Journal: International Journal of Molecular Sciences

Article Title: Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

doi: 10.3390/ijms25105309

Figure Lengend Snippet: ( a ) Chemical structure and tumor-targeting efficiency of ICG. The tumor sites are indicated by arrowheads. Scale bar = 1 cm. ( b ) Chemical structure and ( c ) 3D modeling of IR-783. Red, negative charge; blue, positive charge; gray, hydrophobicity. ( d ) Absorption and fluorescence emission spectra of IR-783 measured in PBS at pH 7.4. ( e ) Physicochemical and optical properties of IR-783. In silico calculations of the distribution coefficient (log D at pH 7.4) and surface charge density distribution were performed using Marvin and JChem calculator plugins (ChemAxon).

Article Snippet: IR-783 and ICG were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.

Techniques: Fluorescence, In Silico

Journal: International Journal of Molecular Sciences

Article Title: Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

doi: 10.3390/ijms25105309

Figure Lengend Snippet: ( a ) Cell viability assay of IR-783 using HT-29 cancer cells. Percentage cytotoxicity is determined after 4 h of treatment with various concentrations of IR-783. ( b ) Live-cell binding of IR-783 in HT-29 cancer cells. Phase contrast and NIR fluorescence images are obtained after 4 h of incubation with 2 μM of IR-783. Images are representative of n = 3 independent experiments. All fluorescence images had identical exposure times and normalization. Scale bars = 100 μm.

Article Snippet: IR-783 and ICG were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.

Techniques: Viability Assay, Binding Assay, Fluorescence, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

doi: 10.3390/ijms25105309

Figure Lengend Snippet: ( a ) In vitro thermal images of PBS and IR-783 (300 μM) solutions irradiated with an 808 nm laser at 1.0 W/cm 2 power density for 1 min. The infrared thermal imager was used to monitor the maximum temperature in real time. ( b ) Photostability of the IR-783 solution under laser irradiation. The absorbance changes were measured before and after 1 min of laser irradiation. ( c ) Temperature changes in PBS and IR-783 (100, 200, and 300 μM) solutions were observed for 120 s of laser irradiation (808 nm, 1.0 W/cm 2 ). ( d ) Heating and cooling curve of IR-783 (300 μM) under laser irradiation (808 nm, 1.0 W/cm 2 ). ( e ) Temperature changes of IR-783 (300 μM) during three on/off cycles of laser irradiation (808 nm, 1.0 W/cm 2 ).

Article Snippet: IR-783 and ICG were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.

Techniques: In Vitro, Irradiation

Journal: International Journal of Molecular Sciences

Article Title: Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

doi: 10.3390/ijms25105309

Figure Lengend Snippet: Fluorescence images of HT-29 cells before and after PTT treatment. HT-29 cells were incubated with the 5 µM concentration of IR-783 for 4 h and treated with the 808 nm laser at 1.0 W/cm 2 for 1 min. HT-29 cells were then costained with calcein-AM (green for live cells) and propidium iodide (PI; red for dead cells). Images are representative of n = 3 independent experiments. All fluorescence images had identical exposure times and normalization. Scale bars = 100 μm.

Article Snippet: IR-783 and ICG were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.

Techniques: Fluorescence, Incubation, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

doi: 10.3390/ijms25105309

Figure Lengend Snippet: In vivo HT-29 tumor-targeting efficiency of IR-783. ( a ) NIR fluorescence imaging for 48 h after injection of IR-783. The tumor sites are indicated by arrowheads. Scale bars = 1 cm. ( b ) Time-dependent fluorescence intensity and ( c ) tumor-to-background ratio at the tumor site targeted by IR-783. The inset shows the resected tumors 4 h and 24 h after injection of IR-783. Abbreviations: Mu, muscle; Tu, tumor; PI, post-injection. ( d ) Thermal images and ( e ) temperature changes in tumor-bearing mice at the tumor area 24 h after injection of PBS or IR-783, followed by 808 nm laser irradiation (1.0 W/cm 2 ) for 5 min. Images are representative of 3 mice per treatment group. All NIR fluorescence images had identical exposure times and normalization. Data are expressed as mean ± S.D. (n = 3). ** p < 0.01.

Article Snippet: IR-783 and ICG were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Irradiation

Journal: International Journal of Molecular Sciences

Article Title: Structure-Inherent Tumor-Targeted IR-783 for Near-Infrared Fluorescence-Guided Photothermal Therapy

doi: 10.3390/ijms25105309

Figure Lengend Snippet: In vivo NIR phototherapeutic efficacy of IR-783. ( a ) Representative photos of changes in tumor size in HT-29 tumor-bearing mice for 9 days after different treatments. The laser groups were treated with 24 h post-injections of PBS or IR-783, followed by 808 nm laser irradiation (1.0 W/cm 2 ) for 5 min. Scale bars = 1 cm. ( b ) Tumor growth rates and ( c ) body weights of each treatment group were monitored for 9 days. Data are expressed as mean ± S.D. (n = 3). *** p < 0.001. n.s., not significant. ( d ) Histological observation of tumors stained with H&E in each treatment group. Scale bars = 50 μm.

Article Snippet: IR-783 and ICG were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received without further purification.

Techniques: In Vivo, Irradiation, Staining