Journal: Frontiers in Immunology

Article Title: Comprehensive characterization of SLC41A3 identifies it as an immune-related prognostic biomarker and therapeutic target in hepatocellular carcinoma

doi: 10.3389/fimmu.2026.1861310

Figure Lengend Snippet: SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

Article Snippet: The human HCC cell lines Hep3B and Huh7 were purchased from Procell Biotechnology.

Techniques: Western Blot, Knockdown, Transfection, shRNA, CCK-8 Assay, Migration, Wound Healing Assay

Journal: Journal of Xenobiotics

Article Title: Antioxidant Therapy Reverses Hepatotoxicity Induced by Microcystin-LR in a Cellular Model of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)

doi: 10.3390/jox16030076

Figure Lengend Snippet: Treatment of Hep3B hepatocytes with 500 µM of oleic acid induces MASLD-like conditions. Representative pictures of ORO stained Hep3B cells ( A , B ) under 20x magnification shows accumulation of lipid droplets in cells treated with 500 μM of oleic acid, as well as significant increases in the levels of triglycerides ( C ) and free fatty acids ( D ) as compared to Vehicle ( n = 3; * p ≤ 0.05, *** p ≤ 0.001).

Article Snippet: Human hepatocellular carcinoma Hep3B (liver epithelial) cells were purchased from American Type Culture Collection (ATCC) (Cat. No. HB-8064, ATCC, Manassas, VA, USA).

Techniques: Staining

Journal: Journal of Xenobiotics

Article Title: Antioxidant Therapy Reverses Hepatotoxicity Induced by Microcystin-LR in a Cellular Model of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)

doi: 10.3390/jox16030076

Figure Lengend Snippet: Targeted antioxidant treatment using pNaKtide and NAC significantly reduces MC-LR-induced hepatotoxicity in Hep3B hepatocytes. Quantitative PCR analysis of markers of hepatotoxicity—SerpinE1 ( A ) and OSMR ( B ); inflammation—TNFα ( C ) and Tgf-β1 ( D ); fatty acid metabolism—CD36 ( E ) and hepatic injury—ALPL ( F ) were all significantly upregulated on treatment with MC-LR (10 µM) in the presence of oleic acid (500 µM) as compared to those in the absence of oleic acid, except for CD36, which was upregulated in the presence of MC-LR alone. These markers were significantly downregulated in cells treated with targeted antioxidants. Quantification of SERPINE1/PAI-1 protein by ELISA ( G ) in the Hep3B supernatants mirrored the genetic expression of SerpinE1 and was also reduced with antioxidant treatment; similarly, 8-OHdG ( H ), a marker of oxidative stress, followed the same trend with MC-LR causing a significant oxidative stress in the presence of oleic acid as compared to MC-LR alone, and this was reduced with antioxidant treatment. ( n = 3, with duplicates; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).

Article Snippet: Human hepatocellular carcinoma Hep3B (liver epithelial) cells were purchased from American Type Culture Collection (ATCC) (Cat. No. HB-8064, ATCC, Manassas, VA, USA).

Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing, Marker

Journal: Journal of Xenobiotics

Article Title: Antioxidant Therapy Reverses Hepatotoxicity Induced by Microcystin-LR in a Cellular Model of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)

doi: 10.3390/jox16030076

Figure Lengend Snippet: Targeted antioxidant treatment significantly improves MC-LR metabolism in hepatocytes. Mass spectrometric-based quantification of MC-LR (green bars) and MC-LR Cysteine (yellow bars) in Hep3B cell pellet ( A ) and Hep3B cell supernatant ( B ) showed a decrease in the levels of MC-LR in cells that were treated with antioxidants as compared to the MASLD cells exposed to the toxin. On the other hand, the levels of detoxified MC-LR Cysteine were improved on treatment with antioxidants (inset to right shows MC-LR-Cysteine data on a separate scale). ( n = 3; * p ≤ 0.05, **** p ≤ 0.0001).

Article Snippet: Human hepatocellular carcinoma Hep3B (liver epithelial) cells were purchased from American Type Culture Collection (ATCC) (Cat. No. HB-8064, ATCC, Manassas, VA, USA).

Techniques:

Journal: bioRxiv

Article Title: Light-dependent cell fixing with DNA-targeting fluorophores

doi: 10.64898/2026.03.27.714905

Figure Lengend Snippet: (A) Localization of PAL (green) at mtDNA within MitoRed ® -stained mitochondria (red) in live Hep3B cells prior to irradiation (scale bar: 10 µm, crop: 5 µm). (B) Top: snapshots of time-lapse PAL nuclear fluorescence upon WF irradiation (scale bar: 20 µm). Bottom: PAL labeling upon irradiation at distinct phases of the cell cycle ( a : interphase; b : prophase; c : prometaphase; d : metaphase; e : anaphase. Scale bar: 5 µm). Right : kinetics of PAL fluorogenesis during 5 min irradiation. Mean intensities are rescaled from 0 to 1 within a 95% confidence interval. (C) PAL nuclear fluorescence (in green) of a targeted cell islet within a population of live PAL-treated cells upon WF irradiation (PAL + hv) (R1: dotted line; R2: dashed line; NA: non-affected area. Scale bar: 50 µm). Crops in R1 (orange squares) showing stable nuclear morphology and persistence up to 9 days post-irradiation within a live-cell proliferative environment. Crops in R2 (blue squares) showing compromised nuclei. Calculated irradiation powers were 3.5 to 15 W/cm 2 in R1 from limit to center of the beam. Only evanescent or residual scattered light was present in R2 (scale bar: 20 µm). (D) Co-staining of PAL-treated cells (green) with propidium iodide (PI, red) or NucView ® 530 Red Caspase-3 dye (NucView, magenta). Top: PI stains R1 cells (dotted lines), showing a permeabilized status immediately post-irradiation (d0). After 24 h, R2 cells show important delayed PI staining demonstrating a compromised state. Bottom: NucView faintly stains R1 cells 24 h post-irradiation, showing strong signal in compromised R2 cells. The apoptosis inducer staurosporine is used as a positive control. (E) Quantification of nuclear size in different conditions: live PAL-treated cells without irradiation (PAL), FA-fixed cells (FA), PAL-free irradiated cells (hv) or PAL-treated irradiated cells (PAL + hv, R1 and R2) (n>200 per condition). Data shown in violin plots reflecting size distribution, circles indicate the medians and whiskers indicate standard deviations.

Article Snippet: The human cell line Hep3B was obtained from ATCC ® (HB-8064), the human cell lines T24, PC3 and C4-2B were a courtesy of Pr.

Techniques: Staining, Irradiation, Fluorescence, Labeling, Positive Control

Journal: bioRxiv

Article Title: Light-dependent cell fixing with DNA-targeting fluorophores

doi: 10.64898/2026.03.27.714905

Figure Lengend Snippet: (A) Left: Optofixing procedure of a whole Hep3B cell population using a portable LED lamp towards biochemical analysis. Top right: Visualization of abundance of precipitates (UVA irradiation). Right bottom: Corresponding SDS-PAGE analysis of soluble protein contents following treatments with formaldehyde (FA), 4-hydroxynonenal (4HNE), acrolein (ACR) or LED irradiation in presence or absence of PAL. (B) Left panel: WF imaging of PAL nuclear fluorogenesis at day 0 after irradiation by a WF microscope. Right panel: stability of fixed state after 3 days of PAL-treated cells in presence of inhibitors (scale bar: 10µm). (C) Left: fluorogenesis kinetics during 5 min irradiation with conditions described in B . Fluorescence intensities were normalized as [(I-Io)/Io]. Right : quantification of nuclear sizes with conditions described in B (n>60 per condition) 3 days post-irradiation. ( D) Lipid peroxidation (LPO)-quantification in PAL-treated cells using BODIPY™ 581/591-C11 probe. Top left: representative image of probe staining 30 min after irradiation of PAL-treated cells in zones NA, R1 and R2. Top right: plot of ratiometric measurements along the yellow dashed line. Red curve represents Red/Ox ratio, green curve represents the Ox/Red ratio. Bottom: quantification of the oxidation ratio (Ox/Red) 30 min after irradiation of PAL-treated cells (R1, R2) compared to irradiated cells in absence of PAL (hv) or cells treated with PAL without irradiation (PAL) (n>100 per condition). (E) ROS quantification in PAL-treated cells using ROS Brite TM 670 probe. Top: representative image of ROS staining (in magenta) obtained after irradiation of PAL-treated cells (in green) in R1 and R2 (scale bar: 10 µm). Bottom: Quantification of ROS Brite fluorescence intensity in the same conditions than D .

Article Snippet: The human cell line Hep3B was obtained from ATCC ® (HB-8064), the human cell lines T24, PC3 and C4-2B were a courtesy of Pr.

Techniques: Irradiation, SDS Page, Imaging, Microscopy, Fluorescence, Staining

Journal: bioRxiv

Article Title: Light-dependent cell fixing with DNA-targeting fluorophores

doi: 10.64898/2026.03.27.714905

Figure Lengend Snippet:

Article Snippet: The human cell line Hep3B was obtained from ATCC ® (HB-8064), the human cell lines T24, PC3 and C4-2B were a courtesy of Pr.

Techniques: Labeling