Journal: Nature Communications

Article Title: Membrane lipid nanodomains modulate HCN pacemaker channels in nociceptor DRG neurons

doi: 10.1038/s41467-024-54053-z

Figure Lengend Snippet: a , b Representative phasor plots showing FRET between L10-CFP and hHCN2-YFP expressed in small DRG neurons before and after 10 nM paclitaxel treatment. c Summary data of the effects of the β-CD and paclitaxel on the FRET between L10-CFP and hHCN2-YFP. Data shown are mean ± s.e.m., n = 7 for control and β-CD conditions, n = 8 for paclitaxel conditions, ** p = 1e-6 for β-CD and ** p = 4e-6 for paclitaxel compared with the control. One-way ANOVA (no adjustment) was used. d Cartoon showing the design of the FRET sites in the VSD of human HCN2 channels and the representative phasor plot showing FRET between donor AF-488 labeled to the HCN2-L323TAG site and the FRET acceptor AF-555 labeled to the HCN2-T240TAA site. e Representative phasor plot of the same FRET pair as in panel ( d ) following acute 5 mM β-cyclodextrin treatment. f Summary data of the effect of the β-CD on the FRET efficiency of hHCN2-L323TAG/T240TAA channels. Data shown are mean ± s.e.m., n = 5 cells for the control and n = 4 cells after β-CD, ** p = 0.002 using two-sided student’s t -test. g Representative phasor plot of the same FRET pair as in panels ( d ) and ( e ), following acute 5 mM β-cyclodextrin treatment with 120 mM KCl in the bath. h Summary data of the effect of the β-CD on the FRET efficiency as shown in panel ( g ). Data shown are mean ± s.e.m., n = 7 cells for the control and n = 6 cells with β-CD, p = 0.054 using two-sided student’s t -test.

Article Snippet: Wild-type human HCN2 construct was synthesized by VectorBuilder (Chicago, IL).

Techniques: Control, Labeling

Journal: iScience

Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells

doi: 10.1016/j.isci.2022.105402

Figure Lengend Snippet: qPCR data evaluating the expression of key Purkinje markers in control and PURK-cocktail-treated cells The PURK-cocktail treatment induced a different genetic profile, which resembled closely to that of native cardiac Purkinje cells on both AC16-CM and iPSC-CM compared to the control. Data corresponds to mean ± SEM of n=3. ∗,∗∗,∗∗∗,∗∗∗∗ corresponds to p < 0.05, 0.01, 0.001, and 0.0001 in a two-Way ANOVA test. “ns” corresponds to “not significant.”

Article Snippet: HCN2 Human qPCR Primer Pair , OriGene , cat# HP205124.

Techniques: Expressing, Control

Journal: iScience

Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells

doi: 10.1016/j.isci.2022.105402

Figure Lengend Snippet:

Article Snippet: HCN2 Human qPCR Primer Pair , OriGene , cat# HP205124.

Techniques: Recombinant, Transfection, Knock-Out, Multiplex sample analysis, SYBR Green Assay, Reverse Transcription, Derivative Assay, Plasmid Preparation, Software

Journal: iScience

Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells

doi: 10.1016/j.isci.2022.105402

Figure Lengend Snippet: qPCR program

Article Snippet: HCN2 Human qPCR Primer Pair , OriGene , cat# HP205124.

Techniques: Activation Assay

Journal: iScience

Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells

doi: 10.1016/j.isci.2022.105402

Figure Lengend Snippet:

Article Snippet: HCN2 Human qPCR Primer Pair , OriGene , cat# HP205124.

Techniques: Recombinant, Transfection, Knock-Out, SYBR Green Assay, Derivative Assay, Plasmid Preparation, Software