reference gene: GAPDH. Significant differences among culture conditions are indicated by different superscripts above columns ( A ) and for each antigen ( B ) and gene (C, p < 0.05). CD = cluster of differentiation; EMT = epithelial to mesenchymal transition; GAPDH = glyceraldehyde-3-phosphate dehydrogenase; K = keratin; TGF = Transforming growth factor

Article Snippet: Slides were then incubated with K14 (monoclonal, mouse anti-human, 0.2 mg/ml, # MA5-11599, Fisher Scientific, Rockford, IL) and CD105 (polyclonal, rabbit anti-human, 0.2 mg/ml, #PA5-16895, Invitrogen, Rockford, IL) primary antibodies for 12 h at 4 o C and rinsed with tris-buffered saline (TBS) containing 0.025% triton followed by incubation with labeled secondary antibodies (Alexa FluorTM 488, polyclonal, donkey anti-mouse, 2 mg/ml, #A-21202; Alexa FluorTM 594, chicken anti-rabbit, 2 mg/ml, #A21442, Thermo Fisher Scientific, Eugene, Oregon) for 2 h at room temperature protected from light.

Techniques: Expressing, Gene Expression, Cell Culture

Journal: Acta Pharmaceutica Sinica. B

Article Title: FTO-mediated m 6 A depletion drives prenatal prednisone exposure-induced cortical neurogenesis deficits and long-term anxiety-depression-like behaviors in offspring

doi: 10.1016/j.apsb.2026.01.026

Figure Lengend Snippet: N 6 -Methyladenosine (m 6 A) regulated cell cycle and neuronal differentiation gene expression in PPE offspring rat RGCs histone 3 lysine 27 acetylation (H3K27ac) modification. (A) Dot blot analysis of m 6 A modification in total RNA from GD17 fetal rat cortices. (B) Volcano plot showing significantly altered mRNA expression. (C) Heatmap displaying the top 50 genes with significantly altered mRNA expression. (D) Gene ontology (GO) enrichment analysis of changed mRNAs. (E) Chord diagram illustrating the enrichment of differentially altered genes in the top 10 GO pathways. (F), (G) Real-time quantitative PCR (RT-qPCR) analysis of differential genes related to cell cycle and neuronal differentiation, n = 8. (H) Volcano plot showing differential methylation peaks. (I) GO enrichment analysis of histone modification-associated differential methylation peaks. (J) Representative Western blot images and quantification of H3K27ac, histone 3 lysine 14 acetylation (H3K14ac), and histone 3 lysine 9 acetylation (H3K9ac) levels in nuclear proteins, n = 3. (K), (L) Chromatin immunoprecipitation (ChIP)-qPCR assay showing the expression of H3K27ac and H3K14ac at the promoters and enhancers of cell cycle and neuronal differentiation-related differential genes, n = 6. (M) Representative Western blot images and quantification of nuclear protein levels of cAMP response element-binding protein CREB binding protein (CREBBP) and E1A binding protein p300 (EP300), n = 3; mRNA expression levels of Crebbp / Ep300 , n = 8. (N) Comparison of m 6 A-MeRIP-qPCR for Crebbp / Ep300 , n = 5. Data are presented as mean ± SEM; ∗ P < 0.05, ∗∗ P < 0.01 vs. respective Control.

Article Snippet: Following SDS–PAGE separation, proteins were transferred to PVDF membranes and probed with specific primary antibodies: H3 (1:2000, ABclonal, A2348), H3K14ac (1:1000, ABclonal, A7254), H3K9ac (1:1000, ABclonal, A7255), H3K27ac (1:1000, ABclonal, A7253), FTO (1:2000, Proteintech, Rosemont, IL, USA, 27226-1-AP), EP300 (1:500, Abcam, ab14984), and CREBBP (1:1000, Santa Cruz, Dallas, TX, USA, SC-365387).

Techniques: Gene Expression, Modification, Dot Blot, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Methylation, Western Blot, Chromatin Immunoprecipitation, ChIP-qPCR, Binding Assay, Comparison, Control