Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: RT-qPCR analysis of DNMTs and IL1R2 expression, along with IHC staining (A) RT-qPCR was used to quantitatively analyze the expression profiles of DNMTs and IL1R2 in HBW and LBW placentas ( DNMT3A : p = 0.0264; DNMT3L : p = 0.0016; DNMT1 : p = 0.0069; IL1R2 : p = 0.0156). (B and D) IHC was performed to analyze the protein expression intensity of DNMT3A in HBW and LBW placentas (DNMT3A: p = 0.0059). Scale bars, 50 μm. (C and E) IHC was performed to analyze the protein expression intensity of IL1R2 in HBW and LBW placentas (IL1R2: p = 0.0007). Scale bars, 50 μm. Data are represented as mean ± SEM. Statistical significance was assessed by paired Student’s t test, with n = 6 (3 samples in the LBW group and 3 samples in the HBW group).

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemistry

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: MethPrimer-based prediction of CpG islands in the IL1R2 promoter region and subsequent analysis of the methylation status in placental tissues (A) Bioinformatics analysis predicted CpG islands in the promoter region of IL1R2 . (B) MS-PCR measured IL1R2 methylation levels in HBW and LBW placentas ( p = 0.0062). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, with n = 6 (3 samples in the LBW group and 3 samples in the HBW group).

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Methylation

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: Effects of 5-Aza treatment on IL1R2 gene expression and methylation levels in PTr2 cells (A) The mRNA expression of IL1R2 in 5-Aza-treated PTr2 cells was measured using RT-qPCR (20 μM: p = 0.0199). “ns” denotes non-significant results ( p > 0.05). (B) MS-PCR measured the methylation levels of the IL1R2 promoter in 5-Aza-treated PTr2 Cells ( p = 0.0005). (C) BS-PCR measured the methylation levels of the IL1R2 promoter in 5-Aza-treated PTr2 cells ( p = 0.0011). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Gene Expression, Methylation, Expressing, Quantitative RT-PCR

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 knockdown promotes PTr2 Cell proliferation and migration (A) RT-qPCR analysis of IL1R2 knockdown efficiency and its effect on proliferation and apoptosis markers in PTr2 cells ( IL1R2 : p = 0.0002; Ki67 : p = 0.0043; BAX : p = 0.0155; CASP3 : p = 0.0088; CASP9 : p = 0.0331). “ns” denotes non-significant results ( p > 0.05). (B) Cell proliferation assessed using CCK-8 assay after IL1R2 knockdown (48 h: p = 0.0224; 72 h: p = 0.0035). (C and D) Cell proliferation evaluated using EdU assay ( p = 0.0083). Scale bars, 100 μm. (E and F) Cell migration was analyzed by scratch assay ( p = 0.0462). Scale bars, 500 μm. Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Knockdown, Migration, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 overexpression inhibits PTr2 Cell proliferation and migration (A) RT-qPCR analysis of IL1R2 overexpression efficiency and its effect on proliferation and apoptosis markers in PTr2 cells ( IL1R2 : p = 0.0003; PCNA : p = 0.0256; BAX : p = 0.0497). “ns” denotes non-significant results ( p > 0.05). (B) Cell proliferation was assessed using CCK-8 assay following IL1R2 overexpression (48 h: p = 0.0027; 72 h: p = 0.0011). (C and D) Cell proliferation evaluated using EdU assay ( p = 0.0452). Scale bars, 100 μm. (E and F) Cell migration was analyzed by scratch assay ( p = 0.0078). Scale bars, 500 μm. Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Over Expression, Migration, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 positively regulates TNF- α expression in PTr2 cells (A) IL1R2 knockdown suppresses TNF-α mRNA levels ( p = 0.0442). (B) IL1R2 overexpression elevates TNF-α mRNA levels ( p = 0.0057). (C - E) IL1R2 knockdown reduces TNF- α protein abundance, with confirmation of knockdown efficiency (IL1R2: p = 0.0012; TNF-α: p = 0.0394). (F - H) IL1R2 overexpression increases TNF- α protein levels, with confirmation of knockdown efficiency (IL1R2: p = 0.0011; TNF-α: p = 0.0014). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Expressing, Knockdown, Over Expression, Quantitative Proteomics

Journal: bioRxiv

Article Title: Inflammatory IL-1 signaling remodels epidermal stem cell compartments by suppressing Wnt activity

doi: 10.64898/2026.02.06.704488

Figure Lengend Snippet: ( A ) Experimental scheme of IL-1-induced skin inflammation. Mouse tail skin was intradermally (ID) injected with 0.1% BSA (vehicle), IL-1α, or IL-1β once daily for 3 consecutive days. ( B–D ) Hematoxylin and eosin staining of sagittal sections of tail skin (B). Scale bars: 300 µm. Epidermal thickness was measured in the interscale and scale regions (C, D). ( E–G ) Whole-mount staining of K10 (interscale) and K36 (scale), and their quantifications. Scale bars: 100 µm. ( H ) Strategy for overexpressing Il1r2 in pTRE-Il1r2-Myc/Rosa-rtTA transgenic mice (Il1r2 Tg). ( I ) Experimental scheme of Il1r2 overexpression. Il1r2 Tg mice aged 2–3 months were fed doxycycline chow for 7 days before the procedures and maintained on the same diet for the indicated durations. Mouse tail skin was treated with vehicle (EtOH) or TPA on days 7 and 9. ( J ) Il1r2 expression in whole tail skin, measured by qRT–PCR. ( K, L ) Section immunostaining of Il1r2 and K36 (scale) and its quantifications (L). The yellow arrows indicate Il1r2+ cells. Scale bars: 50 µm. The number of Il1r2+ cells per one structure. ( M, N ) Hematoxylin and eosin staining of sagittal sections of tail skin. Scale bars: 100 µm. Epidermal thickness was measured in the interscale and scale regions (N). ( O, P ) Whole-mount staining of K10 (interscale) and K36 (scale), and their quantifications (P). Scale bars: 100 µm. All data are presented as the mean ± SD. Each dot represents an independent biological replicate. Statistical significance was assessed using two-way ANOVA for (C, D), one-way ANOVA for (L, N, P), and a two-tailed t test (F, G, J). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant.

Article Snippet: Genomic integration was confirmed by PCR with pTRE-Il1r2 transgene-specific primers (F 5′-TCGCCTGGAGACGCCAT-3′, R 5′-ACTGACAGTTGTCTCCATGGA-3′). pTRE-Il1r2 mice were crossed with Rosa-rtTA mice (JAX no. 005670).

Techniques: Injection, Staining, Transgenic Assay, Over Expression, Expressing, Quantitative RT-PCR, Immunostaining, Two Tailed Test

Journal: bioRxiv

Article Title: Inflammatory IL-1 signaling remodels epidermal stem cell compartments by suppressing Wnt activity

doi: 10.64898/2026.02.06.704488

Figure Lengend Snippet: ( A, B ) Expression of inflammatory cytokines in the whole tail skin of WT mice injected with IL-1α (A), or IL-1β (B) once daily for 3 consecutive days, measured by qRT-qPCR. ( C ) Expression of inflammatory cytokines in the whole tail skin of pTRE-Il1r2-Myc/Rosa-rtTA (Il1r2 Tg) or control (Ctrl) mice treated twice with ETOH or TPA, measured by qRT–PCR. All data are presented as the mean ± SD. Each dot represents an independent biological replicate. Statistical significance was assessed using a two-tailed t test for (A, B) and one-way ANOVA for (C). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant.

Article Snippet: Genomic integration was confirmed by PCR with pTRE-Il1r2 transgene-specific primers (F 5′-TCGCCTGGAGACGCCAT-3′, R 5′-ACTGACAGTTGTCTCCATGGA-3′). pTRE-Il1r2 mice were crossed with Rosa-rtTA mice (JAX no. 005670).

Techniques: Expressing, Injection, Control, Quantitative RT-PCR, Two Tailed Test

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: IL1R2 as a marker for tiTregs. A, Percentage of IL1R2 + cells (as measured via flow cytometry) within the tiTregs infiltrating different murine tumor models in WT mice ( n = 3–7, where n refers to the number of animals). B, Percentage of IL1R2 + cells within the Tregs present in various organs of naïve ( n = 4–10), E0771-bearing ( n = 4–10), or MC38-bearing ( n = 4–10) mice. C and D, Seventeen thousand sixty-seven CD45 + cells from E0771 tumors, profiled by CITE-seq ( C ) and 26,814 CD45 + cells from MC38 tumors, profiled by scRNA-seq ( D ). Left, UMAP plot with each cell cluster receiving a different color; middle, UMAP feature plot, with color reflecting the Il1r2 expression level in each cluster; right, dot plot with the color indicating the average Il1r2 expression and the size showing the percentage of cells that expresses Il1r2 . E, Percentage of IL1R2 + cells within the different cell populations infiltrating E0771 ( n = 5) and MC38 ( n = 4) tumors grown in WT mice. For A , B , and E , data are shown as mean ± SEM. For B , one-way ANOVA for comparison of every organ with tumor (****, P ≤ 0.0001 over tumor bar) and two-way ANOVA for comparison of tumor-bearing vs. naïve organ. **, P ≤ 0.01; ****, P ≤ 0.0001. Mac, macrophages; migDC, migratory dendritic cell; MoDC, monocyte-derived dendritic cell; Mono, monocytes; prolif, proliferation; pDC, plasmacytoid dendritic cell; TAM, tumor-associated macrophages.

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Marker, Flow Cytometry, Expressing, Comparison, Derivative Assay

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: tiTreg subsets in human TNBC and colorectal carcinoma. A, UMAP plot of the subclustering of 793 tiTregs from TNBC tumors (scRNA-seq). B, Dot plot of marker gene expression of tiTreg clusters infiltrating TNBC tumors. C, UMAP plot of the subclustering of 1,063 tiTregs from colorectal carcinoma (CRC) tumors (scRNA-seq). D, Dot plot of marker gene expression of tiTreg clusters infiltrating colorectal carcinoma tumors. In B and D , the color indicates the average gene expression and the size shows the percentage of cells that expresses the gene. E, Scatter plot, comparing the log P value of the differentially expressed genes in IL1R2-hi REL-hi vs. IL1R2-neg human TNBC Tregs and in Il1r2-hi Rel-hi vs. Il1r2-neg mouse E0771 Tregs. F, Scatter plot, comparing the log P value of the differentially expressed genes in IL1R2-hi REL-hi vs. IL1R-neg human colorectal carcinoma Tregs and in Il1r2-hi Rel-hi vs. Il1r2-neg mouse MC38 Tregs. The sign of the log P value corresponds to upregulation or downregulation of genes, respectively. Commonly upregulated or downregulated genes are labeled in red and blue, respectively. Genes that are only upregulated or downregulated within one species (human or mouse) are labeled in magenta. P value adjustment was performed using Bonferroni correction.

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Marker, Gene Expression, Labeling

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: Phenotypical and functional characterization of tiTregs. A and B, ΔMFI of different activation markers (CD25, CCR8, ICOS, Helios, and PD-1) within tiTregs infiltrating E0771 ( n = 5; A ), or MC38 ( n = 4; B ) tumors. Data are shown as mean ± SEM. Unpaired Student t test. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. C, Half-offset view of histogram plots of the CFSE signal of CD8 + T cells, cocultured with/without anti-CD3/anti-CD28, and in different ratios with IL1R2 + or IL1R2 − tiTregs sorted from a pool of E0771 tumors. Data shown are representative of two independent experiments.

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Functional Assay, Activation Assay

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: Regulation of IL1R2 upregulation on tiTregs. A–C, Percentage IL1R2 + cells within splenic WT ( n = 3; A ) or OT-II ( n = 3) Tregs ( A and B ), or within WT or OT-II CD4 + Foxp3 − T cells ( n = 3; C ) before/after 24 hours of stimulation with/without anti-CD3/anti-CD28, ovalbumin, and/or IL1β. D, Percentage of IL1R2 + cells within CellTrace + splenic WT or OT-II Tregs, injected into LLC ( n = 3) or LLC-OVA ( n = 3) tumors. E, Percentage of tiTregs within CD45 + cells and percentage of IL1R2 + cells within tiTregs infiltrating E0771 tumors grown in Il1r1 −/− ( n = 3) and their WT littermate control ( n = 3) mice. F and G, Heatmap showing the average regulon activity in E0771 ( F ) and MC38 ( G ) tiTreg clusters, based on SCENIC analysis. The regulon activity is calculated per cell, scaled by gene and averaged per cluster. Selected regulons with specific activity in the Il1r2 -hi tiTregs based on the RSS. H, Percentage of IL1R2 + Tregs and live Tregs within the total splenic Tregs retrieved from WT mice ( n = 4) after 24 hours of in vitro incubation with anti-CD3/anti-CD28 and increasing concentrations of the c-Rel inhibitor pentoxifylline (PTX). For A–E and H , data are shown as mean ± SEM. For A and C , two-way ANOVA, compared with 24 hours of incubation without additions. For B and D , one-way ANOVA. For E , unpaired Student t test. For H , two-way ANOVA. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, not significant.

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Injection, Control, Activity Assay, In Vitro, Incubation

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: IL1R2 is functionally redundant on tiTregs. A and B, Average growth curves ( A ) and weights ( B ) of MC38 tumors in IL1R2 Δtreg ( n = 7) and littermate (LT) control ( n = 5) mice. C, Percentage of tiTregs within CD45 + cells of MC38 tumors in IL1R2 Δtreg ( n = 7) and LT control ( n = 4) mice. D, ΔMFI of activation markers (CD25, CCR8, Helios, and PD-1) on MC38 tiTreg from IL1R2 Δtreg ( n = 7) and LT control ( n = 4) mice. E and F, Average growth curves ( E ) and weights ( F ) of E0771 tumors in Il1r2 −/− ( n = 7) and WT LT control ( n = 5) mice. G, Percentage of tiTregs within CD45 + cells of E0771 tumors in Il1r2 −/− ( n = 7) and WT LT control ( n = 5) mice. H, Percentages of immune cells within the CD45 + compartment of E0771 tumors in Il1r2 −/− ( n = 7) and WT LT control ( n = 5) mice. I, ΔMFI of activation markers (CD25, CCR8, Helios, and ICOS) on E0771 tiTreg from Il1r2 −/− ( n = 7) and their WT LT control ( n = 5) mice. J and K, Average growth curves ( J ) and weights ( K ) of MC38 tumors in Il1r2 −/− ( n = 4) and WT LT control ( n = 6) mice. L, Percentage of tiTregs within CD45 + cells of MC38 tumors in Il1r2 − /− ( n = 4) and WT LT control ( n = 6) mice. M, Percentages of immune cells within the CD45 + compartment of MC38 tumors in Il1r2 −/− ( n = 4) and WT LT control ( n = 6) mice. N, ΔMFI of activation markers (CD25, CCR8, Helios, and ICOS) on MC38 tiTreg from Il1r2 −/− ( n = 4) and WT LT control ( n = 6) mice. Data are shown as mean ± SEM. For A , E , H , J , and M , two-way ANOVA. For B–D , F , G , I , K , L , and N , unpaired Student t test. *, P ≤ 0.05; ****, P ≤ 0.0001. MoDC, monocyte-derived dendritic cell; TAM, tumor-associated macrophages.

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Control, Activation Assay, Derivative Assay

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: Monotherapy with different anti-IL1R2 Nb-Fc constructs in E0771 and MC38 tumor-bearing mice. A, Overview of the different anti-IL1R2 Nb-Fc constructs. B and C, Average growth curves of E0771 ( n = 10; B ) and MC38 ( n = 10; C ) tumors in WT mice treated with anti-IL1R2 Nb-Fc constructs. Data are shown as mean ± SEM. Statistics: two-way ANOVA. D and E, Kaplan–Meier survival curves of WT mice bearing E0771 ( n = 10; D ) or MC38 ( n = 10; E ) tumors, which were sacrificed when tumors reached humane endpoint. F and G, Percentage of IL1R2 + tiTregs within tiTregs, tiTregs, cDC1s, cDC2s, neutrophils, and CD8 + T cells within CD45 + cells of E0771 ( F ) and MC38 ( G ) tumors grown in WT mice treated with anti-IL1R2 Nb-Fc constructs. Data are shown as mean ± SEM. One-way ANOVA; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. MS, median survival. A, Created with BioRender. Raes, G. (2025) https://BioRender.com/v1isdrv .

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Construct

Journal: Cancer Research

Article Title: Depleting IL1R2 + Tumor-Infiltrating Regulatory T Cells with an ADCC-Prone Nanobody Construct Boosts the Efficacy of Anti–PD-1 Immunotherapy

doi: 10.1158/0008-5472.CAN-24-3095

Figure Lengend Snippet: IL1R2 + tiTreg depletion using the ADCC-prone construct 3 or the SDALIE-mutated, ADCC-enhanced construct 3 (E.construct3) synergizes with anti–PD-1 in E0771 tumor-bearing mice. A and B, Individual ( A ) and average ( B ) growth curves of E0771 tumors grown in WT mice ( n = 10) treated with the indicated combinations. C, Kaplan–Meier survival curves of WT mice ( n = 10) bearing E0771 tumors and treated with the indicated combinations, sacrificed at humane endpoint. D, The mice with regressed tumors were rechallenged at day 57 with E0771 in the contralateral mammary fat pad. E, Percentage of IL1R2 + tiTregs within total tiTregs and percentage of tiTregs within CD45 + cells of E0771 tumors in WT mice treated with the indicated combinations. F and G, Percentages of CD8 + T cells ( F ) and cDC1s and cDC2s ( G ) within CD45 + cells of E0771 tumors in WT mice treated with the indicated combinations. H, Average growth curves of E0771 tumors grown in WT mice ( n = 10) treated with the indicated combinations. I, Kaplan–Meier survival curves of WT mice ( n = 10) bearing E0771 tumors and treated with the indicated combinations, sacrificed at humane endpoint. J, Individual growth curves of E0771 tumors grown in WT mice ( n = 10) treated with the indicated combinations. K, The mice with regressed tumors were rechallenged at day 79 with E0771 in the contralateral mammary fat pad. For B and H , data are shown as mean ± SEM. Statistics: two-way ANOVA. For E–G , data are shown as mean ± SEM. Statistics: one-way ANOVA. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. MS, median survival; NA, not applicable.

Article Snippet: Briefly, two llamas were injected six times with 1-week interval with ±70 μg of the recombinant human IL1R2 fused to a His 6 tag (Sino Biological, cat. #10111-H08H) and with ±70 μg recombinant mouse IL1R2 fused to a His 6 tag (Sino Biological, cat. #51090-M08H) mixed with Gerbu LQ3000 (Gerbu Biotechnik).

Techniques: Construct