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A. IST-MES-1, <t>IST-MES-2,</t> MSTO-211H and MeT-5A cells were incubated for 5 days in presence of exogenous Peroxynitrite (left panel) or H 2 O 2 (right panel) at different concentrations. Cell viability was measured using CellTiter assay (luminescence). B. Digital representation of data obtained for PRDX3 and PRDX5 detection (capillary western blot) in cell line panel (left panel). Bar plots showing data from one individual run (2 replicates per condition, SD value, right panel).
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(A) CG98 and <t>MPP89</t> cells were stably transduced with a CD157-specific shRNA (sh-CD157) or a scrambled shRNA (sh-control). (B) MSTO and REN cells were stably transfected with the pcDNA3.1 expression vector containing full-length CD157 cDNA (pcDNA-CD157), or with the empty vector (pcDNA-control). CD157 mRNA and protein expression were analysed by RT-PCR, western blot and flow cytometry. GAPDH, β-actin and an isotype matched mAb (gray shaded peaks) were used as controls, respectively. (C) Light microscopy images showing the morphological features of cells with or without CD157 stained with crystal violet and reproduced in black and white (magnification ×10). (D) Western blot for fibronectin. (E) MTT viability assay of MPM cells. Results represent the mean ± SEM of three independent experiments performed in quadruplicate. (F) Colony formation assay of MPM cells. Colonies were stained with crystal violet and reproduced in black and white. (G) Clonogenic assay in soft agar. Columns represent the average number of colonies/10 fields from three independent experiments ± SEM. **** p < 0.0001, *** p < 0.001,** p < 0.01,* p < 0.05, ns = not significant, two-tailed unpaired t test.
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A. IST-MES-1, IST-MES-2, MSTO-211H and MeT-5A cells were incubated for 5 days in presence of exogenous Peroxynitrite (left panel) or H 2 O 2 (right panel) at different concentrations. Cell viability was measured using CellTiter assay (luminescence). B. Digital representation of data obtained for PRDX3 and PRDX5 detection (capillary western blot) in cell line panel (left panel). Bar plots showing data from one individual run (2 replicates per condition, SD value, right panel).

Journal: bioRxiv

Article Title: Identification of non-covalent inhibitors for the atypical peroxiredoxin PRDX5 as a therapeutic strategy in malignant pleural mesothelioma

doi: 10.64898/2026.05.13.724787

Figure Lengend Snippet: A. IST-MES-1, IST-MES-2, MSTO-211H and MeT-5A cells were incubated for 5 days in presence of exogenous Peroxynitrite (left panel) or H 2 O 2 (right panel) at different concentrations. Cell viability was measured using CellTiter assay (luminescence). B. Digital representation of data obtained for PRDX3 and PRDX5 detection (capillary western blot) in cell line panel (left panel). Bar plots showing data from one individual run (2 replicates per condition, SD value, right panel).

Article Snippet: The human malignant pleural mesothelioma (MPM) cell lines IST-MES-1, and IST-MES-2 were obtained from Creative Bioarray.

Techniques: Incubation, Western Blot

(A) CG98 and MPP89 cells were stably transduced with a CD157-specific shRNA (sh-CD157) or a scrambled shRNA (sh-control). (B) MSTO and REN cells were stably transfected with the pcDNA3.1 expression vector containing full-length CD157 cDNA (pcDNA-CD157), or with the empty vector (pcDNA-control). CD157 mRNA and protein expression were analysed by RT-PCR, western blot and flow cytometry. GAPDH, β-actin and an isotype matched mAb (gray shaded peaks) were used as controls, respectively. (C) Light microscopy images showing the morphological features of cells with or without CD157 stained with crystal violet and reproduced in black and white (magnification ×10). (D) Western blot for fibronectin. (E) MTT viability assay of MPM cells. Results represent the mean ± SEM of three independent experiments performed in quadruplicate. (F) Colony formation assay of MPM cells. Colonies were stained with crystal violet and reproduced in black and white. (G) Clonogenic assay in soft agar. Columns represent the average number of colonies/10 fields from three independent experiments ± SEM. **** p < 0.0001, *** p < 0.001,** p < 0.01,* p < 0.05, ns = not significant, two-tailed unpaired t test.

Journal: Oncotarget

Article Title: CD157 enhances malignant pleural mesothelioma aggressiveness and predicts poor clinical outcome

doi:

Figure Lengend Snippet: (A) CG98 and MPP89 cells were stably transduced with a CD157-specific shRNA (sh-CD157) or a scrambled shRNA (sh-control). (B) MSTO and REN cells were stably transfected with the pcDNA3.1 expression vector containing full-length CD157 cDNA (pcDNA-CD157), or with the empty vector (pcDNA-control). CD157 mRNA and protein expression were analysed by RT-PCR, western blot and flow cytometry. GAPDH, β-actin and an isotype matched mAb (gray shaded peaks) were used as controls, respectively. (C) Light microscopy images showing the morphological features of cells with or without CD157 stained with crystal violet and reproduced in black and white (magnification ×10). (D) Western blot for fibronectin. (E) MTT viability assay of MPM cells. Results represent the mean ± SEM of three independent experiments performed in quadruplicate. (F) Colony formation assay of MPM cells. Colonies were stained with crystal violet and reproduced in black and white. (G) Clonogenic assay in soft agar. Columns represent the average number of colonies/10 fields from three independent experiments ± SEM. **** p < 0.0001, *** p < 0.001,** p < 0.01,* p < 0.05, ns = not significant, two-tailed unpaired t test.

Article Snippet: The human MPM cell lines MSTO-211H (designated MSTO) [ ], MPP89, IST-MES-1 and IST-MES-2 [ ] were purchased from Interlab Cell Line Collection (Genova, Italy), REN [ ] was kindly provided by L. Moro (University of Novara, Italy) who received the cells from S. Albelda (University of Pennsylvania, Philadelphia, USA); CE96, OC99, CG98 and MMP cell lines were obtained from the mesothelioma bio bank, Pathology Unit, City Hospital of Alessandria (Italy) [ ].

Techniques: Stable Transfection, Transduction, shRNA, Transfection, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry, Light Microscopy, Staining, MTT Viability Assay, Colony Assay, Clonogenic Assay, Two Tailed Test