Journal: Bioactive Materials

Article Title: Energetic metabolism-regulatory glycopeptide hydrogel accelerates pressure ulcer wound repair

doi: 10.1016/j.bioactmat.2026.02.016

Figure Lengend Snippet: GMI gel -mediated metabolic reprogramming and its regulatory role in macrophage polarization. A-D) Hexokinase activity, Phosphofructokinase activity, Isocitrate dehydrogenase activity, and Succinate dehydrogenase activity, n = 3. E-H) Heatmap of LC-MS data and quantitative analysis of the relative abundance of glycolysis and TCA cycle metabolites, n = 3. I) Schematic diagram of glycolysis and the TCA cycle. J) Representative CLSM images of RAW 264.7 cells treated with GMI gel for 72 h. K) Flow cytometry analysis of CD206 and CD86 expression. L, M) The secretion of IL-6 and TNF-α in the culture supernatant of RAW264.7 cells, n = 3. Data are shown as mean ± SDs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant (p > 0.05).

Article Snippet: The expression levels of IL-6 and TNF-α in the culture medium were measured using commercial ELISA kits (Cusabio, Wuhan, China).

Techniques: Activity Assay, Liquid Chromatography with Mass Spectroscopy, Flow Cytometry, Expressing

Journal: Bioactive Materials

Article Title: Energetic metabolism-regulatory glycopeptide hydrogel accelerates pressure ulcer wound repair

doi: 10.1016/j.bioactmat.2026.02.016

Figure Lengend Snippet: GMI gel promotes the healing of infected pressure ulcers in vivo. A) Schematic diagram of GMI gel treatment of infected pressure ulcers. B) Photographs of wounds in mice at different treatment times. C) Signs of wound closure. D) Wound size at different treatment times, n = 3. E) H&E staining images of mouse wound tissue after different treatments on day 12. F) Masson staining images of mouse wound tissue after different treatments on day 12. G) Representative laser Doppler perfusion images of wounds in mice in each treatment group on day 12. H) Representative images of immunohistochemical staining for TNF- α, IL-6 and IL-10 12 days after treatment. I-L) Quantitative statistics of wound site blood perfusion, TNF- α, IL-6 and IL-10, n = 3. Data are shown as mean ± SDs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The expression levels of IL-6 and TNF-α in the culture medium were measured using commercial ELISA kits (Cusabio, Wuhan, China).

Techniques: Infection, In Vivo, Staining, Immunohistochemical staining

Journal: Scientific Reports

Article Title: Aescin and rutin mitigate hyperthyroidism-induced multisystem dysfunction via antioxidant and anti-inflammatory mechanisms in a rat model

doi: 10.1038/s41598-026-46124-6

Figure Lengend Snippet: ( A – B ) Effects of Propylthiouracil (PTU), rutin, and aescin on serum inflammatory markers in hyperthyroid-induced male rats. (A) Serum interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) levels. Data are presented as mean ± standard error; different superscript letters within the same column are regarded as substantially significant ( P ≤ 0.05).

Article Snippet: Serum levels of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), dopamine (DA), and activity of acetylcholinesterase (AChE) were measured using rats’ reagent (ELISA) kits (CSB-E11987r, CSB-E04640r, CSB-E08660r, KT-5344, respectively), obtained from Cusabio Biotech Co., China, and Kamiya Biomedical Co., USA.

Techniques:

Journal: bioRxiv

Article Title: Heterogeneous pro-inflammatory response to BRAFV600E-induced thyroid tumor development

doi: 10.64898/2026.03.26.714444

Figure Lengend Snippet: Comparative qPCR data analyzed in tissue samples obtained from non-induced TgCreER T2 ;Braf V600E/+ mice at 3 and 6 months of age (whole lobes) and 12 months old mutants (excised tumors). For all experiment, transcript expression levels were compared to that of control thyroids obtained from age-matched non-mutant (wt) mice. A Heterogeneous expression of Il1b , Il6 and Tnfa in thyroids of individual mice. Order of presentation deduced by the ranked expression levels of Il1b increasing from left to right (n=8) for both time points. . B-C Heterogeneous cytokine expression in Braf CA -induced papillary thyroid carcinoma of different clonal origin. Macroscopically discernable tumors (n=4) were excised from a single mutant thyroid specimen (B, thyroid gland in situ (outlined) to the left; cartoon indicating dissected tumor borders to the right) and processed for qPCR analysis of Il1b and Il6 . Tg transcripts were simultaneously measured for correlation to level of Braf CA -induced thyroid dedifferentiation.

Article Snippet: Primers to mouse Tg (forward: 5′-CATGGAATCTAATGCCAAGAACTG-3′; reverse: 5′-TCCCTGTGAGCTTTTGGAATG-3′), Tpo (forward: 5′-CAAAGGCTGGAACCCTAATTTCT-3′; reverse: 5′-AACTTGAATGAGGTGCCTTGTCA-3′), Tshr (forward: 5′-TCCCTGAAAACGCATTCCA-3′; reverse: 5′-GCATCCAGCTTTGTTCCATTG-3′), Slc5a5 (forward: 5′-TCCACAGGAATCATCTGCACC-3′; reverse: 5′-CCACGGCCTTCATACCACC-3′), Pax8 (forward: 5′-GATAGGAGACTACAAGCGGCA-3′); reverse:5′CGGATGATTCTGTTGATGGAGC-3′), Il1b (forward: 5′-CCTTCCAGGATGAGGACATGA-3′; reverse: 5′-TGAGTCACAGAGGATGGGCTC- 3′), Il6 (forward: 5′-GATACCACTCCCAACAGACCT-3′; reverse: 5′-CTCATTTCCACGATTTCCCAGA-3′), Tnfa (forward: 5′- TGCCTATGTCTCAGCCTCT-3′; reverse: 5′-GAGGCCATTTGGGAACTTCT-3′) - were designed in Primer-BLAST ( ) based on sequences from public databases (Ensembl or Santa Cruz Genome Browser).

Techniques: Expressing, Control, Mutagenesis, In Situ