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SOX2 is highly expressed in hepatocellular carcinoma (HCC) cell lines. (a-c) qRT-PCR and Western blot analysis of SOX2 mRNA and protein expression levels in normal liver epithelial (THLE2) and HCC (Huh7) cell lines. (d-f) qRT-PCR and Western blot validation of transfection efficiency in Huh7 cells transfected with shRNA targeting SOX2 (shSOX2) or SOX2 overexpression plasmids. n = 6; ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, qRT-PCR: Quantitative reverse transcription polymerase chain reaction. mRNA: messenger RNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh-NC: shRNA-negative control; Oe-NC: overexpression plasmid negative control.

Journal: CytoJournal

Article Title: SOX2 promotes the progression of hepatocellular carcinoma by targeting FOXJ3 and activating the PI3K/AKT pathway to regulate autophagy and mitophagy

doi: 10.25259/Cytojournal_164_2025

Figure Lengend Snippet: SOX2 is highly expressed in hepatocellular carcinoma (HCC) cell lines. (a-c) qRT-PCR and Western blot analysis of SOX2 mRNA and protein expression levels in normal liver epithelial (THLE2) and HCC (Huh7) cell lines. (d-f) qRT-PCR and Western blot validation of transfection efficiency in Huh7 cells transfected with shRNA targeting SOX2 (shSOX2) or SOX2 overexpression plasmids. n = 6; ✶ ✶ ✶ P <0.001. SOX2: SRY-box transcription factor 2, qRT-PCR: Quantitative reverse transcription polymerase chain reaction. mRNA: messenger RNA; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; sh-NC: shRNA-negative control; Oe-NC: overexpression plasmid negative control.

Article Snippet: Human normal liver epithelial cells (THLE-2, CL-0833) and HCC cells (Huh7, CL-0120) were purchased from Procell (Wuhan, Hubei, China).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Biomarker Discovery, Transfection, shRNA, Over Expression, Reverse Transcription, Polymerase Chain Reaction, Negative Control, Plasmid Preparation