Journal: Cell Communication and Signaling : CCS

Article Title: Atorvastatin promotes lipid catabolism in colorectal cancer via FDFT1‒mediated inhibition of the PI3K/AKT pathway

doi: 10.1186/s12964-026-02802-6

Figure Lengend Snippet: ATST induces ER stress and promotes apoptosis in CRC cells A Expression of ER stress‒related proteins in HCT116 cells after ATST treatment B Changes in intracellular Ca 2+ levels in HCT116 cells C Alterations in XBP‒1s mRNA expression in HCT116 cells D Representative IF images of XBP‒1s in HCT116 cells after ATST treatment. Scale bar: 50 μm E Apoptosis rate in HCT116 cells after ATST treatment F Changes in JC‒1 ratio in HCT116 cells following ATST treatment G Expression of apoptosis‒related proteins in HCT116 cells after ATST treatment H Representative IF images of CHOP in HCT116 cells after ATST treatment. Scale bar: 50 μm I Expression of SQSTM1 and LC3 A/B proteins in HCT116 cells after ATST treatment J Change in ROS levels in HCT116 cells K Cell cycle distribution in HCT116 cells after ATST treatment

Article Snippet: Cells were seeded in 6‒well plates at a density of 5 × 105 cells per well and treated with ATST at specified concentrations for 48 h. Cells were then collected and incubated with JC‒1 working solution (Yesen, 717164ES03, Shanghai) at 37 °C for 20 min. After washing twice with serum‒containing medium, samples were analysed by flow cytometry.

Techniques: Expressing