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Illustration of liver tissue homogenate before and after enzymatic digestion (A) Example of tubes containing minced tissue in a mixture of RPMI-1640 medium with Collagenase IV and DNase I reagents, (B) Tube showing digested tissue in the enzymatic solution.

Journal: STAR Protocols

Article Title: Protocol for optimized mononuclear cell isolation from liver and tumor tissue using mechanical or enzymatic digestion

doi: 10.1016/j.xpro.2025.104289

Figure Lengend Snippet: Illustration of liver tissue homogenate before and after enzymatic digestion (A) Example of tubes containing minced tissue in a mixture of RPMI-1640 medium with Collagenase IV and DNase I reagents, (B) Tube showing digested tissue in the enzymatic solution.

Article Snippet: Thawing and transferring cell culture reagents. a. Thaw DNase I, Collagenase IV, to reach 20°C–25°C (Only Protocol 2). b. Thaw Gibco Recovery Cell Culture Freezing Medium.

Techniques: