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Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h <t>IVM</t> in ET-1, <t>DMSO,</t> <t>BQ123.</t> (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).
Ivm Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitro maturation ivm medium  (Thermo Fisher)
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Thermo Fisher vitro maturation ivm medium
Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h <t>IVM</t> in ET-1, <t>DMSO,</t> <t>BQ123.</t> (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).
Vitro Maturation Ivm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ivm+medium/Forma+Water+Jacketed+CO2+Incubator/pm41062456-355-18-22
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sage ivm medium  (CooperSurgical)
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CooperSurgical sage ivm medium
Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h <t>IVM</t> in ET-1, <t>DMSO,</t> <t>BQ123.</t> (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).
Sage Ivm Medium, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ivm+medium/sage+ivm+medium/pm40447125-89-125-141
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ivm medium  (Kitazato Corporation)
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Kitazato Corporation ivm medium
Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h <t>IVM</t> in ET-1, <t>DMSO,</t> <t>BQ123.</t> (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).
Ivm Medium, supplied by Kitazato Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ivm medium tcm199  (Thermo Fisher)
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Thermo Fisher ivm medium tcm199
Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h <t>IVM</t> in ET-1, <t>DMSO,</t> <t>BQ123.</t> (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).
Ivm Medium Tcm199, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ivm+medium/tcm+199/pm40281981-92-10-11
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Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h IVM in ET-1, DMSO, BQ123. (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).

Journal: Fundamental Research

Article Title: Single-cell multi-omics analysis decodes molecular characteristics of sheep oocyte fate in vivo maturation

doi: 10.1016/j.fmre.2025.10.009

Figure Lengend Snippet: Modulating sheep oocyte developmental fate through ET-1 supplement. (a) The rate of polar body extrusion after 18 h IVM in ET-1, DMSO, BQ123. (b) The fluorescence intensity of ROS signals was compared in ET-1, DMSO, and BQ123. (c) Representative images of ROS levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 50 µm. Representative images of GSH levels of mature oocytes in the ET-1, DMSO, BQ123. Scale bar, 100 µm. (d) The fluorescence intensity of GSH signals was compared in ET-1, DMSO, and BQ123. (e) Representative images of Mitochondrial membrane potential (ΔΨm), detected by JC-1 staining in ET-1, DMSO, BQ123. (f) The ratio of red to green fluorescence intensity indicates the mitochondrial membrane potential level in the ET-1, DMSO, BQ123. (g) Representative images of the mitochondrial distribution of mature oocytes in the ET-1, DMSO, BQ123, detected by Mito-Tracker Red. Scale bar = 50 µm. (h) The rate of abnormal mitochondrial distribution in ET-1, DMSO, BQ123. (i) Representative images of the CGs distribution of an equatorial section of mature oocytes in the ET-1, DMSO, BQ123, detected by FITC-PNA. Scale bar = 50 µm. (j) The rate of normal CGs distribution in ET-1, DMSO, BQ123. (k) Representative images of the spindle morphology and chromosome alignment of mature oocytes in the ET-1, DMSO, BQ123, detected by immunofluorescent staining. Scale bar = 5 µm. (l) The rate of normal Spindle/chromosome distribution in ET-1, DMSO, BQ123. (m) Representative images of the blastocyst after 7.5 dpi IVF of ET-1, DMSO, BQ123. (n) The rate of blastocyst formation at 7.5 dpi was compared in ET-1, DMSO, and BQ123. (o) Representative images of immunofluorescent staining of CDX2 and OCT4 in blastocysts in ET-1, DMSO, BQ123, with DAPI for DNA. Scale bar = 50 µm. (p) The ratio of Blastocysts’ ICM/TE was compared in ET-1, DMSO, and BQ123. Letters that are the same indicate no significant difference ( P > 0.05), while different letters indicate significant differences ( P ≤ 0.05).

Article Snippet: To screen for the optimal working concentration, ET-1 (Sigma, 05-23-3800) and BQ123 (MCE, HY-12378) were supplemented in the IVM medium at concentrations of 1 nM, 10 nM, and 100 nM, respectively.

Techniques: Fluorescence, Membrane, Staining