Journal: Scientific Reports

Article Title: A fluorometric assay to determine labile copper(II) ions in serum

doi: 10.1038/s41598-023-39841-9

Figure Lengend Snippet: Overview of human serum samples in this study.

Article Snippet: A set of commercially available individual human serum samples (N = 96, Table ) (in.vent Diagnostica GmbH, Hennigsdorf, Germany) served as a reference cohort for healthy individuals within this study.

Techniques:

Journal: Scientific Reports

Article Title: Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines

doi: 10.1038/s41598-023-29072-3

Figure Lengend Snippet: Seroprevalence of four endemic HCoVs and SARS-CoV-2 among different age groups of children who were collected before the COVID-19 pandemic (2015–2018) in the Philippines. The bar chart represents the percentage of serum samples that were positive for HCoV-229E (blue), HCoV-NL63 (red), HCoV-HKU1 (green), HCoV-OC43 (purple), and SARS-CoV-2 (orange) in each age group. The number of samples per age group is indicated in brackets.

Article Snippet: Serum samples from SARS-CoV-2-infected individuals were purchased from commercial laboratory (RayBiotech, GA, USA) and served as positive controls.

Techniques:

Journal: Scientific Reports

Article Title: Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines

doi: 10.1038/s41598-023-29072-3

Figure Lengend Snippet: Anti-SARS-CoV-2 antibodies, assessed by ELISA, according to seroreactive to four HCoVs and age in children who were collected before the COVID-19 pandemic (2015–2018). Seroreactive of the sera to ( a ) HCoV-229E, ( b ) HCoV-NL63, ( c ) HCoV-HKU1, and ( d ) HCoV-OC43, assessed by ELISA against the respective spike proteins of the different viruses. ( e ) Anti-SARS-CoV-2 antibodies according to age (negative: n = 246; positive: n = 69). The Y axis represents the ratio to cut-off-value of each antigen, or age, compared with Mann–Whitney U tests. Horizontal lines indicate mean values.

Article Snippet: Serum samples from SARS-CoV-2-infected individuals were purchased from commercial laboratory (RayBiotech, GA, USA) and served as positive controls.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines

doi: 10.1038/s41598-023-29072-3

Figure Lengend Snippet: Correlation analysis of the cut-off-value ratio to the four HCoVs and to SARS-CoV-2 in children who were collected before the COVID-19 pandemic (2015–2018). Positive correlations are symbolized by graded shades of red, and negative correlations are by graded shades of blue. The coefficient of determination (R 2 ) for each pair of viruses are indicated.

Article Snippet: Serum samples from SARS-CoV-2-infected individuals were purchased from commercial laboratory (RayBiotech, GA, USA) and served as positive controls.

Techniques:

Journal: Scientific Reports

Article Title: Seroprevalence of four endemic human coronaviruses and, reactivity and neutralization capability against SARS-CoV-2 among children in the Philippines

doi: 10.1038/s41598-023-29072-3

Figure Lengend Snippet: Assessment by PRNT of the neutralizing effect of the SARS-CoV-2 reactive sera. Percentage of inhibition of SARS-CoV-2 infection by reactive sera determined by PRNT in comparison with vehicle control. For each serum, the dot plot shows the correlation between the anti-SARS-CoV-2 reactivity, assessed by ELISA, and the neutralization, assessed by PRNT. A sample from a SARS-CoV-2-infected individual was used as a positive control (PC). SARS-CoV-2 non-reactive samples (negative) are represented with blue circles (n = 5), SARS-CoV-2 reactive samples (positive) are represented with black circles (n = 69). Data are compared by Spearman’s rank correlation coefficient.

Article Snippet: Serum samples from SARS-CoV-2-infected individuals were purchased from commercial laboratory (RayBiotech, GA, USA) and served as positive controls.

Techniques: Inhibition, Infection, Enzyme-linked Immunosorbent Assay, Neutralization, Positive Control