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a Gross morphology of control and Orai1 BKO mice (30 weeks). b Body composition analysis by NMR of control and Orai1 BKO mice (30 weeks). c indirect <t>calorimetry</t> measurements (20 weeks). d Body weight curves under HFD conditions (n=11~13 mice per group). e, f Glucose tolerance test ( e ) and insulin tolerance test ( f ) in control and Orai1 BKO mice fed HFD. g Representative H&E staining images of BAT and liver. h Serum biochemical analysis of lipid profiles. i qRT-PCR analysis of thermogenic genes in BAT. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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a Gross morphology of control and Orai1 BKO mice (30 weeks). b Body composition analysis by NMR of control and Orai1 BKO mice (30 weeks). c indirect calorimetry measurements (20 weeks). d Body weight curves under HFD conditions (n=11~13 mice per group). e, f Glucose tolerance test ( e ) and insulin tolerance test ( f ) in control and Orai1 BKO mice fed HFD. g Representative H&E staining images of BAT and liver. h Serum biochemical analysis of lipid profiles. i qRT-PCR analysis of thermogenic genes in BAT. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: bioRxiv

Article Title: Orai1-mediated Ca 2+ Entry Regulates Lipolysis and Mitochondrial Activation in Brown Adipose Thermogenesis

doi: 10.64898/2026.04.24.718619

Figure Lengend Snippet: a Gross morphology of control and Orai1 BKO mice (30 weeks). b Body composition analysis by NMR of control and Orai1 BKO mice (30 weeks). c indirect calorimetry measurements (20 weeks). d Body weight curves under HFD conditions (n=11~13 mice per group). e, f Glucose tolerance test ( e ) and insulin tolerance test ( f ) in control and Orai1 BKO mice fed HFD. g Representative H&E staining images of BAT and liver. h Serum biochemical analysis of lipid profiles. i qRT-PCR analysis of thermogenic genes in BAT. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Indirect calorimetry (O 2 consumption, CO 2 production, RER, and heat production) was assessed using a Comprehensive Lab Animal Monitoring System (Columbus Instruments).

Techniques: Control, Staining, Quantitative RT-PCR

a Core body temperature recording in control and Orai1 BKO mice subjected to acute cold exposure (4 °C) for 6 hrs. b infrared thermography images upon cold exposure. c indirect calorimetry during cold exposure (n=6 mice per group). d Gross images of BAT of control and Orai1 BKO under room temperature and cold conditions. e H&E staining of BAT. f qRT-PCR of thermogenic gene expression in BAT. g Western blot analyses of lipolytic and thermogenic proteins. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: bioRxiv

Article Title: Orai1-mediated Ca 2+ Entry Regulates Lipolysis and Mitochondrial Activation in Brown Adipose Thermogenesis

doi: 10.64898/2026.04.24.718619

Figure Lengend Snippet: a Core body temperature recording in control and Orai1 BKO mice subjected to acute cold exposure (4 °C) for 6 hrs. b infrared thermography images upon cold exposure. c indirect calorimetry during cold exposure (n=6 mice per group). d Gross images of BAT of control and Orai1 BKO under room temperature and cold conditions. e H&E staining of BAT. f qRT-PCR of thermogenic gene expression in BAT. g Western blot analyses of lipolytic and thermogenic proteins. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Indirect calorimetry (O 2 consumption, CO 2 production, RER, and heat production) was assessed using a Comprehensive Lab Animal Monitoring System (Columbus Instruments).

Techniques: Control, Staining, Quantitative RT-PCR, Gene Expression, Western Blot