imaging mass cytometry imc data (fluidigm)
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imaging mass cytometry imc data
Imaging Mass Cytometry Imc Data, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imc+data/Hyperion+Imaging+Mass+Cytometry+System/pmc12069457-68-0-12
Average 93 stars, based on 1523 article reviews
Imaging Mass Cytometry Imc Data, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imc+data/Hyperion+Imaging+Mass+Cytometry+System/pmc12069457-68-0-12
Average 93 stars, based on 1523 article reviews
imaging mass cytometry imc data - by Bioz Stars,
2026-10
93/100 stars
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Imaging:Article Title: Aptamer Probes Labeled with Lanthanide‐Doped Carbon Nanodots Permit Dual‐Modal Fluorescence and Mass Cytometric Imaging Article Snippet: .. The Article Title: Zr-NMOF tagged with heterobifunctionalized aptamers for highly sensitive, multiplexed and rapid imaging mass cytometry. Article Snippet: Imaging mass cytometry (IMC) permits high-dimensional single-cell spatial proteomics by harnessing mass tags to replace conventional fluorescence tags.. However, the current IMC technique commonly adopts metal-chelated polymer (MCP) tags, which are limited in sensitivity, multiplicity and data acquisition speed.. Here, we demonstrate nanometal–organic framework (NMOF) tags, which could concurrently augment IMC’s sensitivity, multiplicity, and acquisition speed. Article Title: High-multiplex single-cell imaging analysis reveals tumor immune contexture associated with clinical outcomes after CAR T cell therapy. Article Snippet: 1Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2Immunotherapy Research Center for Hematologic Diseases of Hubei Province, Wuhan 430030, China; 3Department of Hematology, Renmin Hospital of Wuhan University, Wuhan 430060, China; 4Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin 150010, China; 5Department of Hematology, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150081, China; 6Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 7Biomedical Research Core Facilities, Westlake University, Hangzhou 310024, China; 8Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan 430030, China; 9Research Institute of Huazhong University of Science and Technology in Shenzhen, Shenzhen 518000, Article Title: Extracellular matrix phenotyping by imaging mass cytometry defines distinct cellular matrix environments associated with allergic airway inflammation Article Snippet: .. Article Title: FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice. Article Snippet: Finally, IMC data corresponding to pre-established regions of interest (ROIs) were acquired as raw .mcd files with the Hyperion imaging system (Fluidigm). .. Finally, Article Title: High-content multimodal analysis supports the IL-7/IL-7 receptor axis as a relevant therapeutic target in primary Sjögren's syndrome. Article Snippet: Objective: While the involvement of IL-7/IL-7R axis in pSS has been described in relation to T cells, little is known about the contribution of this pathway in relationship with other immune cells, and its implication in autoimmunity.. Using high-content multiomics data, we aimed at characterizing IL-7R expressing cells and the involvement of IL-7/IL-7R pathway in pSS pathophysiology.. Methods: An IL-7 signature established using RNA-sequencing of human PBMCs incubated with IL-7 was applied to 304 pSS patients, and on RNA-Seq datasets from tissue biopsies. Article Title: Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis. Article Snippet: Following antibody incubation, slides were washed with PBS and stained with Cell-IDTM Intercalator-Ir (Standard BioTools Inc.) and OsO4 (Sigma-Aldrich) in PBS for 2—3 h to label DNA and lipids, respectively, then washed with distilled, deionized water twice for 5 min before air-drying. .. Software:Article Title: Aptamer Probes Labeled with Lanthanide‐Doped Carbon Nanodots Permit Dual‐Modal Fluorescence and Mass Cytometric Imaging Article Snippet: .. The Article Title: Zr-NMOF tagged with heterobifunctionalized aptamers for highly sensitive, multiplexed and rapid imaging mass cytometry. Article Snippet: Imaging mass cytometry (IMC) permits high-dimensional single-cell spatial proteomics by harnessing mass tags to replace conventional fluorescence tags.. However, the current IMC technique commonly adopts metal-chelated polymer (MCP) tags, which are limited in sensitivity, multiplicity and data acquisition speed.. Here, we demonstrate nanometal–organic framework (NMOF) tags, which could concurrently augment IMC’s sensitivity, multiplicity, and acquisition speed. Marker:Article Title: High-multiplex single-cell imaging analysis reveals tumor immune contexture associated with clinical outcomes after CAR T cell therapy. Article Snippet: 1Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2Immunotherapy Research Center for Hematologic Diseases of Hubei Province, Wuhan 430030, China; 3Department of Hematology, Renmin Hospital of Wuhan University, Wuhan 430060, China; 4Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin 150010, China; 5Department of Hematology, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150081, China; 6Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 7Biomedical Research Core Facilities, Westlake University, Hangzhou 310024, China; 8Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan 430030, China; 9Research Institute of Huazhong University of Science and Technology in Shenzhen, Shenzhen 518000, Expressing:Article Title: High-multiplex single-cell imaging analysis reveals tumor immune contexture associated with clinical outcomes after CAR T cell therapy. Article Snippet: 1Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2Immunotherapy Research Center for Hematologic Diseases of Hubei Province, Wuhan 430030, China; 3Department of Hematology, Renmin Hospital of Wuhan University, Wuhan 430060, China; 4Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin 150010, China; 5Department of Hematology, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin 150081, China; 6Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 7Biomedical Research Core Facilities, Westlake University, Hangzhou 310024, China; 8Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan 430030, China; 9Research Institute of Huazhong University of Science and Technology in Shenzhen, Shenzhen 518000, Tissue:Article Title: Dark Microglia Are Abundant in Normal Postnatal Development, where they Remodel Synapses via Phagocytosis and Trogocytosis, and Are Dependent on TREM2 Article Snippet: Flash-frozen, 6μm mouse sections underwent IMC staining using an adapted protocol from manufacturer (Standard BioTools). .. Cytometry:Article Title: High-content multimodal analysis supports the IL-7/IL-7 receptor axis as a relevant therapeutic target in primary Sjögren's syndrome. Article Snippet: Objective: While the involvement of IL-7/IL-7R axis in pSS has been described in relation to T cells, little is known about the contribution of this pathway in relationship with other immune cells, and its implication in autoimmunity.. Using high-content multiomics data, we aimed at characterizing IL-7R expressing cells and the involvement of IL-7/IL-7R pathway in pSS pathophysiology.. Methods: An IL-7 signature established using RNA-sequencing of human PBMCs incubated with IL-7 was applied to 304 pSS patients, and on RNA-Seq datasets from tissue biopsies. Article Title: Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis. Article Snippet: Following antibody incubation, slides were washed with PBS and stained with Cell-IDTM Intercalator-Ir (Standard BioTools Inc.) and OsO4 (Sigma-Aldrich) in PBS for 2—3 h to label DNA and lipids, respectively, then washed with distilled, deionized water twice for 5 min before air-drying. .. |
