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SHP2 Can Mediate the Expression of ROS/JNK/P53 Axis Related Proteins in MGC-803 Cells and <t>lncRNA</t> SNHG18 Can Specifically Target Inhibit miR-211-5p Expression. A: Western blot strips for p -SHP2, p -JNK, P53, and NOX4 and statistical graph of relative protein expression; B: Q-PCR detection of the expression of SNHG18 and miR-211-5p; C: Statistical results of the Dual-Luciferase reporter assay.**P < 0.01; ns P>0.05.
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Gene expression datasets used in this study.
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Gene expression datasets used in this study.
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DEL, DEM, and DEG screening. (A) Red represents upregulated lncRNAs/miRNAs/mRNAs <t>in</t> <t>HCM,</t> and blue represents downregulated lncRNAs/miRNAs/mRNAs in HCM, while grey represents those not significant. (B) 93 DELs (77 up-regulated and 16 down-regulated), 163 DEMs (91 up-regulated and 72 down-regulated), and 432 DEGs were overlapped. DEL, differentially expressed <t>long</t> <t>non-coding</t> <t>RNA;</t> DEM, differentially expressed microRNA; mRNA, messenger RNA; DEG, differentially expressed gene; HCM, hypertrophic cardiomyopathy.
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SHP2 Can Mediate the Expression of ROS/JNK/P53 Axis Related Proteins in MGC-803 Cells and lncRNA SNHG18 Can Specifically Target Inhibit miR-211-5p Expression. A: Western blot strips for p -SHP2, p -JNK, P53, and NOX4 and statistical graph of relative protein expression; B: Q-PCR detection of the expression of SNHG18 and miR-211-5p; C: Statistical results of the Dual-Luciferase reporter assay.**P < 0.01; ns P>0.05.

Journal: Heliyon

Article Title: SHP2 mediates the ROS/JNK/NFAT4 signaling pathway in gastric cancer cells prompting lncRNA SNHG18 to drive gastric cancer growth and metastasis via CAR-T cells

doi: 10.1016/j.heliyon.2024.e34008

Figure Lengend Snippet: SHP2 Can Mediate the Expression of ROS/JNK/P53 Axis Related Proteins in MGC-803 Cells and lncRNA SNHG18 Can Specifically Target Inhibit miR-211-5p Expression. A: Western blot strips for p -SHP2, p -JNK, P53, and NOX4 and statistical graph of relative protein expression; B: Q-PCR detection of the expression of SNHG18 and miR-211-5p; C: Statistical results of the Dual-Luciferase reporter assay.**P < 0.01; ns P>0.05.

Article Snippet: Another dataset for gastric cancer-related mRNA, GSE109476, was downloaded from the Arraystar Human LncRNA microarray V2.0 platform with probe GPL24530.

Techniques: Expressing, Western Blot, Luciferase, Reporter Assay

lncRNA SNHG18 Can Promote CAR-T Cell Exhaustion and Apoptosis. A: Protein strips of BRD4, PD-L1, IDO1, Caspase-3, Caspase-8, and Bax; B: Statistical graph of relative protein expression levels of BRD4, PD-L1, IDO1, Caspase-3, Caspase-8, and Bax.**P < 0.01; ns P>0.05.

Journal: Heliyon

Article Title: SHP2 mediates the ROS/JNK/NFAT4 signaling pathway in gastric cancer cells prompting lncRNA SNHG18 to drive gastric cancer growth and metastasis via CAR-T cells

doi: 10.1016/j.heliyon.2024.e34008

Figure Lengend Snippet: lncRNA SNHG18 Can Promote CAR-T Cell Exhaustion and Apoptosis. A: Protein strips of BRD4, PD-L1, IDO1, Caspase-3, Caspase-8, and Bax; B: Statistical graph of relative protein expression levels of BRD4, PD-L1, IDO1, Caspase-3, Caspase-8, and Bax.**P < 0.01; ns P>0.05.

Article Snippet: Another dataset for gastric cancer-related mRNA, GSE109476, was downloaded from the Arraystar Human LncRNA microarray V2.0 platform with probe GPL24530.

Techniques: Expressing

lncRNA SNHG18 Can Inhibit the Proliferation and Migration of MGC-803 Cells. A: Diagram and cell gap statistical graph of the wound-healing assay; B: Diagram of transwell assay and statistical graph of the number of migrating cells; C: CCK8 assay result graph.**P < 0.01; ns P>0.05.

Journal: Heliyon

Article Title: SHP2 mediates the ROS/JNK/NFAT4 signaling pathway in gastric cancer cells prompting lncRNA SNHG18 to drive gastric cancer growth and metastasis via CAR-T cells

doi: 10.1016/j.heliyon.2024.e34008

Figure Lengend Snippet: lncRNA SNHG18 Can Inhibit the Proliferation and Migration of MGC-803 Cells. A: Diagram and cell gap statistical graph of the wound-healing assay; B: Diagram of transwell assay and statistical graph of the number of migrating cells; C: CCK8 assay result graph.**P < 0.01; ns P>0.05.

Article Snippet: Another dataset for gastric cancer-related mRNA, GSE109476, was downloaded from the Arraystar Human LncRNA microarray V2.0 platform with probe GPL24530.

Techniques: Migration, Wound Healing Assay, Transwell Assay, CCK-8 Assay

SHP2 in gastric cancer cells mediates the ROS/JNK/NFAT4 signaling pathway, inducing lncRNA SNHG18 to drive growth and metastasis through the miR-211-5p/BRD4 axis in CAR-T cells.

Journal: Heliyon

Article Title: SHP2 mediates the ROS/JNK/NFAT4 signaling pathway in gastric cancer cells prompting lncRNA SNHG18 to drive gastric cancer growth and metastasis via CAR-T cells

doi: 10.1016/j.heliyon.2024.e34008

Figure Lengend Snippet: SHP2 in gastric cancer cells mediates the ROS/JNK/NFAT4 signaling pathway, inducing lncRNA SNHG18 to drive growth and metastasis through the miR-211-5p/BRD4 axis in CAR-T cells.

Article Snippet: Another dataset for gastric cancer-related mRNA, GSE109476, was downloaded from the Arraystar Human LncRNA microarray V2.0 platform with probe GPL24530.

Techniques:

Gene expression datasets used in this study.

Journal: Medicine

Article Title: Characterization of lncRNA-associated ceRNA network to uncover novel potential biomarkers in coronary artery disease

doi: 10.1097/MD.0000000000035913

Figure Lengend Snippet: Gene expression datasets used in this study.

Article Snippet: GSE69587 , Yue Cai , GPL15314 Arraystar Human LncRNA microarray V2.0 (Agilent_033010 Probe Name version) , 3:3 , 2015.

Techniques: Expressing, Microarray

DEL, DEM, and DEG screening. (A) Red represents upregulated lncRNAs/miRNAs/mRNAs in HCM, and blue represents downregulated lncRNAs/miRNAs/mRNAs in HCM, while grey represents those not significant. (B) 93 DELs (77 up-regulated and 16 down-regulated), 163 DEMs (91 up-regulated and 72 down-regulated), and 432 DEGs were overlapped. DEL, differentially expressed long non-coding RNA; DEM, differentially expressed microRNA; mRNA, messenger RNA; DEG, differentially expressed gene; HCM, hypertrophic cardiomyopathy.

Journal: Journal of Thoracic Disease

Article Title: SNHG 12 and hsa-miR-140-5P may play an important role in the ceRNA network related to hypertrophic cardiomyopathy

doi: 10.21037/jtd-23-189

Figure Lengend Snippet: DEL, DEM, and DEG screening. (A) Red represents upregulated lncRNAs/miRNAs/mRNAs in HCM, and blue represents downregulated lncRNAs/miRNAs/mRNAs in HCM, while grey represents those not significant. (B) 93 DELs (77 up-regulated and 16 down-regulated), 163 DEMs (91 up-regulated and 72 down-regulated), and 432 DEGs were overlapped. DEL, differentially expressed long non-coding RNA; DEM, differentially expressed microRNA; mRNA, messenger RNA; DEG, differentially expressed gene; HCM, hypertrophic cardiomyopathy.

Article Snippet: LncRNA expression profiling related to HCM was conducted for 7 HCM patients and 5 disease-free individuals who were included in GSE68316 (platform: GPL20113 Capital Bio Human LncRNA Microarray v2.0; CapitalBio, Beijing, China) and for 28 HCM patients and 9 healthy donors who were included in GSE130036 (platform: GPL20795, HiSeq X Ten; Illumina, San Diego, CA, USA).

Techniques: