Journal: Physiologia Plantarum

Article Title: Microbial Inoculation Strategies for Optimal Cherry Tomato Production

doi: 10.1111/ppl.70655

Figure Lengend Snippet: Morphometric characteristics at 15 and 30 days after planting (DAS). Radicle length at 15 DAS (a) and roots at 30 DAS (b) of cherry tomato seedlings from treatments non‐inoculation (NI), inoculated with Bacillus subtilis ATCC 23858 (BA) and Burkholderia seminalis TC3.4.2R3 (BU).

Article Snippet: The principal component analysis in Figure shows the main responses from the set of variables, considering the main samples of inoculation types ( Bacillus subtilis ATCC 23858— Bacillus subtilis , Burkholderia seminalis TC3.4.2R3— Burkholderia seminalis , and non‐inoculation—control treatment).

Techniques:

Journal: Physiologia Plantarum

Article Title: Microbial Inoculation Strategies for Optimal Cherry Tomato Production

doi: 10.1111/ppl.70655

Figure Lengend Snippet: Relative growth rate of cherry tomato non‐inoculation and inoculated with Bacillus subtilis ATCC 23858 and Burkholderia seminalis TC3.4.2R3. Significant at the 1% Tukey test level.

Article Snippet: The principal component analysis in Figure shows the main responses from the set of variables, considering the main samples of inoculation types ( Bacillus subtilis ATCC 23858— Bacillus subtilis , Burkholderia seminalis TC3.4.2R3— Burkholderia seminalis , and non‐inoculation—control treatment).

Techniques:

Journal: mSphere

Article Title: Mouse Genetic Background Affects Transfer of an Antibiotic Resistance Plasmid in the Gastrointestinal Tract

doi: 10.1128/mSphere.00847-19

Figure Lengend Snippet: Experimental setup. Mice harbored a gastrointestinal community consisting of the altered Schaedler flora (ASF) or a conventional microbiota (CONV). One week before the inoculation of 10 8 CFU of Escherichia coli strain HS-4 on day 0, fecal samples were collected to confirm the E. coli - and Salmonella -negative status of the mice. On day 7, the mice were inoculated with 10 8 CFU (unless otherwise indicated) of Salmonella strain CVM29188. On day 21, dextran sodium sulfate (DSS) was added to the drinking water at a final concentration of 2% and remained there until day 28 for all mice except IL-10 −/− 129S6/SvEv ASF mice, which did not receive DSS. Fecal samples were collected only to quantify the bacterial strains by agar plating (gray squares) or for quantifying both bacterial strains by agar plating and microbial members of the gastrointestinal tract by qPCR (blue squares).

Article Snippet: Gnotobiotic C3H/HeN ASF mice (∼17 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 8 CFU of CVM29188 (∼19 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 2 to 10 6 CFU of CVM29188 (10 to 14 weeks old, n = 8/group), and IL-10 −/− 129S6/SvEv ASF mice (10 to 12 weeks old, n = 7) were originally obtained from Taconic Biosciences.

Techniques: Concentration Assay

Journal: mSphere

Article Title: Mouse Genetic Background Affects Transfer of an Antibiotic Resistance Plasmid in the Gastrointestinal Tract

doi: 10.1128/mSphere.00847-19

Figure Lengend Snippet: The donor inoculum concentration does not greatly impact the transconjugant yield. Direct plate counts from fecal pellets of 129S6/SvEv mice harboring the altered Schaedler flora inoculated on day 0 with 10 8 CFU of recipient Escherichia coli HS-4 (blue symbols) and on day 7 with 10 2 (A), 10 4 (B), 10 6 (C), or 10 8 (D) CFU of donor Salmonella Kentucky CVM29188 (purple symbols) were determined. (E) Transconjugants from each donor concentration are overlaid (red symbols). Each symbol represents the mean (8 mice/group), and error bars represent standard deviations. Significant differences ( P < 0.05), as indicated by an asterisk, were determined by an ANOVA, followed by Tukey’s test for multiple means comparison. The horizontal dashed line is the limit of quantification (LOQ). Samples that tested negative were assigned a value halfway between 0 and the LOQ, and undiluted samples with greater than 0 but less than 20 colonies were assigned a value of the LOQ.

Article Snippet: Gnotobiotic C3H/HeN ASF mice (∼17 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 8 CFU of CVM29188 (∼19 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 2 to 10 6 CFU of CVM29188 (10 to 14 weeks old, n = 8/group), and IL-10 −/− 129S6/SvEv ASF mice (10 to 12 weeks old, n = 7) were originally obtained from Taconic Biosciences.

Techniques: Concentration Assay, Comparison

Journal: mSphere

Article Title: Mouse Genetic Background Affects Transfer of an Antibiotic Resistance Plasmid in the Gastrointestinal Tract

doi: 10.1128/mSphere.00847-19

Figure Lengend Snippet: Mouse genetic background affects transconjugant yield. Direct plate counts from fecal pellets from 129S6/SvEv ASF mice (A) and C3H/HeN ASF mice (B) and an overlay of the transconjugant yields from 129S6/SvEv ASF mice (red diamonds) and C3H/HeN ASF mice (red triangles) (C) are shown. Mice were inoculated with recipient Escherichia coli HS-4 (blue symbols) on day 0 and donor Salmonella Kentucky CVM29188 (purple symbols) on day 7. Data are representative of those from two individual experiments. Each symbol represents the mean (8 mice/group), and error bars represent standard deviations. The horizontal dashed line is the limit of quantification (LOQ). Samples that tested negative were assigned a value halfway between 0 and the LOQ, and undiluted samples with greater than 0 but less than 20 colonies were assigned a value of the LOQ. Significant differences ( P < 0.05) were determined by a t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: Gnotobiotic C3H/HeN ASF mice (∼17 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 8 CFU of CVM29188 (∼19 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 2 to 10 6 CFU of CVM29188 (10 to 14 weeks old, n = 8/group), and IL-10 −/− 129S6/SvEv ASF mice (10 to 12 weeks old, n = 7) were originally obtained from Taconic Biosciences.

Techniques:

Journal: mSphere

Article Title: Mouse Genetic Background Affects Transfer of an Antibiotic Resistance Plasmid in the Gastrointestinal Tract

doi: 10.1128/mSphere.00847-19

Figure Lengend Snippet: Escherichia coli and Salmonella strains with associated plasmids

Article Snippet: Gnotobiotic C3H/HeN ASF mice (∼17 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 8 CFU of CVM29188 (∼19 weeks old, n = 8), wild-type 129S6/SvEv ASF mice inoculated with 10 2 to 10 6 CFU of CVM29188 (10 to 14 weeks old, n = 8/group), and IL-10 −/− 129S6/SvEv ASF mice (10 to 12 weeks old, n = 7) were originally obtained from Taconic Biosciences.

Techniques: Plasmid Preparation, Mutagenesis

Journal: Cancer Science

Article Title: Investigating the hepatitis B virus life cycle using engineered reporter hepatitis B viruses

doi: 10.1111/cas.13440

Figure Lengend Snippet: Trans‐ rescue experiment of reporter hepatitis B virus ( HBV ) production. Reporter viruses were harvested from HepG2 transfected with the indicated plasmids together with pHBV /D. The viruses were used to infect 2 × 10 5 PXB cells in the presence or absence of 100 copies per cell of HBV obtained from primary human hepatocytes ( PHH ) maintained in urokinase‐type plasminogen activator transgenic/ SCID mice. Virus was prepared from the medium and used to infect 1 × 10 5 HuH7/ NTCP cells. NanoLuc ( NL ) activity was measured at the indicated times. On day 16, an aliquot of the cells was treated with 80 nmol/L entecavir. Solid lines indicate relative NL activity in HuH7/ NTCP infected with culture medium recovered from PXB cells infected with HBV / NL (red), HBV / NLS +pol (green) and HBV / NLS +polS (blue) together with wild‐type HBV . Dotted lines indicate relative NL activity in cells infected with culture medium recovered from PXB cells infected with HBV / NL (red), HBV / NLS +pol (green) and HBV / NLS +polS (blue) without wild‐type HBV . Double lines with red ( HBV / NL ) and with green ( HBV / NLS +pol) show NL activity after entecavir treatment. Experiments were conducted twice and the mean value is plotted in this figure

Article Snippet: Primary human hepatocytes (PHH), PXB cells, isolated from urokinase‐type plasminogen activator transgenic/SCID mice inoculated with PHH (PhoenixBio, Hiroshima, Japan), were cultured in the medium according to the manufacturer's protocol.

Techniques: Virus, Transfection, Transgenic Assay, Activity Assay, Infection