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Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.
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Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.
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Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.
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Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.
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image-pro plus morphometric software  (Morrell Instrument Company Inc)
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Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.
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Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.

Journal: Journal of Food and Drug Analysis

Article Title: Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy

doi: 10.1016/j.jfda.2015.08.006

Figure Lengend Snippet: Oyster-derived polysaccharides (OPS) restored the diminished ratio of villus length over crypt depth in the duodenum. Mice were treated as described in . Tissue sections of duodenum were prepared for standard hematoxylineosin (H&E) staining. The villus length and crypt depth were measured using the Image-Pro Plus version 5.1 morphometric analysis software (100× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 27-1 samples per group pooled from six independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin.

Article Snippet: The number of IL-4 + cells (the red color) was identified in contrast to the background counter stain (the blue color) using Image-Pro Plus version 5.1 morphometric analysis software (Media Cybernetics, Inc.).

Techniques: Derivative Assay, Staining, Software

Effect of oyster-derived polysaccharides (OPS) on the infiltration of interleukin-4 (IL-4) + cells in duodenum. Mice were treated as described in . Immunohistochemical (IHC) staining was performed to detect IL-4 + cells in the duodenum. The image was analyzed and the positive signal (the red color) was quantified using the Image-Pro Plus version 5.1 morphometric analysis software (200× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 20–22 samples per group pooled from four independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin; VH = vehicle.

Journal: Journal of Food and Drug Analysis

Article Title: Pacific oyster-derived polysaccharides attenuate allergen-induced intestinal inflammation in a murine model of food allergy

doi: 10.1016/j.jfda.2015.08.006

Figure Lengend Snippet: Effect of oyster-derived polysaccharides (OPS) on the infiltration of interleukin-4 (IL-4) + cells in duodenum. Mice were treated as described in . Immunohistochemical (IHC) staining was performed to detect IL-4 + cells in the duodenum. The image was analyzed and the positive signal (the red color) was quantified using the Image-Pro Plus version 5.1 morphometric analysis software (200× field; Media Cybernetics, Inc., Silver Spring, MD, USA). Data are expressed as the mean ± standard error of 20–22 samples per group pooled from four independent experiments. * p < 0.05 compared to the VH group [vehicle-treated (distilled water; 0.1 mL/mouse)]. NS = nonsensitized; OVA = ovalbumin; VH = vehicle.

Article Snippet: The number of IL-4 + cells (the red color) was identified in contrast to the background counter stain (the blue color) using Image-Pro Plus version 5.1 morphometric analysis software (Media Cybernetics, Inc.).

Techniques: Derivative Assay, Immunohistochemical staining, Immunohistochemistry, Software