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Generation and characterization of <t>iPSC-derived</t> cardiomyocytes. A. Schematic overview of the experimental design. B. Reprogramming factor expression in control and MRPL44 patient-derived iPSCs after reprogramming (OCT4; green, NANOG; red and TRA-1-60; green) (Scale bar; 200µm) C. Cardiac marker positivity of iPSC <t>derived</t> <t>CMs.</t> Immunofluorescence, cardiac troponin (cTnT; green) and nuclei (DAPI, blue) (Scale bar; 50 µm). D. Purification of iPSC derived cardiomyocytes. Representative FACS analysis shows 91.9% cTnT positive cells after purification.
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Generation and characterization of iPSC-derived cardiomyocytes. A. Schematic overview of the experimental design. B. Reprogramming factor expression in control and MRPL44 patient-derived iPSCs after reprogramming (OCT4; green, NANOG; red and TRA-1-60; green) (Scale bar; 200µm) C. Cardiac marker positivity of iPSC derived CMs. Immunofluorescence, cardiac troponin (cTnT; green) and nuclei (DAPI, blue) (Scale bar; 50 µm). D. Purification of iPSC derived cardiomyocytes. Representative FACS analysis shows 91.9% cTnT positive cells after purification.

Journal: bioRxiv

Article Title: Nutrient-dependent pathology in mitochondrial hypertrophic cardiomyopathy model

doi: 10.1101/2025.09.24.678132

Figure Lengend Snippet: Generation and characterization of iPSC-derived cardiomyocytes. A. Schematic overview of the experimental design. B. Reprogramming factor expression in control and MRPL44 patient-derived iPSCs after reprogramming (OCT4; green, NANOG; red and TRA-1-60; green) (Scale bar; 200µm) C. Cardiac marker positivity of iPSC derived CMs. Immunofluorescence, cardiac troponin (cTnT; green) and nuclei (DAPI, blue) (Scale bar; 50 µm). D. Purification of iPSC derived cardiomyocytes. Representative FACS analysis shows 91.9% cTnT positive cells after purification.

Article Snippet: Contracting cells were observed from day 8 onwards. iPSC derived CMs were purified using magnetic beads as per manufacturer’s instructions (#130-110-188, Miltenyi Biotec) on day 15-20 of differentiation.

Techniques: Derivative Assay, Expressing, Control, Marker, Immunofluorescence, Purification

Impaired lipid homeostasis in patient CMs A. Lipid droplet analysis. Immunostaining of neutral lipid droplets in MRPL44 and Control iPSC-CMs by Lipidtox. (cTnT, yellow; Lipidtox, red; TOM20, green; DAPI, blue; scale: 50 µm) B. Quantification of droplets per cell between patient and control CMs. (n=1273 CMs) *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Student’s t-test) C. Gene expression levels of lipid transporter CD36, LDLR, ACSL1 and PDK4 gene in different media conditions. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Wald test)

Journal: bioRxiv

Article Title: Nutrient-dependent pathology in mitochondrial hypertrophic cardiomyopathy model

doi: 10.1101/2025.09.24.678132

Figure Lengend Snippet: Impaired lipid homeostasis in patient CMs A. Lipid droplet analysis. Immunostaining of neutral lipid droplets in MRPL44 and Control iPSC-CMs by Lipidtox. (cTnT, yellow; Lipidtox, red; TOM20, green; DAPI, blue; scale: 50 µm) B. Quantification of droplets per cell between patient and control CMs. (n=1273 CMs) *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Student’s t-test) C. Gene expression levels of lipid transporter CD36, LDLR, ACSL1 and PDK4 gene in different media conditions. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Wald test)

Article Snippet: Contracting cells were observed from day 8 onwards. iPSC derived CMs were purified using magnetic beads as per manufacturer’s instructions (#130-110-188, Miltenyi Biotec) on day 15-20 of differentiation.

Techniques: Immunostaining, Control, Gene Expression