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<t>IHC</t> and RT-qPCR validation about significant hub genes. <t>Immunohistochemistry</t> images of KIF2C ( A ), PBK ( B ), TOP2A ( C ), CDK1 ( D ), and KIF20 ( E ) in normal and ATC tissues obtained from HPA database. The RT-qPCR was used to compare mRNA levels of KIF2C ( F ), PBK ( G ), TOP2A ( H ), CDK1 ( I ), KIF20A ( J ), and ASPM ( K ) in CAL-62 cells and Nthy-ori 3–1 cells. HPA, Human Protein Atlas; IHC, immunohistochemistry; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ATC, anaplastic thyroid carcinoma. (P values: ns, nonsignificant; *P < 0.05; **P < 0.01; ***P < 0.001)
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<t>IHC</t> and RT-qPCR validation about significant hub genes. <t>Immunohistochemistry</t> images of KIF2C ( A ), PBK ( B ), TOP2A ( C ), CDK1 ( D ), and KIF20 ( E ) in normal and ATC tissues obtained from HPA database. The RT-qPCR was used to compare mRNA levels of KIF2C ( F ), PBK ( G ), TOP2A ( H ), CDK1 ( I ), KIF20A ( J ), and ASPM ( K ) in CAL-62 cells and Nthy-ori 3–1 cells. HPA, Human Protein Atlas; IHC, immunohistochemistry; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ATC, anaplastic thyroid carcinoma. (P values: ns, nonsignificant; *P < 0.05; **P < 0.01; ***P < 0.001)
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<t>IHC</t> and RT-qPCR validation about significant hub genes. <t>Immunohistochemistry</t> images of KIF2C ( A ), PBK ( B ), TOP2A ( C ), CDK1 ( D ), and KIF20 ( E ) in normal and ATC tissues obtained from HPA database. The RT-qPCR was used to compare mRNA levels of KIF2C ( F ), PBK ( G ), TOP2A ( H ), CDK1 ( I ), KIF20A ( J ), and ASPM ( K ) in CAL-62 cells and Nthy-ori 3–1 cells. HPA, Human Protein Atlas; IHC, immunohistochemistry; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ATC, anaplastic thyroid carcinoma. (P values: ns, nonsignificant; *P < 0.05; **P < 0.01; ***P < 0.001)
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immunohistochemistry data human protein atlas (hpa) database - by Bioz Stars, 2026-09
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IHC and RT-qPCR validation about significant hub genes. Immunohistochemistry images of KIF2C ( A ), PBK ( B ), TOP2A ( C ), CDK1 ( D ), and KIF20 ( E ) in normal and ATC tissues obtained from HPA database. The RT-qPCR was used to compare mRNA levels of KIF2C ( F ), PBK ( G ), TOP2A ( H ), CDK1 ( I ), KIF20A ( J ), and ASPM ( K ) in CAL-62 cells and Nthy-ori 3–1 cells. HPA, Human Protein Atlas; IHC, immunohistochemistry; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ATC, anaplastic thyroid carcinoma. (P values: ns, nonsignificant; *P < 0.05; **P < 0.01; ***P < 0.001)

Journal: Discover Oncology

Article Title: Identification and validation of a novel prognostic signature and key genes related to development of anaplastic thyroid carcinoma

doi: 10.1007/s12672-024-01563-3

Figure Lengend Snippet: IHC and RT-qPCR validation about significant hub genes. Immunohistochemistry images of KIF2C ( A ), PBK ( B ), TOP2A ( C ), CDK1 ( D ), and KIF20 ( E ) in normal and ATC tissues obtained from HPA database. The RT-qPCR was used to compare mRNA levels of KIF2C ( F ), PBK ( G ), TOP2A ( H ), CDK1 ( I ), KIF20A ( J ), and ASPM ( K ) in CAL-62 cells and Nthy-ori 3–1 cells. HPA, Human Protein Atlas; IHC, immunohistochemistry; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; ATC, anaplastic thyroid carcinoma. (P values: ns, nonsignificant; *P < 0.05; **P < 0.01; ***P < 0.001)

Article Snippet: For further validation of the hub genes, the immunohistochemistry (IHC) database (The Human Protein Atlas, https://www.proteinatlas.org/ ) was also employed.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Immunohistochemistry, Reverse Transcription, Real-time Polymerase Chain Reaction