Journal: Genetics and Molecular Biology

Article Title: Human molecular cytogenetics: From cells to nucleotides

doi:

Figure Lengend Snippet: FISH with different types of probes and partial metaphases. (a) Whole chromosome 21 painting; (b) partial chromosome painting probe for the long arm of chromosome 9; (c) locus-specific probe for chromosome 4p16.3 (red) and Alfa satellite probe 4p11-q11(green); (d) subtelomeric probe for the short arm (red) and long arm (green) of chromosome 1; (e) human telomeric probes; and (f) Interphase-FISH with locus-specific SRY (sex-determining region Y) probe located in Yp11.31(red) and control probes for the X centromere (DXZ1) (blue) and for the heterochromatic block at Yq12 (green).

Article Snippet: The initial arrayed DNA segments could be larger (∼150 kb) human DNA segments inserted into a bacterial artificial chromosome (BAC clones) or bacterial/P1-derived artificial chromosomes (PAC clones) ( Snijders et al. , 2001 ; Fiegler et al. , 2003 ; Chung et al. , 2004 ; Ishkanian et al. , 2004 ).

Techniques: Control, Blocking Assay

Journal: Genetics and Molecular Biology

Article Title: Human molecular cytogenetics: From cells to nucleotides

doi:

Figure Lengend Snippet: Comparative Genomic Hybridization. (A) Conventional CGH analysis: a mixture of test DNA from a patient and a normal reference DNA labeled with different fluorochromes are hybridized to normal chromosome spreads ( top panel ). The left panel illustrates the hybridization pattern of chromosome 13. The interstitial segment of q-arm appears red, which indicates a loss of the region indicating rev ish dim (13q21q31). The right panel shows a graph of the ratio profiles of chromosome 13. The black line represents the balanced fluorescence intensities, and the red line is the threshold for loss, and the green line is the threshold for a gain of material. (B) Chromosome 4 array-CGH profile of a test DNA and a reference DNA. The figure shows a copy number loss corresponding to the segment of 4p16.3-p15.33 in a genomic segment with the median log2 ratio shifted to −1.0. The lower panel shows the 4p16.3-p15.33 region with the deletion segment and the genes present in this region.

Article Snippet: The initial arrayed DNA segments could be larger (∼150 kb) human DNA segments inserted into a bacterial artificial chromosome (BAC clones) or bacterial/P1-derived artificial chromosomes (PAC clones) ( Snijders et al. , 2001 ; Fiegler et al. , 2003 ; Chung et al. , 2004 ; Ishkanian et al. , 2004 ).

Techniques: Hybridization, Labeling, Fluorescence