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Nascent RNA Florescence in-situ <t>hybridization</t> (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.
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Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.

Journal: STAR Protocols

Article Title: Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH

doi: 10.1016/j.xpro.2026.104441

Figure Lengend Snippet: Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.

Article Snippet: Humidified incubator for hybridization , Fisher Scientific , 15-015-2632.

Techniques: In Situ Hybridization, Staining