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Cytokine immune response in serum, the in vitro system, and tissue samples. The Th-1 (IFN-γ and IL-12) and Th-2 (IL-4 and IL-10) cytokines were quantified in the serum of immunized animals using ELISA at 2 weeks post-booster: IFN-γ (A) , IL-10 (B) , IL-12 (C) , IL-4 (D) , and IFN-γ/IL-10 ratio (E) . Twelve weeks post-infection IFN-γ (F) , IL-10 (G) , IL-12 (H) , IL-4 (I) , and IFN-γ/IL-10 ratio (J) . All experiments were performed in triplicate, having three biological replicates in each experiment and data are presented here as the mean and standard error. Different vaccinated groups were compared to determine the statistical significance of the data using ANOVA with the Tukey test analysis with p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) level of significance.

Journal: Frontiers in Immunology

Article Title: Mycobacterium tuberculosis antigen-containing exosomes reinforce BCG vaccine efficacy by augmenting long-term protection and memory response against experimental tuberculosis in BALB-C mice

doi: 10.3389/fimmu.2026.1742207

Figure Lengend Snippet: Cytokine immune response in serum, the in vitro system, and tissue samples. The Th-1 (IFN-γ and IL-12) and Th-2 (IL-4 and IL-10) cytokines were quantified in the serum of immunized animals using ELISA at 2 weeks post-booster: IFN-γ (A) , IL-10 (B) , IL-12 (C) , IL-4 (D) , and IFN-γ/IL-10 ratio (E) . Twelve weeks post-infection IFN-γ (F) , IL-10 (G) , IL-12 (H) , IL-4 (I) , and IFN-γ/IL-10 ratio (J) . All experiments were performed in triplicate, having three biological replicates in each experiment and data are presented here as the mean and standard error. Different vaccinated groups were compared to determine the statistical significance of the data using ANOVA with the Tukey test analysis with p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) level of significance.

Article Snippet: Serum samples collected pre-immunization, post-boost, and 12 weeks post-challenge were analyzed for IgG1 (KLM1629), IgG2a (KLM1488), IFN-γ (KB2011), IL-12 (KLM0020), IL-4 (KLM0051), and IL-10 (KB2072) using GENLISA sandwich ELISA kits (Krishgen BioSystems, USA, catalog numbers as specified).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Infection