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huvecs viability  (Dojindo Labs)
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Dojindo Labs huvecs viability
FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated <t>HUVECs</t> treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. <t>(D)</t> <t>CCK-8</t> assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.
Huvecs Viability, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell viability assay huvec  (MedChemExpress)
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FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated <t>HUVECs</t> treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. <t>(D)</t> <t>CCK-8</t> assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.
Cell Viability Assay Huvec, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell viability human umbilical vein endothelial cells huvecs  (Procell Inc)
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FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated <t>HUVECs</t> treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. <t>(D)</t> <t>CCK-8</t> assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.
Cell Viability Human Umbilical Vein Endothelial Cells Huvecs, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell viability detection human vena cava endothelial cells huvecs  (Pasteur Institute)
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Pasteur Institute cell viability detection human vena cava endothelial cells huvecs
FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated <t>HUVECs</t> treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. <t>(D)</t> <t>CCK-8</t> assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.
Cell Viability Detection Human Vena Cava Endothelial Cells Huvecs, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huvec viability  (Beyotime)
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Beyotime huvec viability
FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated <t>HUVECs</t> treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. <t>(D)</t> <t>CCK-8</t> assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.
Huvec Viability, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huvecs viability  (Thermo Fisher)
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Thermo Fisher huvecs viability
FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated <t>HUVECs</t> treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. <t>(D)</t> <t>CCK-8</t> assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.
Huvecs Viability, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huvec viability  (MedChemExpress)
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MedChemExpress huvec viability
Effect of paeoniflorin (PF) on HUVECs. (A) PF chemical structure. (B) Evaluation of <t>HUVEC</t> <t>viability</t> using CCK-8 assay after exposure to differing concentrations of PF for 24 h. (C) Evaluation of HUVEC viability using CCK-8 assay after stimulation to different concentrations of TBHP for 24 h. (D) Representative images demonstrating cell morphology alterations in PF and TBHP co-treatment in HUVECs (scan bar, 100 μm). (E) Evaluation of HUVEC viability using CCK-8 assay after PF and TBHP co-treatment in HUVECs. Data: mean ± SD, * p < 0.05 and ** p < 0.01. n = 3.
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FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (D) CCK-8 assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.

Journal: Frontiers in immunology

Article Title: Lenvatinib improves anti-PD-1 therapeutic efficacy by promoting vascular normalization via the NRP-1-PDGFRβ complex in hepatocellular carcinoma.

doi: 10.3389/fimmu.2023.1212577

Figure Lengend Snippet: FIGURE 3 The concentration-escalation treatment with lenvatinib showed different phenotypes of vascular permeability and interaction with pericytes. (A) The permeability of the endothelial monolayer was assessed by the passage of Evans blue (EB)/albumin through the upper endothelial monolayer in the lower chamber (n=3 separate experiments). (B) Western blot analysis of the phosphorylation of VE-cadherin and VEGFR2 and total expression of VE-cadherin and VEGFR2 in the indicated HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (C) Representative IF staining images of VE-cadherin (green) in HUVECs treated with lenvatinib at different concentrations for 2 h, followed by stimulation with 60 ng/ml VEGF for 1 hour. (D) CCK-8 assay showing the proliferation of the indicated HBVPs with different treatments at different time points. (E) The OD450 value of the indicated HBVPs after different treatments for 72 h. (F) Representative images of the indicated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (G) Statistical graphs of migrated HBVPs assessed by the Transwell migration assay after different treatments for 72 h. (H, I) Fluorescently-labeled pericytes (green) were plated together with red-labeled endothelial cells (ECs) onto Matrigel at a 4:1 (EC: pericyte) ratio, cultured for 24 h (H) and 48 h (I), and the resulting vessel-like structures were analyzed by fluorescent microscopy. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001; ns indicates non-significant.

Article Snippet: HUVECs viability was determined using the cell counting kit-8 (CCK-8) cell viability assay (Dojindo Laboratories, Kumamoto, Japan).

Techniques: Concentration Assay, Permeability, Western Blot, Phospho-proteomics, Expressing, Staining, CCK-8 Assay, Transwell Migration Assay, Labeling, Cell Culture, Microscopy

Effect of paeoniflorin (PF) on HUVECs. (A) PF chemical structure. (B) Evaluation of HUVEC viability using CCK-8 assay after exposure to differing concentrations of PF for 24 h. (C) Evaluation of HUVEC viability using CCK-8 assay after stimulation to different concentrations of TBHP for 24 h. (D) Representative images demonstrating cell morphology alterations in PF and TBHP co-treatment in HUVECs (scan bar, 100 μm). (E) Evaluation of HUVEC viability using CCK-8 assay after PF and TBHP co-treatment in HUVECs. Data: mean ± SD, * p < 0.05 and ** p < 0.01. n = 3.

Journal: Frontiers in Pharmacology

Article Title: Paeoniflorin Suppresses TBHP-Induced Oxidative Stress and Apoptosis in Human Umbilical Vein Endothelial Cells via the Nrf2/HO-1 Signaling Pathway and Improves Skin Flap Survival

doi: 10.3389/fphar.2021.735530

Figure Lengend Snippet: Effect of paeoniflorin (PF) on HUVECs. (A) PF chemical structure. (B) Evaluation of HUVEC viability using CCK-8 assay after exposure to differing concentrations of PF for 24 h. (C) Evaluation of HUVEC viability using CCK-8 assay after stimulation to different concentrations of TBHP for 24 h. (D) Representative images demonstrating cell morphology alterations in PF and TBHP co-treatment in HUVECs (scan bar, 100 μm). (E) Evaluation of HUVEC viability using CCK-8 assay after PF and TBHP co-treatment in HUVECs. Data: mean ± SD, * p < 0.05 and ** p < 0.01. n = 3.

Article Snippet: HUVEC viability were evaluated with the Cell Counting Kit-8 (CCK-8) assay (MedChemExpress LLC; Monmouth Junction, NJ, United States), following operational guidelines.

Techniques: CCK-8 Assay