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ist-mes-1  (Interlab Inc)
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Interlab Inc ist-mes-1
(A) CG98 and <t>MPP89</t> cells were stably transduced with a CD157-specific shRNA (sh-CD157) or a scrambled shRNA (sh-control). (B) MSTO and REN cells were stably transfected with the pcDNA3.1 expression vector containing full-length CD157 cDNA (pcDNA-CD157), or with the empty vector (pcDNA-control). CD157 mRNA and protein expression were analysed by RT-PCR, western blot and flow cytometry. GAPDH, β-actin and an isotype matched mAb (gray shaded peaks) were used as controls, respectively. (C) Light microscopy images showing the morphological features of cells with or without CD157 stained with crystal violet and reproduced in black and white (magnification ×10). (D) Western blot for fibronectin. (E) MTT viability assay of MPM cells. Results represent the mean ± SEM of three independent experiments performed in quadruplicate. (F) Colony formation assay of MPM cells. Colonies were stained with crystal violet and reproduced in black and white. (G) Clonogenic assay in soft agar. Columns represent the average number of colonies/10 fields from three independent experiments ± SEM. **** p < 0.0001, *** p < 0.001,** p < 0.01,* p < 0.05, ns = not significant, two-tailed unpaired t test.
Ist Mes 1, supplied by Interlab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ist-mes-1 - by Bioz Stars, 2026-11
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(A) CG98 and MPP89 cells were stably transduced with a CD157-specific shRNA (sh-CD157) or a scrambled shRNA (sh-control). (B) MSTO and REN cells were stably transfected with the pcDNA3.1 expression vector containing full-length CD157 cDNA (pcDNA-CD157), or with the empty vector (pcDNA-control). CD157 mRNA and protein expression were analysed by RT-PCR, western blot and flow cytometry. GAPDH, β-actin and an isotype matched mAb (gray shaded peaks) were used as controls, respectively. (C) Light microscopy images showing the morphological features of cells with or without CD157 stained with crystal violet and reproduced in black and white (magnification ×10). (D) Western blot for fibronectin. (E) MTT viability assay of MPM cells. Results represent the mean ± SEM of three independent experiments performed in quadruplicate. (F) Colony formation assay of MPM cells. Colonies were stained with crystal violet and reproduced in black and white. (G) Clonogenic assay in soft agar. Columns represent the average number of colonies/10 fields from three independent experiments ± SEM. **** p < 0.0001, *** p < 0.001,** p < 0.01,* p < 0.05, ns = not significant, two-tailed unpaired t test.

Journal: Oncotarget

Article Title: CD157 enhances malignant pleural mesothelioma aggressiveness and predicts poor clinical outcome

doi:

Figure Lengend Snippet: (A) CG98 and MPP89 cells were stably transduced with a CD157-specific shRNA (sh-CD157) or a scrambled shRNA (sh-control). (B) MSTO and REN cells were stably transfected with the pcDNA3.1 expression vector containing full-length CD157 cDNA (pcDNA-CD157), or with the empty vector (pcDNA-control). CD157 mRNA and protein expression were analysed by RT-PCR, western blot and flow cytometry. GAPDH, β-actin and an isotype matched mAb (gray shaded peaks) were used as controls, respectively. (C) Light microscopy images showing the morphological features of cells with or without CD157 stained with crystal violet and reproduced in black and white (magnification ×10). (D) Western blot for fibronectin. (E) MTT viability assay of MPM cells. Results represent the mean ± SEM of three independent experiments performed in quadruplicate. (F) Colony formation assay of MPM cells. Colonies were stained with crystal violet and reproduced in black and white. (G) Clonogenic assay in soft agar. Columns represent the average number of colonies/10 fields from three independent experiments ± SEM. **** p < 0.0001, *** p < 0.001,** p < 0.01,* p < 0.05, ns = not significant, two-tailed unpaired t test.

Article Snippet: The human MPM cell lines MSTO-211H (designated MSTO) [ ], MPP89, IST-MES-1 and IST-MES-2 [ ] were purchased from Interlab Cell Line Collection (Genova, Italy), REN [ ] was kindly provided by L. Moro (University of Novara, Italy) who received the cells from S. Albelda (University of Pennsylvania, Philadelphia, USA); CE96, OC99, CG98 and MMP cell lines were obtained from the mesothelioma bio bank, Pathology Unit, City Hospital of Alessandria (Italy) [ ].

Techniques: Stable Transfection, Transduction, shRNA, Transfection, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry, Light Microscopy, Staining, MTT Viability Assay, Colony Assay, Clonogenic Assay, Two Tailed Test