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(A) IFNβ and (B) <t>IFNλ3</t> proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).
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(A) IFNβ and (B) <t>IFNλ3</t> proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).
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il-28b -/- (ifnλ3 -/- ) mice  (Jackson Laboratory)
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(A) IFNβ and (B) <t>IFNλ3</t> proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).
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recombinant mouse ifnλ3  (Thermo Fisher)
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(A) IFNβ and (B) <t>IFNλ3</t> proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).
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Average 90 stars, based on 1 article reviews
recombinant mouse ifnλ3 - by Bioz Stars, 2026-09
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ifnλ3  (Thermo Fisher)
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Thermo Fisher ifnλ3
(A) IFNβ and (B) <t>IFNλ3</t> proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).
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(A) IFNβ and (B) IFNλ3 proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).

Journal: bioRxiv

Article Title: Modeling viral and bacterial infections in human lung organotypic systems reveals strain specific host responses

doi: 10.1101/2025.03.31.644992

Figure Lengend Snippet: (A) IFNβ and (B) IFNλ3 proteins detected in top channel washes recovered at 4 days post-infection. (n=5 independent experiments with 5 different organoid lines. Each dot color represents an organoid line. Median +/- interquartile range are plotted and Kruskal-Wallis tests are used for addressing statistically significant differences).

Article Snippet: For the IFNλ3 assay, the 21730-1 IgG1, kappa, mouse monoclonal antibody (PBL Assay Science) was used as a capture antibody after coating on paramagnetic beads (0.3 mg.mL -1 ), the MAB52591R IgG2a, mouse monoclonal antibody (R&D Systems, Minneapolis, MN, USA) was biotinylated (biotin/antibody ratio = 60/1) and used as the detector antibody at a concentration of 0.3 μg.mL -1 , and the 5259-IL-025/CF recombinant human IFNλ3 protein (R&D Systems) was used as calibrator.

Techniques: Infection