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Multiplex protein profiling <t>of</t> <t>CTCs</t> and LEVs. ( A , B ) Example of IF (top panels) and <t>IMC</t> (bottom panels) images of a CTC ( A ) and a LEV ( B ). The arrow points at the LEV which is negative for DAPI and weakly stained by the IMC DNA intercalator. Scale bar is 10 µm. ( C ) Heatmap of IMC scores on a 0–3 scale with 0 = white and dark blue = 3, ordered by object categories and patients, and clustered by proteins.
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Image Search Results


Multiplex protein profiling of CTCs and LEVs. ( A , B ) Example of IF (top panels) and IMC (bottom panels) images of a CTC ( A ) and a LEV ( B ). The arrow points at the LEV which is negative for DAPI and weakly stained by the IMC DNA intercalator. Scale bar is 10 µm. ( C ) Heatmap of IMC scores on a 0–3 scale with 0 = white and dark blue = 3, ordered by object categories and patients, and clustered by proteins.

Journal: Cancers

Article Title: Large Extracellular Vesicle Characterization and Association with Circulating Tumor Cells in Metastatic Castrate Resistant Prostate Cancer

doi: 10.3390/cancers13051056

Figure Lengend Snippet: Multiplex protein profiling of CTCs and LEVs. ( A , B ) Example of IF (top panels) and IMC (bottom panels) images of a CTC ( A ) and a LEV ( B ). The arrow points at the LEV which is negative for DAPI and weakly stained by the IMC DNA intercalator. Scale bar is 10 µm. ( C ) Heatmap of IMC scores on a 0–3 scale with 0 = white and dark blue = 3, ordered by object categories and patients, and clustered by proteins.

Article Snippet: CTCs and LEVs confirmed by 40× imaging were relocated in the Hyperion IMC instrument (Fluidigm, South San Francisco, CA, USA) using a defined offset between coordinates of the 80i microscope (Nikon, Melville, NY, USA) and the Hyperion stage.

Techniques: Multiplex Assay, Staining

Biomarker expression in CTCs and LEVs of AVPC. ( A ) Example of HSPD1 expression in LEV (top panel), apoptotic (middle panel) and CTC (bottom panel). Cropped ROI images are shown with individual IF channels and IF composite image with DAPI (blue), CD45 (green) and pan-CK (red). The IMC rendered image of HSPD1 signal is shown in green. vs. CD45 for all objects within the full ROI with WBCs in purple and the cell of interest (circled) in red (full cell), green (cytoplasm) and teal (nucleus). ( B ) Selected biomarkers shown for LEVs (top 2 panels) and CTCs (bottom 2 panels), with EpCAM expression varying from negative (0) to strong (3+). Scores from the 0–3 scale is shown for each marker and object. Scale bar is 10 µm.

Journal: Cancers

Article Title: Large Extracellular Vesicle Characterization and Association with Circulating Tumor Cells in Metastatic Castrate Resistant Prostate Cancer

doi: 10.3390/cancers13051056

Figure Lengend Snippet: Biomarker expression in CTCs and LEVs of AVPC. ( A ) Example of HSPD1 expression in LEV (top panel), apoptotic (middle panel) and CTC (bottom panel). Cropped ROI images are shown with individual IF channels and IF composite image with DAPI (blue), CD45 (green) and pan-CK (red). The IMC rendered image of HSPD1 signal is shown in green. vs. CD45 for all objects within the full ROI with WBCs in purple and the cell of interest (circled) in red (full cell), green (cytoplasm) and teal (nucleus). ( B ) Selected biomarkers shown for LEVs (top 2 panels) and CTCs (bottom 2 panels), with EpCAM expression varying from negative (0) to strong (3+). Scores from the 0–3 scale is shown for each marker and object. Scale bar is 10 µm.

Article Snippet: CTCs and LEVs confirmed by 40× imaging were relocated in the Hyperion IMC instrument (Fluidigm, South San Francisco, CA, USA) using a defined offset between coordinates of the 80i microscope (Nikon, Melville, NY, USA) and the Hyperion stage.

Techniques: Biomarker Discovery, Expressing, Marker