Journal: Investigative Ophthalmology & Visual Science

Article Title: Cholesterol Accumulation Promotes Photoreceptor Senescence and Retinal Degeneration

doi: 10.1167/iovs.65.10.29

Figure Lengend Snippet: Selective elimination of p16-positive senescent cells suppresses retinal dysfunction and degeneration in rod-specific cholesterol efflux-deficient mice. ( A ) Experimental design using Abca1/g1 -rod/-rod ;INK-ATTAC. ( B ) Electroretinography waveform of Abca1/g1 -rod/-rod and Abca1/g1 -rod/-rod ;INK-ATTAC. Each waveform and graph indicate the mean ± SEM. ( C ) The quantification of ERG (scotopic a-wave and b-wave) amplitudes. ( D ) Images of H&E staining. Scale bar : 50 µm. * P < 0.05; ** P < 0.01, two-way ANOVA followed by Bonferroni correction. Data are represented as mean ± SEM.

Article Snippet: To generate mice deficient in Abca1 and Abcg1 in photoreceptors ( Abca1/g1 -rod/-rod ), Abca1 flox/flox /Abcg1 flox/flox ( Abca1/g1 f/f ) mice purchased from the Jackson Laboratory (021067) were crossed with mice with Rhodopsin-iCre75 transgene mice, which were originally provided by Dr. Ching-Kang Jason Chen., INK-ATTAC transgene mice were provided by Unity Biotechnology 33 and crossed with Abca1/g1 -rod/-rod to generate Abca1/g1 -rod/-rod ;INK-ATTAC.

Techniques: Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Cholesterol Accumulation Promotes Photoreceptor Senescence and Retinal Degeneration

doi: 10.1167/iovs.65.10.29

Figure Lengend Snippet: Selective elimination of p16-positive senescent cells suppresses AMD-associated phenotype and senescence without altering systemic lipid metabolism. Representative electron microscopy images showing RPE cells and Bruch's membrane ( A ), and the quantification of the number of intracellular lipids in RPE cells ( B ) and Bruch's membrane thickness ( C ). Scale bar : 2 µm. Note intracellular lipids in RPE cells ( arrow ) and thickened Bruch's membrane ( arrowhead ) in Abca1/g1 -rod/-rod . ( D ) mRNA expression of senescence markers ( p16 and p21 ) and Tnf-α in the retina and RPE/choroid complex isolated from Abca1/g1 -rod/-rod and Abca1/g1 -rod/-rod ;INK-ATTAC. Fat percentage ( E ), cholesterol ester in plasma and liver (F), and triglyceride and free fatty acids in adipose tissue ( G ) of Abca1/g1 -rod/-rod and Abca1/g1 -rod/-rod ;INK-ATTAC. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001, t -test. Data are represented as mean ± SEM.

Article Snippet: To generate mice deficient in Abca1 and Abcg1 in photoreceptors ( Abca1/g1 -rod/-rod ), Abca1 flox/flox /Abcg1 flox/flox ( Abca1/g1 f/f ) mice purchased from the Jackson Laboratory (021067) were crossed with mice with Rhodopsin-iCre75 transgene mice, which were originally provided by Dr. Ching-Kang Jason Chen., INK-ATTAC transgene mice were provided by Unity Biotechnology 33 and crossed with Abca1/g1 -rod/-rod to generate Abca1/g1 -rod/-rod ;INK-ATTAC.

Techniques: Electron Microscopy, Membrane, Expressing, Isolation

Journal: Cell reports

Article Title: LXR/CD38 activation drives cholesterol-induced macrophage senescence and neurodegeneration via NAD + depletion

doi: 10.1016/j.celrep.2024.114102

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse: INK-ATTAC , Unity Biotechnology , N/A.

Techniques: Virus, Recombinant, Reverse Transcription, Transfection, Cell Culture, RNAscope, Bicinchoninic Acid Protein Assay, Staining, Activity Assay, TUNEL Assay, In Situ, Membrane, Labeling, Sequencing, Isolation, Negative Control, Software