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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting <t>of</t> <t>TLR4,</t> TRAF6, <t>JNK,</t> p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.
Phospho Jnk Rabbit Pab, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting <t>of</t> <t>TLR4,</t> TRAF6, <t>JNK,</t> p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.
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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting <t>of</t> <t>TLR4,</t> TRAF6, <t>JNK,</t> p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.
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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting <t>of</t> <t>TLR4,</t> TRAF6, <t>JNK,</t> p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.
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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting <t>of</t> <t>TLR4,</t> TRAF6, <t>JNK,</t> p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.
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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting <t>of</t> <t>TLR4,</t> TRAF6, <t>JNK,</t> p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.
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Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.

Journal: Microorganisms

Article Title: Synergistic Inhibition of Acinetobacter baumannii Biofilm Formation and Reduction of Lung Inflammation In Vivo by Combination of α-Pinene and Meropenem

doi: 10.3390/microorganisms14050968

Figure Lengend Snippet: Effect of α-pinene combined with MEM on MAPK pathway in lung tissues of mice. ( A ) Immunoblotting of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 proteins in lung tissue lysates. ( B – F ) Western blot analysis of TLR4, TRAF6, JNK, p38 MAPK, and ERK2 in lung tissues. Graphs show quantitative results of gray values after ImageJ analysis. ( G , H ) Levels of cytokine IL-6 and TNF-α in lung tissues. Mean ± S.D, n = 3. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns (black): p > 0.05 compared with the model group. #: p < 0.05; ##: p < 0.01; ns (blue): p > 0.05 compared with the normal group.

Article Snippet: The primary antibodies used included anti-Toll-like receptor 4 Rabbit PAb (TLR4, A5258), p38 MAPK Rabbit pAb (A0227), ERK2 Rabbit pAb (A0229), Phospho-JNK Rabbit pAb (AP0276), TRAF6 Rabbit pAb (A16991), β-actin Rabbit pAb (AC038), Vinculin Rabbit pAb (A14193), and HRP Goat Anti-Mouse IgG1 (AS066), all purchased from ABclonal (Nanjing, China) Technology Co., Ltd.

Techniques: Western Blot