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Exogenous treatment with <t>Jasmonic</t> <t>acid</t> effectively stimulates the production of PTX. A . The level of JA in the TmLOX13 -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).
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jasmonic acid ja  (Tokyo Chemical Industry)
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Fig. 2. Changes in accumulated amounts of phytoalexins and plant hormones in Nipponbare (NB) and WRC68 (68) cultivars induced by the attack of brown planthoppers (B: BPH) and green rice leafhoppers (G: GRL). The control leaves were incubated without insects (C). After incubations of rice seedlings with BPH and GRL for 13 and 18 day, the aerial parts of the rice plants were separated into uppermost, middle, and lowermost from the leaf tip to bottom. The leaf segments were extracted with 80% methanol and subjected to LC-MS/MS analyses in MRM for phytoalexins and plant hormones. The detected amounts were normalized and expressed as a cell plot. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: <t>jasmonic</t> acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, and <t>ABA:</t> <t>abscisic</t> acid.
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jasmonic acid  (Shanghai Yuanye Biochemicals)
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Fig. 2. Changes in accumulated amounts of phytoalexins and plant hormones in Nipponbare (NB) and WRC68 (68) cultivars induced by the attack of brown planthoppers (B: BPH) and green rice leafhoppers (G: GRL). The control leaves were incubated without insects (C). After incubations of rice seedlings with BPH and GRL for 13 and 18 day, the aerial parts of the rice plants were separated into uppermost, middle, and lowermost from the leaf tip to bottom. The leaf segments were extracted with 80% methanol and subjected to LC-MS/MS analyses in MRM for phytoalexins and plant hormones. The detected amounts were normalized and expressed as a cell plot. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: <t>jasmonic</t> acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, and <t>ABA:</t> <t>abscisic</t> acid.
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Fig. 2. Changes in accumulated amounts of phytoalexins and plant hormones in Nipponbare (NB) and WRC68 (68) cultivars induced by the attack of brown planthoppers (B: BPH) and green rice leafhoppers (G: GRL). The control leaves were incubated without insects (C). After incubations of rice seedlings with BPH and GRL for 13 and 18 day, the aerial parts of the rice plants were separated into uppermost, middle, and lowermost from the leaf tip to bottom. The leaf segments were extracted with 80% methanol and subjected to LC-MS/MS analyses in MRM for phytoalexins and plant hormones. The detected amounts were normalized and expressed as a cell plot. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: <t>jasmonic</t> acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, and <t>ABA:</t> <t>abscisic</t> acid.
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jasmonic acid-isoleucine (ja-ile)  (Medicago)
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Fig. 2. Changes in accumulated amounts of phytoalexins and plant hormones in Nipponbare (NB) and WRC68 (68) cultivars induced by the attack of brown planthoppers (B: BPH) and green rice leafhoppers (G: GRL). The control leaves were incubated without insects (C). After incubations of rice seedlings with BPH and GRL for 13 and 18 day, the aerial parts of the rice plants were separated into uppermost, middle, and lowermost from the leaf tip to bottom. The leaf segments were extracted with 80% methanol and subjected to LC-MS/MS analyses in MRM for phytoalexins and plant hormones. The detected amounts were normalized and expressed as a cell plot. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: <t>jasmonic</t> acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, and <t>ABA:</t> <t>abscisic</t> acid.
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Exogenous treatment with Jasmonic acid effectively stimulates the production of PTX. A . The level of JA in the TmLOX13 -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).

Journal: Scientific Reports

Article Title: A new 13-lipoxygenase from Taxus media callus is functionally linked to paclitaxel biosynthesis

doi: 10.1038/s41598-025-26256-x

Figure Lengend Snippet: Exogenous treatment with Jasmonic acid effectively stimulates the production of PTX. A . The level of JA in the TmLOX13 -silenced cells compared to control cells. B. The amount of secreted PTX in the medium of TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated. C . Relative expression of key genes involved in PTX biosynthesis ( TXS , DBAT and DBTNBT ) in both TmLOX13 -silenced cells treated with 100 µM of JA compared to non-treated on days 8, 12, 16, 20 and 24 after inoculation (DAI). The color scale (black-red-white-green-black) indicates the relative changes of transcript abundance from − 20 to + 20-fold. All measurements were performed in triplicate. Data are mean values ± SD ( n = 3). Statistical significance was evaluated by ANOVA analysis. Asterisks indicate significant differences between JA-treated samples and their respective control samples ( * P < 0.05).

Article Snippet: Jasmonic acid (purity > 98%) was procured Larodan, Inc., Sweden.

Techniques: Control, Expressing

Fig. 2. Changes in accumulated amounts of phytoalexins and plant hormones in Nipponbare (NB) and WRC68 (68) cultivars induced by the attack of brown planthoppers (B: BPH) and green rice leafhoppers (G: GRL). The control leaves were incubated without insects (C). After incubations of rice seedlings with BPH and GRL for 13 and 18 day, the aerial parts of the rice plants were separated into uppermost, middle, and lowermost from the leaf tip to bottom. The leaf segments were extracted with 80% methanol and subjected to LC-MS/MS analyses in MRM for phytoalexins and plant hormones. The detected amounts were normalized and expressed as a cell plot. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: jasmonic acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, and ABA: abscisic acid.

Journal: Journal of Pesticide Science

Article Title: Accumulation of phytoalexins and alteration in plant hormone profiles in rice leaves following sap sucking by the brown planthopper and green rice leafhopper

doi: 10.1584/jpestics.D25-047

Figure Lengend Snippet: Fig. 2. Changes in accumulated amounts of phytoalexins and plant hormones in Nipponbare (NB) and WRC68 (68) cultivars induced by the attack of brown planthoppers (B: BPH) and green rice leafhoppers (G: GRL). The control leaves were incubated without insects (C). After incubations of rice seedlings with BPH and GRL for 13 and 18 day, the aerial parts of the rice plants were separated into uppermost, middle, and lowermost from the leaf tip to bottom. The leaf segments were extracted with 80% methanol and subjected to LC-MS/MS analyses in MRM for phytoalexins and plant hormones. The detected amounts were normalized and expressed as a cell plot. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: jasmonic acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, and ABA: abscisic acid.

Article Snippet: Jasmonic acid (JA), abscisic acid (ABA), and isoorientin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Techniques: Control, Incubation, Liquid Chromatography with Mass Spectroscopy

Fig. 3. Multivariate analysis of accumulated amounts of phytoalexins and plant hormone levels in NB and WRC68 cultivars, following attack by BPH and GRL. The data were normalized and subjected to PCA analysis: (A) score plot, (B) loading plot and (C) correlation analysis. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: jasmonic acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, ABA: abscisic acid.

Journal: Journal of Pesticide Science

Article Title: Accumulation of phytoalexins and alteration in plant hormone profiles in rice leaves following sap sucking by the brown planthopper and green rice leafhopper

doi: 10.1584/jpestics.D25-047

Figure Lengend Snippet: Fig. 3. Multivariate analysis of accumulated amounts of phytoalexins and plant hormone levels in NB and WRC68 cultivars, following attack by BPH and GRL. The data were normalized and subjected to PCA analysis: (A) score plot, (B) loading plot and (C) correlation analysis. MA: momilactone A, PA: phytocassane A, OS: oryzalexin S, OL: oryzalactone, SA: salicylic acid, SAG: salicylic acid glucoside, JA: jasmonic acid, JAIle: jasmonic acid isoleucine conjugate, IAA: indole-3-acetic acid, iP: isopentenyladenine, tZ: trans -zeatin, ABA: abscisic acid.

Article Snippet: Jasmonic acid (JA), abscisic acid (ABA), and isoorientin were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Techniques: