Journal: ACS Omega

Article Title: Analysis of Intracellular Fatty Acid Metabolism during Doxorubicin-Induced Senescence of MCF7 Cells Using Raman Imaging

doi: 10.1021/acsomega.5c09213

Figure Lengend Snippet: Visualization of intracellular biomolecular composition of senescent cells using hyperspectral Raman imaging. (A) Principal component analysis of hyperspectral Raman images of MCF7 human breast adenocarcinoma cells during proliferation and 10 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (B) Principal component analysis of hyperspectral Raman images of MCF7 human breast adenocarcinoma cells during proliferation and 15 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (C) Principal component analysis of hyperspectral Raman images of MCF7 human breast adenocarcinoma cells during proliferation and 18 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (D) Principal component analysis of hyperspectral Raman images of MCF7 human breast adenocarcinoma cells during proliferation and 25 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (E) Scatter plot of the projected loadings of the contribution of the Raman shifts to the principal components PC1 and PC2 in the PCA of hyperspectral Raman images of MCF7 human breast adenocarcinoma cells during DNA damage-mediated senescence after treatment with Doxo (150 nM). Red highlighted points show projected loadings of Raman shifts corresponding to CH 2 stretching (2850 cm –1 ). Green points show projected loadings of Raman shifts corresponding to CC or NCO stretching (1655 cm –1 ). Blue points show projected loadings of Raman shifts corresponding to CH 3 stretching (2920 cm –1 ), and yellow points show projected loadings of Raman shifts corresponding to CH 2 scissoring or CH 2 /CH 3 bending (1445 cm –1 ). (F) Cell-averaged peak intensity of the CH 2 stretching peak (2850 cm –1 ) obtained from hyperspectral Raman imaging of MCF7 human breast adenocarcinoma cells during DNA damage-mediated senescence ( n ≥ 20 cells). (G) Cell-averaged peak intensity of the CH 3 stretching peak (2920 cm –1 ) obtained from hyperspectral Raman imaging of MCF7 human breast adenocarcinoma cells during DNA damage-mediated senescence ( n ≥ 20 cells). (The standard deviation between replicates was plotted as error bars. Statistical significance was tested by the two-tailed Student’s t test assuming heteroscedastic distributions. *** p < 0.001, **** p < 0.0001.)

Article Snippet: Hyperspectral Raman imaging was done using an alpha 300 Ri system (WITec GmbH, Oxford Instruments) using the following parameters: objective: 40× air; laser: 532 nm; laser power: 60 mW; scan speed: 2 s/pixel; pixel size: 1 μm/pixel; detector grating: 600 mm –1 .

Techniques: Imaging, Standard Deviation, Two Tailed Test

Journal: ACS Omega

Article Title: Analysis of Intracellular Fatty Acid Metabolism during Doxorubicin-Induced Senescence of MCF7 Cells Using Raman Imaging

doi: 10.1021/acsomega.5c09213

Figure Lengend Snippet: Visualization of intracellular biomolecular composition of lipid-rich regions in senescent cells using hyperspectral Raman imaging. (A) Representative heatmaps of lipid-rich regions in MCF7 human breast adenocarcinoma cells during DNA damage-mediated senescence after treatment with Doxo (150 nM). (B) Principal component analysis of Raman spectra isolated from the lipid-rich regions of MCF7 human breast adenocarcinoma cells during proliferation and 10 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (C) Principal component analysis of Raman spectra isolated from the lipid-rich regions of MCF7 human breast adenocarcinoma cells during proliferation and 15 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (D) Principal component analysis of Raman spectra isolated from the lipid-rich regions of MCF7 human breast adenocarcinoma cells during proliferation and 18 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (E) Principal component analysis of Raman spectra isolated from the lipid-rich regions of MCF7 human breast adenocarcinoma cells during proliferation and 25 days after induction of DNA damage-mediated senescence ( n ≥ 20 cells). (F) Scatter plot of the projected loadings of the contribution of Raman shifts to the principal components PC1 and PC2 in the PCA of pixel-by-pixel Raman spectra obtained from the lipid-rich regions of MCF7 human breast adenocarcinoma cells during DNA damage-mediated senescence after treatment with Doxo (150 nM). Red highlighted points show projected loadings of Raman shifts corresponding to CH 2 stretching (2850 cm –1 ). Green points show projected loadings of Raman shifts corresponding to CC or NCO stretching (1655 cm –1 ). Blue points show projected loadings of Raman shifts corresponding to CH 3 stretching (2920 cm –1 ), and yellow points show projected loadings of Raman shifts corresponding to CH 2 scissoring or CH 2 /CH 3 bending (1445 cm –1 ). (G) Averaged peak intensity of the CH 2 stretching peak (2850 cm –1 ) in Raman spectra obtained from the lipid-rich regions of MCF7 human breast adenocarcinoma cells during DNA damage-mediated senescence ( n ≥ 20 cells). (The standard deviation between replicates was plotted as error bars. Statistical significance was tested by the two-tailed Student’s t test assuming heteroscedastic distributions. *** p < 0.001, **** p < 0.0001.)

Article Snippet: Hyperspectral Raman imaging was done using an alpha 300 Ri system (WITec GmbH, Oxford Instruments) using the following parameters: objective: 40× air; laser: 532 nm; laser power: 60 mW; scan speed: 2 s/pixel; pixel size: 1 μm/pixel; detector grating: 600 mm –1 .

Techniques: Imaging, Isolation, Standard Deviation, Two Tailed Test

Journal: ACS Omega

Article Title: Analysis of Intracellular Fatty Acid Metabolism during Doxorubicin-Induced Senescence of MCF7 Cells Using Raman Imaging

doi: 10.1021/acsomega.5c09213

Figure Lengend Snippet: Visualization of arachidonic acid metabolism in senescent MCF7 cells using hyperspectral Raman imaging of deuterated arachidonic acid. (A) Raman spectrum of 5,6,8,9,11,​12,14,15- d 8 arachidonic acid (AA- d 8 ) showing two modes of signature spectral shifts of (CC)D stretching in the biologically silent region at 2220 (minor peak) and 2254 cm –1 (major peak). (B) Intensity of the two (CC)D stretching peaks (2220 and 2254 cm –1 ) normalized to the intensity of the total lipid peak (CH 2 stretching, 2850 cm –1 ) measured by Raman spectroscopy at different time points during PTGS2-mediated metabolism of arachidonic acid in vitro ( n ≥ 15). (C) Visualization of the intracellular distribution of AA- d 8 in senescent MCF7 cells by hyperspectral Raman imaging. (D) Ratiometric heatmaps for visualization of the intracellular distribution of AA- d 8 in senescent MCF7 cells by hyperspectral Raman imaging. (E) Intensity of the two (CC)D stretching peaks (2220 and 2254 cm –1 ) normalized to the intensity of the total lipid peak (CH 2 stretching, 2850 cm –1 ) measured by hyperspectral Raman imaging in senescent MCF7 cells at different time points after the removal of PTGS2 (COX2) inhibitor (Cay-10404). (The standard deviation between replicates was plotted as error bars. Statistical significance was tested by the two-tailed Student’s t test assuming heteroscedastic distributions. *** p < 0.001, **** p < 0.0001.)

Article Snippet: Hyperspectral Raman imaging was done using an alpha 300 Ri system (WITec GmbH, Oxford Instruments) using the following parameters: objective: 40× air; laser: 532 nm; laser power: 60 mW; scan speed: 2 s/pixel; pixel size: 1 μm/pixel; detector grating: 600 mm –1 .

Techniques: Imaging, Raman Spectroscopy, In Vitro, Standard Deviation, Two Tailed Test

Journal: iScience

Article Title: Intelligent classification of dominant tree species in urban forests based on UAV hyperspectral remote sensing images

doi: 10.1016/j.isci.2026.115633

Figure Lengend Snippet: Hyperspectral image tree species identification results for various feature combinations

Article Snippet: Hyperspectral Image , This paper ; Mendeley Data , https://doi.org/10.17632/zsfshdgkvz.1 om/preview/zsfshdgkvz?a=1cdcecba-4bbb-4f38-9b80-faa245475f67.

Techniques: