Journal: Biomedicines
Article Title: Resolvin D1 Modulates the Inflammatory Processes of Human Periodontal Ligament Cells via NF-κB and MAPK Signaling Pathways
doi: 10.3390/biomedicines13123038
Figure Lengend Snippet: RvD1 exerts its impact by regulating the NF-κB and MAPK signaling pathways. ( A ) RvD1 regulates the nuclear translocation of NF-κB. Compared to stimulation with LPS alone, the addition of RvD1 reduced the nuclear accumulation of NF-κB p65 (green). Nuclei are shown in blue (DAPI). Scale bar is 200 μm. WB of the NF-kB and IKBKB were analyzed. ( B ) IF of p-ERK. RvD1 inhibited the LPS-induced expression of p-ERK (green). The cell nucleus was counterstained with DAPI (blue). Scale bar is 200 μm. The banding pictures of JNK, p-ERK, ERK, p-p38, p38 and β-actin. WB analysis of JNK, p-ERK and p-p38. ( C ) Detection of IL-6 and IL-1β via ELISA (** p < 0.01; * p < 0.05; ns, not significant).
Article Snippet: The primary antibodies included: β-actin (1:1000, Proteintech, Rosemont, IL, USA), ALX/FPR2 (1:1000, Abcam, Cambridge, UK), TLR4, MyD88, p65, p-p65, IKBKB, JNK (1:1000, Proteintech, Rosemont, IL, USA), ERK (1:1000, Immunoway, Plano, TX, USA), p-ERK (1:1000, Bioworld, Louis Park, MN, USA), p38 (1:1000, Proteintech, Rosemont, IL, USA), p-p38 (1:1000, Abcam, Cambridge, UK).
Techniques: Protein-Protein interactions, Translocation Assay, IF-P, Expressing, Enzyme-linked Immunosorbent Assay