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Cell migration (wound healing) assay in HeLa, UD-SCC-2, and VX2 cells. HeLa ( A ), UD-SCC-2 ( B ), and VX2 ( C ) tumor cells were treated with photodynamic therapy (PDT) only, curcumin liposomes (Cur lipo) only, or Cur lipo in combination with PDT. Images were captured at t = 0 h directly after scratching the cell layer and t = 24 h to evaluate scratch (wound) closure indicative of cellular migration. The cell-free area of the scratched region was measured with the Montpellier Ressources Imagerie (MRI) wound healing tool used with the ImageJ analysis software . The level of cell migration is presented as the percentage of scratch (wound) closure observed 24 h after treatment compared to control values. Controls indicate untreated cells. The values are expressed as the mean ± standard deviation, and statistical significances are indicated as *** p < 0.001, ** p < 0.01.

Journal: Cancers

Article Title: Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes

doi: 10.3390/cancers12113278

Figure Lengend Snippet: Cell migration (wound healing) assay in HeLa, UD-SCC-2, and VX2 cells. HeLa ( A ), UD-SCC-2 ( B ), and VX2 ( C ) tumor cells were treated with photodynamic therapy (PDT) only, curcumin liposomes (Cur lipo) only, or Cur lipo in combination with PDT. Images were captured at t = 0 h directly after scratching the cell layer and t = 24 h to evaluate scratch (wound) closure indicative of cellular migration. The cell-free area of the scratched region was measured with the Montpellier Ressources Imagerie (MRI) wound healing tool used with the ImageJ analysis software . The level of cell migration is presented as the percentage of scratch (wound) closure observed 24 h after treatment compared to control values. Controls indicate untreated cells. The values are expressed as the mean ± standard deviation, and statistical significances are indicated as *** p < 0.001, ** p < 0.01.

Article Snippet: ImageJ analysis software [ ] (MRI_wound healing tool, written by V. Baecker; collaborators: N. Cahuzac and V. Georget; (c) 2010-2017, INSERM) was used to quantify the area of the scratch.

Techniques: Migration, Wound Healing Assay, Liposomes, Software, Control, Standard Deviation