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Journal: Cell reports
Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
doi: 10.1016/j.celrep.2024.115126
Figure Lengend Snippet: Figure 3. MBP exposure activated the IRE1a/XBP1s pathway in NSCs (A) Volcano plots display alterations in gene expression of NSCs treated with 1 mM MBP in both proliferation and differentiation media. Downregulated genes are represented by blue dots (p < 0.05 and log2 fold change [log2FC] < –0.58). Upregulated genes are represented by red dots (p < 0.05 and log2FC > 0.58). (B) Top upregulated genes (p < 0.05) are shown in the bubble plot. (C) The heatmap shows the validation of top deregulated genes upon MBP treatment under differentiation conditions using qPCR. (D) The gene sets that exhibited significant enrichment (p < 0.05) were identified and sorted based on their normalized enrichment score.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GRP78 antibody Cell Signaling Technology Cat#3711; RRID: AB_2063354 Anti-PERK antibody Cell Signaling Technology Cat#3192; RRID: AB_2095847 Anti- XBP1s antibody Cell Signaling Technology Cat#12782s; RRID: AB_2687943 Anti- ATF6 antibody Cell Signaling Technology Cat#37149; RRID: AB_725571 Anti- ATF3 antibody Abcam Cat#ab207434; RRID: AB_2734728 Anti- synaptophysin antibody Abcam Cat#ab52636; RRID: 882786 Anti- Homer1 antibody Abcam Cat#ab184955; RRID: 2744679
Techniques: Gene Expression, Biomarker Discovery