Journal: Cell Communication and Signaling : CCS

Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage

doi: 10.1186/s12964-025-02175-2

Figure Lengend Snippet: FG-4592 lowers iFGF23 level in CKD rats and CKD patients. Parameters measured include ( A ) Serum intact fibroblast growth factor 23 (iFGF23) levels in CKD rats. B Serum total FGF23 levels in CKD rats. C Serum iFGF23 levels in CKD patients. D Serum total FGF23 levels in CKD patients. E Scatter plots with linear regression analysis demonstrating a significant correlation between blood urea nitrogen (BUN) and iFGF23 levels in CKD rats. F Scatter plots with linear regression analysis showing a significant correlation between Scr and iFGF23 levels in CKD rats. G Scatter plots with linear regression analysis revealing a significant correlation between iFGF23 levels and the percentage of fibrotic area in the kidney of CKD rats. H Scatter plots with linear regression analysis indicating a significant correlation between iFGF23 levels and Collagen 1 staining intensity in the kidney of CKD rats. I Scatter plots with linear regression analysis illustrating a significant correlation between iFGF23 levels and Fibronectin staining intensity in the kidney of CKD rats. J Scatter plots with linear regression analysis demonstrating a significant correlation between iFGF23 levels and α-SMA staining intensity in the kidney of CKD rats. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for human iFGF23 or total FGF23 (Quidel, cat#: 60–6600 and cat#: 60–6100). iFGF23 ELISAs detect full-length, biologically active FGF23 that can exert phosphaturia, whereas total FGF23 ELISAs detect both iFGF23 and its C-terminal fragments and therefore provide a measure of the total amount of circulating plasma FGF23.

Techniques: Staining

Journal: Cell Communication and Signaling : CCS

Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage

doi: 10.1186/s12964-025-02175-2

Figure Lengend Snippet: iFGF23 mediates FG-4592-induced inhibition of tubular cell fibrogenesis. HK-2 cells were pre-treated with TGF-β1 (10 ng/mL) for 24 h, followed by treatment with recombinant FGF23 (rFGF23, 50 ng/mL) or vehicle for an additional 24 h. Parameters measured include: A COL1A1 mRNA expression levels in HK-2 cells, as determined by quantitative PCR (qPCR). B TGFB1 mRNA expression levels in HK-2 cells, as determined by qPCR. C Collagen 1 protein expression levels in HK-2 cells, as assessed by Western blot analysis. D Schematic illustration of the co-culture system using UMR-106 cells and HK-2 cells in a Transwell setup. E mRNA expression levels of Fn1 , Acta2 , and Col1a1 in HK-2 cells, as quantified by qPCR. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for human iFGF23 or total FGF23 (Quidel, cat#: 60–6600 and cat#: 60–6100). iFGF23 ELISAs detect full-length, biologically active FGF23 that can exert phosphaturia, whereas total FGF23 ELISAs detect both iFGF23 and its C-terminal fragments and therefore provide a measure of the total amount of circulating plasma FGF23.

Techniques: Inhibition, Recombinant, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Co-Culture Assay

Journal: Cell Communication and Signaling : CCS

Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage

doi: 10.1186/s12964-025-02175-2

Figure Lengend Snippet: FG-4592 ameliorates TIF by inhibiting iFGF23-WNT5A pathway. Parameters measured include ( A ) Hierarchical clustering analysis revealing distinct mRNA expression profiles among the experimental groups. Yellow and blue shades indicate expression levels above and below the relative mean, respectively, across all samples. B Gene Ontology (GO) enrichment analysis. C Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. D and F mRNA and protein expression levels of Wnt5a and β-catenin in the following groups: sham, CKD + vehicle, and CKD + FG-4592. E and G mRNA and protein expression levels of Wnt5a and β-catenin in the following groups: CKD + FG-4592 + vehicle and CKD + FG-4592 + rFGF23. H and I Representative immunohistochemical images showing Wnt5a and β-catenin expression in kidney tissues across different experimental groups. J mRNA expression levels of COL1A1 , ACTA1 , WNT5A , and CTNNB1 in HK-2 cells following WNT5A siRNA interference, as determined by quantitative PCR (qPCR). K Protein expression levels of Collagen 1, Wnt5a, and β-catenin in HK-2 cells after WNT5A siRNA interference, as assessed by Western blot analysis. Scale bars: 50 μm. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for human iFGF23 or total FGF23 (Quidel, cat#: 60–6600 and cat#: 60–6100). iFGF23 ELISAs detect full-length, biologically active FGF23 that can exert phosphaturia, whereas total FGF23 ELISAs detect both iFGF23 and its C-terminal fragments and therefore provide a measure of the total amount of circulating plasma FGF23.

Techniques: Expressing, Immunohistochemical staining, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage

doi: 10.1186/s12964-025-02175-2

Figure Lengend Snippet: FG-4592 lowers iFGF23 levels through Furin-mediated cleavage. Parameters measured include ( A ) mRNA expression levels of Furin , Galnt3 , and Fam20c in bone tissues from the following groups: sham, CKD + vehicle, and CKD + FG-4592, as determined by quantitative PCR (qPCR). B Protein levels of HIF-1α, Furin, and intact fibroblast growth factor 23 (iFGF23) in bone tissues treated with FG-4592 or vehicle, as assessed by Western blot analysis. C Representative images of hematoxylin and eosin (HE) staining of bone tissues (scale bars: 20 μm), along with IHC staining for HIF-1α and iFGF23 (scale bars: 50 μm) and Furin (scale bars: 100 μm). D UMR-106 cells were pretreated with 10 nM 1,25(OH) 2 D 3 , and the expression of FGF23 mRNA was analyzed using real-time PCR. E Western blot analysis was performed to measure iFGF23 protein expression in UMR-106 cells. Cells were pretreated with indoxyl sulfate (IS, 0.5 mM) or vehicle, followed by treatment with FG-4592 (50 μM) or vehicle for 24 h. F Levels of iFGF23 and total FGF23 in the cell supernatant were quantified using an ELISA assay kit. G Western blot analysis was used to evaluate the protein expression of iFGF23 and Furin in UMR-106 cells. H Motif map of the Furin promoter region, highlighting the HIF-1 binding site. I ChIP assays demonstrated HIF-1 binding to the Furin promoter, which was enhanced by FG-4592 stimulation (50 μM for 24 h). J Protein expression levels of Furin and iFGF23 in UMR-106 cells following Furin siRNA interference, as determined by Western blot analysis. K Levels of iFGF23 and total FGF23 in the cell supernatant were measured using an ELISA assay kit after Furin siRNA interference. Data are presented as means ± SD ( n = 5). Statistical significance is denoted as follows: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for human iFGF23 or total FGF23 (Quidel, cat#: 60–6600 and cat#: 60–6100). iFGF23 ELISAs detect full-length, biologically active FGF23 that can exert phosphaturia, whereas total FGF23 ELISAs detect both iFGF23 and its C-terminal fragments and therefore provide a measure of the total amount of circulating plasma FGF23.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Staining, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Cell Communication and Signaling : CCS

Article Title: The Roxadustat (FG-4592) ameliorates tubulointerstitial fibrosis by promoting intact FGF23 cleavage

doi: 10.1186/s12964-025-02175-2

Figure Lengend Snippet: Schematic illustration of FG-4592 ameliorating TIF. During CKD, FG-4592 transcriptionally upregulates Furin expression, which subsequently cleaves iFGF23, leading to a reduction in iFGF23 levels and amelioration of TIF

Article Snippet: The iFGF23 concentration was examined on plasma collected from rats under fed conditions using ELISA assays specific for rat iFGF23 (Quidel, cat#: 60–6800). iFGF23 and cFGF23 concentration were examined on plasma collected from rats under fed conditions using ELISA assays specific for rat total FGF23 (Quidel, cat#: 60–6300). iFGF23 and cFGF23 concentration were examined on plasma collected from CKD patients under fed conditions using ELISA assays specific for human iFGF23 or total FGF23 (Quidel, cat#: 60–6600 and cat#: 60–6100). iFGF23 ELISAs detect full-length, biologically active FGF23 that can exert phosphaturia, whereas total FGF23 ELISAs detect both iFGF23 and its C-terminal fragments and therefore provide a measure of the total amount of circulating plasma FGF23.

Techniques: Expressing

Journal: Transplantation Direct

Article Title: Klotho and Fibroblast Growth Factor 23 Are Independent of Vitamin D, and Unlike Vitamin D, Are Not Associated With Graft- and Patient Survival After Kidney Transplantation

doi: 10.1097/TXD.0000000000001522

Figure Lengend Snippet: Baseline characteristics 8 wk posttransplant, for all patients as well as divided on the basis of baseline plasma vitamin D levels (8 wk posttransplant).

Article Snippet: Both iFGF23 and sKlotho were measured in ethylenediaminetetraacetic acid (EDTA) plasma. iFGF23 was measured with the commercially available human iFGF23 enzyme-linked-immunosorbent serologic assay (ELISA) kits from Immutopics International (San Clemente, CA), and sKlotho was measured with the commercially available human sKlotho ELISA kit from Immuno-Biological Laboratories (IBL, GmbH, Japan).

Techniques: Clinical Proteomics

Journal: Transplantation Direct

Article Title: Klotho and Fibroblast Growth Factor 23 Are Independent of Vitamin D, and Unlike Vitamin D, Are Not Associated With Graft- and Patient Survival After Kidney Transplantation

doi: 10.1097/TXD.0000000000001522

Figure Lengend Snippet: 8 wk and 1 y data.

Article Snippet: Both iFGF23 and sKlotho were measured in ethylenediaminetetraacetic acid (EDTA) plasma. iFGF23 was measured with the commercially available human iFGF23 enzyme-linked-immunosorbent serologic assay (ELISA) kits from Immutopics International (San Clemente, CA), and sKlotho was measured with the commercially available human sKlotho ELISA kit from Immuno-Biological Laboratories (IBL, GmbH, Japan).

Techniques:

Journal: Endocrine Connections

Article Title: Cholecalciferol supplementation effectively improved tertiary hyperparathyroidism, FGF23 resistance and lowered coronary calcification score: a prospective study

doi: 10.1530/EC-22-0123

Figure Lengend Snippet: Change of laboratory parameters in KT group at four time points ( n = 209).

Article Snippet: Serum concentrations of intact FGF23 (iFGF23) (Boster Biological Technology., Ltd., Wuhan, China) and KLA (Immuno-Biological Laboratories Co., Ltd., Gunma, Japan) were measured using ELISA methods with the available commercial kits.

Techniques:

Journal: Endocrine Connections

Article Title: Cholecalciferol supplementation effectively improved tertiary hyperparathyroidism, FGF23 resistance and lowered coronary calcification score: a prospective study

doi: 10.1530/EC-22-0123

Figure Lengend Snippet: Comparison of laboratory parameters between vitamin D-treated group and -untreated group at month 6 ( n = 209).

Article Snippet: Serum concentrations of intact FGF23 (iFGF23) (Boster Biological Technology., Ltd., Wuhan, China) and KLA (Immuno-Biological Laboratories Co., Ltd., Gunma, Japan) were measured using ELISA methods with the available commercial kits.

Techniques: Comparison

Journal: Endocrine Connections

Article Title: Cholecalciferol supplementation effectively improved tertiary hyperparathyroidism, FGF23 resistance and lowered coronary calcification score: a prospective study

doi: 10.1530/EC-22-0123

Figure Lengend Snippet: Comparison of laboratory parameters and CACS between vitamin D-treated group and -untreated group at month 12 ( n = 209).

Article Snippet: Serum concentrations of intact FGF23 (iFGF23) (Boster Biological Technology., Ltd., Wuhan, China) and KLA (Immuno-Biological Laboratories Co., Ltd., Gunma, Japan) were measured using ELISA methods with the available commercial kits.

Techniques: Comparison

Journal: Endocrine Connections

Article Title: Cholecalciferol supplementation effectively improved tertiary hyperparathyroidism, FGF23 resistance and lowered coronary calcification score: a prospective study

doi: 10.1530/EC-22-0123

Figure Lengend Snippet: Change of (A) iFGF23 (logarithmic scale; Ln iFGF23), (B) KLA, (C) BCTX and (D) P1NP in treated and untreated group within 12 months posttransplant. * P < 0.05 compared between groups. ** P < 0.01 compared between groups. # P < 0.05 compared with HC group. † P < 0.05 compared with CKD group.

Article Snippet: Serum concentrations of intact FGF23 (iFGF23) (Boster Biological Technology., Ltd., Wuhan, China) and KLA (Immuno-Biological Laboratories Co., Ltd., Gunma, Japan) were measured using ELISA methods with the available commercial kits.

Techniques:

Journal: Endocrine Connections

Article Title: Cholecalciferol supplementation effectively improved tertiary hyperparathyroidism, FGF23 resistance and lowered coronary calcification score: a prospective study

doi: 10.1530/EC-22-0123

Figure Lengend Snippet: Multivariate regression analysis of risk factors for THPT and CACS at month 12.

Article Snippet: Serum concentrations of intact FGF23 (iFGF23) (Boster Biological Technology., Ltd., Wuhan, China) and KLA (Immuno-Biological Laboratories Co., Ltd., Gunma, Japan) were measured using ELISA methods with the available commercial kits.

Techniques: