Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: In this study, we present the first evaluation of the recently FDA-cleared Borrelia B31 ViraChip IgM and IgG microarray immunoblot assays (ViraChip MIB assays; Viramed Biotech AG, Planegg, Germany), which were entirely automated on a Gemini Compact Microplate Processor (Stratec Biomedical AG, Birkenfeld, Germany).

Techniques: Control, Microarray, Western Blot

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: All included samples were tested within one freeze-thaw cycle by the following second-tier supplemental assays: the Borrelia B31 ViraChip IgM and IgG microarray immunoblots (ViraChip MIB assays; Viramed Biotech AG, Planegg, Germany), the Borrelia B31 ViraStripe IgM and IgG LIAs (Viramed Biotech AG, Planegg, Germany), and the MarDx B. burgdorferi IgM and IgG WB assays (Trinity Biotech, Carlsbad, CA).

Techniques: Control, Microarray, Western Blot

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Control, Microarray, Western Blot

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assays and ViraStripe LIA for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Comparison

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assay and MarDx WB assay for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Comparison, Western Blot

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip IgM and IgG MIB assays to a consensus standard for the presence or absence of IgM and IgG antibodies to B. burgdorferi

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Comparison

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Performance of the ViraStripe LIA and ViraChip MIB assay in clinically characterized sera

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Control

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Total processing times for the ViraChip MIB and ViraStripe LIA for a full batch of patient samples and controls per assay

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques: Microarray, Western Blot

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assays and ViraStripe LIA for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assay and MarDx WB assay for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques: Western Blot

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip IgM and IgG MIB assays to a consensus standard for the presence or absence of IgM and IgG antibodies to B. burgdorferi

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Performance of the ViraStripe LIA and ViraChip MIB assay in clinically characterized sera

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Total processing times for the ViraChip MIB and ViraStripe LIA for a full batch of patient samples and controls per assay

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques: