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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions <t>in</t> <t>THP-1</t> after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.
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USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions in THP-1 after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.

Journal: iScience

Article Title: Ultra short waves alleviate ulcerative colitis via TRPV2 mediated-macrophage polarization

doi: 10.1016/j.isci.2026.115929

Figure Lengend Snippet: USW treatment mainly regulates intracellular calcium levels in macrophages (A) Fluorescence imaging of calcium ions at 0, 10, 15, and 30 min in primary BMDM cells after USW treatment, visualized with Furo-4 AM with or without IV2-1. (B) Dynamics of calcium ion changes in primary BMDMs after USW treatment, visualized with Furo-4 AM. The panel quantitatively presents the fluorescence intensity over time, analyzed using software with or without IV2-1. (C) Fluorescence imaging of total calcium ions in THP-1 after USW treatment using GCaMP6s with or without nifedipine or U73122. (D) Fluorescence imaging of ER calcium ions in THP-1 after USW treatment using GCaMPer with or without nifedipine or U73122. (E) The pro-inflammatory cytokines mRNA levels ( Tnfα, Nos2, Il1β, Il6 ) in LPS-induced primary BMDMs were treated with USW treatment. E, One-way ANOVA, n = 7; ∗∗, p < 0.01. Data are represented as mean ± SEM.

Article Snippet: Human THP-1 cells , ATCC , TIB-202.

Techniques: Fluorescence, Imaging, Software