Journal: Bioactive Materials

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

doi: 10.1016/j.bioactmat.2026.01.026

Figure Lengend Snippet: Gel-AgNA/MgGA MN remodel the microenvironment after TMI by regulating infection, inflammation, oxidative stress, and vascular disruption. (a) FISH (marker of bacteria, red) staining of harvested samples after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b, c) Quantitative analysis of bacterial coverage and fluorescence intensity of FISH (n = 9). (d) Heatmaps of DEGs related to inflammation and oxidative stress. (e) RT-PCR analysis showing the relative expression levels of IL-1, TNF-α, HIF-1α, and IL-6 (n = 3). (f) IF staining of iNOS (marker of M1 macrophages, red) and CD206 (marker of M2 macrophages, green). (g) Quantitative analysis of iNOS and CD206 fluorescence intensity. (h) RT-PCR analysis showing the relative expression levels of Arg1 and CD206. (i) IF staining of CD31 (marker of blood vessels, red). (j, k) RT-PCR analysis showing the relative expression levels of VEGF and HIF-1α (n = 3).

Article Snippet: According to the method reported in previous study [ ], Fluorescence In Situ Hybridization (FISH) test was conducted for bacteria (Servicebio, Eub338) to assess bacterial distribution.

Techniques: Infection, Disruption, Marker, Bacteria, Staining, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: STAR Protocols

Article Title: Protocol to measure transcriptional bursting of endogenous genes using high-throughput RNA-FISH

doi: 10.1016/j.xpro.2026.104441

Figure Lengend Snippet: Nascent RNA Florescence in-situ hybridization (nscFISH) marks transcription sites (A) Representative example of high-quality nscFISH detection showing stained nuclei with one (left), two (middle) or three (right) active transcription sites. (B and C) Illustrative examples of sub-optimal nscFISH. (B) High background noise Left: Comparative example of two cells in the same field where one has an active transcription site and the other has background signal. Right: Cell with an active transcription site but with a high background signal. (C) Cell with a bright fluorescent signal (yellow arrowhead) that is partly on the outside of the nucleus. Active transcription sites indicated by white arrowheads. Scale bar 10 μm.

Article Snippet: Humidified incubator for hybridization , Fisher Scientific , 15-015-2632.

Techniques: In Situ Hybridization, Staining